Primer3 (Whitehead Institute/MIT Center for Genome Research)
Very popular primer design tool for designing primers for PCR, hybridization.
Added: Sat Jul 20 2002 Reviews: 0
Primerfinder (Univ. of Texas-SW Med. Ctr)
Easy to use, free, online primer design program
Added: Sat Jul 20 2002 Reviews: 0
MethPrimer (Long-Cheng Li, UCSF)
A free online program for designing primers for methylation specific PCR (MSP) and bisulfite sequencing PCR.
Added: Thu May 29 2003 Reviews: 0
Primo (Chang Bioscience)
Primo online is a friendly PCR primer design tool. It reduces PCR noise by lowering the probability of random primering. Batch mode allows designing for multiple sequences. Users can also calculate the melting temperature using the nearest neighbor model.