應用案例三：多環芳烴
儀器方法：
Injector: Split/splitless, 1 min, 320℃ , 1.5 μL
injection volume with Merlin seal
Carrier gas: He, 0.6 mL/min, const. flow
Column: TRACE ? -50MS, 10 m x 0.1 mm x 0.1 μm
Oven Temp. Program: 140 ℃ , 1 min
10 ℃ /min to 240℃
5 ℃ /min to 270℃
30 ℃ /min to 280℃
4 ℃ /min to 290℃
30 ℃ /min to 315℃
3 ℃ /min to 330℃
Transfer line 300℃
ion source 280℃
Ionization: EI, 45 eV pos.
Scan Mode: Multiple ion detection mode (MID)
Resolution: 8,000, 10% valley definition
Cycle Time: 0.8 s/scan
參考文獻
[Application Note: 30126]
Fast GC/HRMS Quantification of 16 EC Priority PAH ComponentsKarl Speer,
Technical University Dresden, Institute of Food Chemistry, Dresden,
Germany, Katja Ziegenhals, Wolfgang Jira, Federal Research Centre for
Nutrition and Food (BfEL), Kulmbach, Germany,
Hans-Joachim Huebschmann, Thermo Fisher Scientific, Bremen, Germany
Contents
of polycyclic aromatic hydrocarbons (PAH) and phenolic substances in
Frankfurter-type sausages depending on smoking conditions using glow
smoke
Margarete P?hlmann , Alexander Hitzel , Fredi Schw?gele , Karl Speer , Wolfgang Jira
Polycyclic
aromatic hydrocarbons (PAH) and phenolic substances in meat products
smoked with different types of wood and smoking spices Alexander Hitzel ,
Margarete P?hlmann , Fredi Schw?gele , Karl Speer , Wolfgang Jira
應用案例四：短鏈氯化石蠟
SCCP C 10 H 17 Cl 5 –“CP3”標準物質色譜圖
Sample Preparation
樣品準備和標準物質由 Environment Canada, Burlington ON, Canada 提供。
1. 標準物質 SCCP C 10 H 17 Cl 5 – “CP3”
2. 混合物 SCCPs C10-13 55.5% Cl 環己烷
3. 包含 C13 內標的基質提取樣品
Gas Chromatography
GC Column：Trace Gold TR-5MS
30m x 0.25mm, 0.1 μm film
GC Liner：Single taper splitless
Injector temp： 220 ℃
Splitless time：3 minutes
Transfer line：220℃
Column Flow：1 mLmin -1 , constant flow
Mass Spectrometry
Electron capture negative chemical ionisation
was used to analyse SCCPs.
EC NCI Buffer Gas：Isobutane and ammonia
Source Pressure：6x10-4 mBar
Electron engery：90 eV
Emission current：0.3 mA
Ion source temp：80-95 ℃
Scan Multiple Ion Detection (MID)
參考文獻
Analysis of Short Chain
Chlorinated Paraffins (SCCPs) using High Resolution Mass Spectrometry
and Negative Chemical Ionization (NCI GC-HRMS)
Frank Theobald1, Heinz
Mehlmann2, Dirk Krumwiede2 & Kyle D’Silva2 1. Environmental
Consulting, Cologne, Germany 2. POPs Center of Excellence, Thermo Fisher
Scientific,Bremen, Germany
應用案例五：荷爾蒙 類固醇及農藥
● EPA Method 1698: Steroids and Hormones in Water, Soil, Sediment,and Biosolids by HRGC/HRMS
高分辨氣相色譜與高分辨磁質譜聯用分析水體、土壤、沉積物及生物體中的類固醇和荷爾蒙
● EPA Method 1699: Pesticides in Water,Soil, Sediment, Biosolids, and Tissue by HRGC/HRMS
高分辨氣相色譜與高分辨磁質譜聯用分析水體、土壤、沉積物及生物體中的農藥
參考文獻
● EPA Method 1698: Steroids and Hormones in Water, Soil, Sediment,and Biosolids by HRGC/HRMS
● EPA Method 1699: Pesticides in Water,Soil, Sediment, Biosolids, and Tissue by HRGC/HRMS