Gram Staining (+\-) (William H. Heidcamp)
Gram-Staining Procedure (MEDIC, U of Texas)
Very nice and detailed method description for Gram staining
Acid-Fast Stain (Putt's Method)
To detect the presence of acid-fast mycobacteria in tissue sections
Smear Preparation (MEDIC, U of Texas)
The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure. A smear can be prepared from a solid or broth medium.
Alkaline Phosphatase and Phospholipase C Assay (Hancock Lab) (Accessible only by Internet Explorer)
Bacteria Agglutination Assay (Hancock Lab) (Accessible only by Internet Explorer)
Bacterial agglutination is performed using either P. aeruginosa cells autoclaved at 120oC for 1 hour, then centrifuged and resuspended in saline or live P. aeruginosa cells grown to mid log phase, then centrifuged and resuspended in saline.
Carbohydrate Assay(Hancock Lab) (Accessible only by Internet Explorer)
This assay is used to determine the relative amounts of LPS CHO present in a given strain and can be done on one set of samples and then scanned at the usr/localious wavelengths for reasonable data on the 3 types of sugars.
Dintrophenylation Assay for Polymixin B (Hancock Lab) (Accessible only by Internet Explorer)
Endotoxin Assay(Hancock Lab) (Accessible only by Internet Explorer)
This assay measures the shift in the absorbance maximum of the dye due to binding to endotoxin
Indirect Immunfluoresence (Hancock Lab) (Accessible only by Internet Explorer)
Liposome Swelling Assay (Hancock Lab) (Accessible only by Internet Explorer)
Also including method for liposome Isolation and liposome preparation
Preparation of E Coli Protein for SDS-PAGE (Hahn Lab)
Simplified method for analyzing E coli protein