小動物體內可見光三維成像技術研究進展
楊華瑜1，韓 彧2，董洪瑩2，趙春林2*
(1 中國醫學科學院 中國協和醫科大學 北京協和醫院 肝臟外科， 北京 100730；
2 北京龍脈得- 冷泉港生物技術有限公司， 北京 100084)
摘 要：活體動物體內可見光成像是采用生物發光和熒光為標記物，利用靈敏的儀器來監控活體動物體內的細胞活動、蛋白表達情況和基因行為。近年來，可見光成像在生物醫學的各個方面得到了廣泛的應用。隨著成像技術和檢測儀器的不斷發展，現已從平面二維成像逐漸發展為立體三維成像。
三維成像技術在靶點的空間定位、與器官的關系，及絕對定量方面都有了很大的進展。 本文就三維成像技術的原理、應用和發展前景進行了簡要的綜述。
關鍵詞：體內光學成像；三維活體成像；生物發光；熒光；腫瘤轉移
中圖分類號：R445.1; Q631.11 文獻標識碼：A
Current developments of 3-D optical in vivo imaging technology in animal
YANG Hua-yu1, HAN Yu2, DONG Hong-ying2, ZHAO Chun-lin2*
(1Department of Liver Surgery, PUMCH, CAMS, Beijing 100730, China;
2Longmed, Inc/CS-Biotech, Inc., Beijing 100084, China)
Abstract: Bioluminescence and fluorescence have been proved to be a
versatile and useful tool for animal in vivoimaging. Optical imaging
employs sensitive instrument to monitor the activities of cell, protein
and gene in vivo.It has been used widely in biomedical research and drug
discoveries. With the recent development of the technology and
instruments, the 2Dimentional imaging has developed to 3Dimential
imaging in vivo. 3-D imaging will provide researchers not only detailed
spatial information of the targets, its relationship with animal organs,
but more quantitative results in vivo. This paper reviews of the
current principles, developments and applications of the 3-D optical in
vivo imaging technologies.