· Comparative Genomic Hybridization (CGH) CGH is a molecular Cytogenetic method of screening a tumor for genetic changes. The alterations are classified as DNA gains and losses and reveal a characteristic pattern that includes mutations at chromosomal and subchromosomal levels. This protocol gives detailed procedures on CGH as follows:
Metaphase chromosome preparation
DNA preparation by cryostom tissue dissection
DNA labeling by nick translation
Hybridization
DNA detection
Image capture
Image analysis
DAPI chromosome identification
· Comparative Genomic Hybridization (CGH)
Detailed protocol for CGH, including slide and probe preparation, detection and staining
Direct CGH (Waldman Lab)
Indirect CGH (Waldman Lab)
Nick Translation of DNA for CGH (Waldman Lab)