· Histological techniques (William H. Heidcamp)
Very detailed guide to histological techniques, like fixation, dehydration, embedment and subsequent sectioning.
· Cryosectioning (House Ear Institute)
A detailed description of how to produce high quality cryosections of fixed, cryoprotected biological material.
· Cryostat sectioning (Klymkowsky Lab)
· Cryosectioning (Mike A. Dyer)
· Wax embedding/sectioning (Klymkowsky Lab)
· Sectioning Stained Embryo (XMMR)
· Counting Sectioned Cells (Xiaoxi Zhuang)
Cell number is often an important index of functions. The main factor which complicates the determination of cell number is that tissue preparation involves sectioning, a process that often splits cellular structures into two or more parts. Direct counting, therefore, results in overcounts. A number of cell counting correction schemes is described here.