電泳時的DNA分子量Marker和Agarose濃度的選擇

DNA片段長度

Agarose濃度 

使用Marker種類

200 bp以下

3%以上

　фX174-Hae Ⅲ digest DNA Marker
　фX174-Hinc Ⅱ digest DNA Marker
　DL500?DNA Marker
　DL1,000?DNA Marker
　DL2,000?DNA Marker
　20 bp DNA Ladder Marker
　50 bp DNA Ladder Marker
　100 bp DNA Ladder Marker
　150 bp DNA Ladder Marker
　Wide Range DNA Marker (100~6,000)

200 bp~700 bp

2%~3%

　фX174-Hae Ⅲ digest DNA Marker
　фX174-Hinc Ⅱ digest DNA Marker
　DL500? DNA Marker
　DL1,000? DNA Marker
　DL2,000? DNA Marker
　DL5,000? DNA Marker
　50 bp DNA Ladder Marker
　100 bp DNA Ladder Marker
　150 bp DNA Ladder Marker
　200 bp DNA Ladder Marker
　250 bp DNA Ladder Marker
　500 bp DNA Ladder Marker
　Wide Range DNA Marker (100~6,000)

700 bp~1,500 bp

1%~2%

　λ-EcoT14Ⅰdigest DNA Marker
　фX174-Hae Ⅲ digest DNA Marker
　фX174-Hinc Ⅱ digest DNA Marker
　DL2,000? DNA Marker
　DL5,000? DNA Marker
　DL10,000? DNA Marker
　50 bp DNA Ladder Marker
　100 bp DNA Ladder Marker
　150 bp DNA Ladder Marker
　200 bp DNA Ladder Marker
　250 bp DNA Ladder Marker
　500 bp DNA Ladder Marker
　Wide Range DNA Marker (100~6,000)
　Wide Range DNA Marker (500~12,000)
　Wide Range DNA Marker (500~15,000)

1,500 bp~5,000 bp

0.7%~1%

　λ-EcoT14Ⅰdigest DNA Marker
　λ-Hind Ⅲ digest DNA Marker
　DL5,000? DNA Marker
　DL10,000? DNA Marker
　DL15,000? DNA Marker
　Supercoiled DNA Ladder Marker
　200 bp DNA Ladder Marker
　250 bp DNA Ladder Marker
　500 bp DNA Ladder Marker
　Wide Range DNA Marker (100~6,000)
　Wide Range DNA Marker (500~12,000)
　Wide Range DNA Marker (500~15,000)
　1 Kbp DNA Ladder Marker

 5,000 bp以上

0.7%以下

　λ-EcoT14Ⅰdigest DNA Marker
　λ-Hind Ⅲ digest DNA Marker
　DL10,000? DNA Marker
　DL15,000? DNA Marker
　Supercoiled DNA Ladder Marker
　Wide Range DNA Marker (500~12,000)
　Wide Range DNA Marker (500~15,000)
　1 Kbp DNA Ladder Marker