結論
基于對本研究中氟喹諾酮化合物的特征離子化的觀察,使用UPLC IMS MSE進行方法開發是合理的。
■ 使用離子淌度,可將同一分子,不同質子化物質實現神奇的分離。
■ 顯示并鑒別了同分子,不同位點質子化合物的碎片譜圖。
■ 得到每個組分的母離子MS及其MSE碎片譜圖
■ HDMSE觀察結果可以解釋在同一實驗室內監測特定MRM離子隊獲得的研究對象報告結果有時 會出現差異的原因。
■ 漂移時間值可作為保留時間、母離子精確質量數和碎片精確質量數譜圖之外的又一個鑒定條件。
■ 離子淌度分離可以有效地將目標峰從基質干擾中分離出來,而不需要對復雜樣品進行純化及色譜分離。
參考文獻
1. Final Decision of the Commissioner,
Docket # 2000N-I 57 1 October 24, 2000, Withdrawal of the approval of
the new animal drug application for enrofloxacin in poultry. Department
of Health and Human Services, U.S. Food and Drug Administration.
2.
FDA Center for Veterinary Medicine, June 2, 1997, FDA Order Prohibits
Extralabel Use Of Fluoroquinolones And Glycopeptides.
3. Official
Journal of the European Union, L268:29-43, Regulation (EC) No 1831/2003
of the European Parliament and of the Council of 22 September 2003 on
additives for use in animal nutrition.
4. Official Journal of the
European Union, L24:1-8 Annex I, Council Regulation (EEC) No 2377/90 of
26 June 1990 laying down a Community procedure for the establishment
of maximum residue limits of veterinary medicinal products in
foodstuffs of animal origin.
5. Verdon E, Couedor P, Roudaut B,
Sandérs PJ. Multiresidue Method for Simultaneous Determination of Ten
Quinolone Antibacterial Residues in Multimatrix/Multispecies Animal
Tissues by Liquid Chromatography with Fluorescence Detection: Single
Laboratory Validation Study. AOAC Inter. 2005; 88:1179-1192.
6.
Kaufmann A, Butcher P, Maden K, Widmer M, Giles K, Uria D. Are liquid
chromatography/electrospray tandem quadrupole fragmentation ratios
unequivocal confirmation criteria? Rapid Commun. Mass Spectrom. 2009;
23: 985-998.
7. Mol HG, Zomer P, de Koning M. Qualitative aspects
and validation of a screening method for pesticides in vegetables and
fruits based on liquid chromatography coupled to full scan high
resolution (Orbitrap) mass spectrometry. Anal Bioanal Chem. 2012;
403:2891-2908.
8. Croley TR, White KD, Callahan JH, Musser SM. T he
chromatographic role in high resolution mass spectrometry for
non-targeted analysis. J Am Soc Mass Spectrom. 2012; 23:1569.
9.
Commission Decision (2002/657EEC) Official Journal of the European
Communities 2002. 10. Lalli PM, Iglesias BA, Toma HE, de Sá GF, Daroda
RJ, Silva Filho JC, Szulejko JE, Araki K, Eberlin MN. Protomers:
formation, separation and characterization via travelling wave ion
mobility mass spectrometry. J Mass Spectrom. 2012; 47(6):712-9. 11.
Eberlin MN, Lali PM, Nachtigall FM, Riccio MF, de Sa GF, Daroda RJ, de
Souza V, Campuzano I, Souza GHMF. SYNAPT HDMS: Improving Ion Mobility
Separation by Increasing Drift-Gas Polarizability. Waters Technical
Note 720003201en. 2009 October. 12. Jurneczko E, Kalapothakis J,
Campuzano IDG, Morris M, Barran PE. Effects of drift gas on collision
cross sections of a protein standard in linear drift tube and
traveling wave ion mobility mass spectrometry. Anal Chem. 2012;
84(20):8524-31. 13.Asbury GR, Hill HH. Using different drift gases to
change separation factors (alpha) in ion mobility spectrometry. Anal.
Chem. 2000; 72(3): 580-4. 14. Fasciotti M, Sanvido GB, Santos VG, Lalli
PM, McCullagh M, de Sá GF, Daroda RJ, Petera MG, Eberlina MN. J Mass
Spectrom. 2012; 47(12):1643-7.