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  • 結論

    基于對本研究中氟喹諾酮化合物的特征離子化的觀察,使用UPLC IMS MSE進行方法開發是合理的。

      ■ 使用離子淌度,可將同一分子,不同質子化物質實現神奇的分離。

      ■ 顯示并鑒別了同分子,不同位點質子化合物的碎片譜圖。

      ■ 得到每個組分的母離子MS及其MSE碎片譜圖

      ■ HDMSE觀察結果可以解釋在同一實驗室內監測特定MRM離子隊獲得的研究對象報告結果有時 會出現差異的原因。

      ■ 漂移時間值可作為保留時間、母離子精確質量數和碎片精確質量數譜圖之外的又一個鑒定條件。

      ■ 離子淌度分離可以有效地將目標峰從基質干擾中分離出來,而不需要對復雜樣品進行純化及色譜分離。


    參考文獻
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    2.  FDA Center for Veterinary Medicine, June 2, 1997, FDA Order Prohibits  Extralabel Use Of Fluoroquinolones And Glycopeptides.
    3.  Official Journal of the European Union, L268:29-43, Regulation (EC) No  1831/2003 of the European Parliament and of the Council of 22 September  2003 on additives for use in animal nutrition.
    4.  Official Journal of the European Union, L24:1-8 Annex I, Council Regulation  (EEC) No 2377/90 of 26 June 1990 laying down a Community procedure  for the establishment of maximum residue limits of veterinary medicinal  products in foodstuffs of animal origin.
    5.  Verdon E, Couedor P, Roudaut B, Sandérs PJ. Multiresidue Method for  Simultaneous Determination of Ten Quinolone Antibacterial Residues in  Multimatrix/Multispecies Animal Tissues by Liquid Chromatography with  Fluorescence Detection: Single Laboratory Validation Study. AOAC Inter.  2005; 88:1179-1192.
    6.  Kaufmann A, Butcher P, Maden K, Widmer M, Giles K, Uria D. Are liquid  chromatography/electrospray tandem quadrupole fragmentation ratios  unequivocal confirmation criteria? Rapid Commun. Mass Spectrom. 2009;   23: 985-998.
    7.  Mol HG, Zomer P, de Koning M. Qualitative aspects and validation of a  screening method for pesticides in vegetables and fruits based on liquid  chromatography coupled to full scan high resolution (Orbitrap) mass  spectrometry. Anal Bioanal Chem. 2012; 403:2891-2908.
    8.  Croley TR, White KD, Callahan JH, Musser SM. T he chromatographic role   in high resolution mass spectrometry for non-targeted analysis.   J Am Soc Mass Spectrom. 2012; 23:1569.
    9.  Commission Decision (2002/657EEC) Official Journal of the European  Communities 2002. 10.  Lalli PM, Iglesias BA, Toma HE, de Sá GF, Daroda RJ, Silva Filho JC,  Szulejko JE, Araki K, Eberlin MN. Protomers: formation, separation and  characterization via travelling wave ion mobility mass spectrometry. J Mass  Spectrom. 2012; 47(6):712-9. 11.  Eberlin MN, Lali PM, Nachtigall FM, Riccio MF, de Sa GF, Daroda RJ, de  Souza V, Campuzano I, Souza GHMF. SYNAPT HDMS: Improving Ion Mobility  Separation by Increasing Drift-Gas Polarizability. Waters Technical Note  720003201en. 2009 October. 12.  Jurneczko E, Kalapothakis J, Campuzano IDG, Morris M, Barran PE. Effects   of drift gas on collision cross sections of a protein standard in linear drift   tube and traveling wave ion mobility mass spectrometry. Anal Chem.   2012; 84(20):8524-31. 13.Asbury GR, Hill HH. Using different drift gases to change separation factors  (alpha) in ion mobility spectrometry. Anal. Chem. 2000; 72(3): 580-4. 14.  Fasciotti M, Sanvido GB, Santos VG, Lalli PM, McCullagh M, de Sá GF,   Daroda RJ, Petera MG, Eberlina MN. J Mass Spectrom. 2012; 47(12):1643-7.


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  • 调性视频