43、 Plasma Substitute Medium (代血漿培養基)
Sucrose (蔗糖) 12.5-15% Peptone (蛋白胨) 0.5%
KH2PO4 0.03% Na2HPO4.12H2O 0.14%
pH 7.0-7.2
44、Skim Milk Medium (脫脂牛奶培養基) ATCC 183
脫脂牛奶 100g 去離子水 1000ml
適用范圍:保加利亞乳桿菌、嗜熱乳酸鏈球菌
45、Pea Agar (豌豆瓊脂)
Glucose (葡萄糖) 1% Peptone (蛋白胨) 0.5%
NaCl 0.5% Agar (瓊脂) 2.0%
Pea infusion (counted by NH2) [豌豆浸汁(以NH2計)] 12-14 mg∕100
pH (before sterilization) [消前] 7.2-7.5
[Note]: Preparetion of pea infusion (豌豆浸汁的制法) :
Preparetion
900g of pea. Put them into a 5L bottle, and add 3L of tap water. Adjust
pH to 4.3-4.5 by using of sulphuric acid. Add 30ml of chloroform for
antisepsis. Plug with rubber stopper and bind up the bottle mouth with
gauge in order to avoid that the stopper fall off due to gas pressure.
Place the bottle at 37℃ for 7-10 days. Filter the infusion, then boil
the filtrate to escape the chloroform. Filter again Distribution and
autoclave at 120℃ for 30 minutes. Store at cool contition .
(稱取白豌豆900克,置于5公升廣口瓶中,加自來水3公升,用硫酸調pH至4.3-4.5,再加氯仿30毫升(防腐),加膠塞并用紗布扎口(防止沖脫),放37℃恒溫室7-10天。取出用濾紙過濾,濾液煮沸20分鐘,以除氯仿,并再次以濾紙過濾,除沉淀。濾液分裝,于120℃30分鐘滅菌。后冷藏備用。)
The standard quality of pea infusion as following (豌豆浸汁質量標準):
Aminonitrogen(氨基酸): 160-200 mg∕100ml
Soluble phosphoruse(溶磷): 400-600 μg∕ml
Color(色澤): auratus,transparent (金黃、透明)
46、Wheat Bran Medium (麥麩瓊脂)
Wheat bran (麥麩) 3.6% (NH4)2HPO4 0.3%
K2HPO4 0.02% MgSO4.7H2O 0.01%
Agar (瓊脂) 2% pH 7.0
適用范圍:金霉素鏈霉菌、紅霉素鏈霉菌
47、Glucose Yeast Extract Agar (葡萄糖、酵母膏瓊脂)
Glucose (葡萄糖) 10g Yeast extract (酵母膏) 10g
Water (水) 1000ml Agar (瓊脂) 15g
pH 7.2
48、MMN Medium (MMN培養基)
CaCl2 0.05g MgSO4 0.15g
NaCl 0.025g FeCl3 (1%) 1.2ml
KH2PO4 0.5g Vitamib B1 (硫胺素) 100μg
(NH4)2HPO4 0.25g Wort (12 Be′) 100ml
Glucose (葡萄糖) 10-15g Citric acid (檸檬酸) 0.2g
Distilled water (蒸餾水) 900ml pH 5.5 (左右)
適用范圍:蜜環菌
49、Starch Agar (淀粉瓊脂)
Soluble starch (可溶性淀粉) 10g NaNO3 1g
MgCO3 1g K2HPO4 0.3g
NaCl 0.5g Agar (瓊脂) 20g
Distilled water (蒸餾水) 1000ml
50、5 Be′Wort Agar (5 Be′麥芽汁瓊脂)
適用范圍:園弧青霉、少根根霉、日本根霉、爪哇根霉
51、5 Be′Koji Extract Agar (5 Be′米曲汁瓊脂)
適用范圍:華根霉、三寶壟根霉
52、發根農桿菌培養基(YEB)
Beef extract (牛肉浸膏) 5g/L Yeast extract (酵母膏) 1g/L
Peptone (蛋白胨) 5g/L Sucrose (蔗糖) 5g/L
MgSO4.7H2O 0.4g/100ml Agar (瓊脂) 1.5g/100ml
pH 7.4
適用范圍:青枯假單胞菌
53、青枯菌培養基 (Tm)
Peptone (蛋白胨) 10g Casein (干酪素水解物產) 1g
Glucose (葡萄糖) 5g Ddhro (純水) 1000ml
Agar(瓊脂) 15g pH 7.0
54、YM (Yeast Malt Agar)
酵母膏 3.0g 麥芽汁 3.0g
蛋白胨 5.0g 瓊脂 20.0g
蒸餾水 1000ml
55、ISP 4
可溶性淀粉 10.0g K2HPO4 1.0g
MgSO4.7H2O 1.0g NaCl 1.0g
(NH4)2SO4 2.0g CaCO3 2.0g
FeSO4.7H2O 0.001g MnCl2.7H2O 0.001g
瓊脂 20.0g 蒸餾水 1000ml
56、Trypton Soy Agar
胰蛋白胨 17.0g 大豆胨 3.0g
葡萄糖 2.5g 瓊脂 20.0g
NaCl 5.0g K2HPO4 2.5g
蒸餾水 1000ml
57、NA (Nutrient Agar)
牛肉膏 3.0g 蛋白胨 5.0g
瓊脂 20.0g 蒸餾水 1000ml
pH 6.6-7.0
58、甘露(糖)醇瓊脂 (ATCC 1)
酵母膏 5.0g 蛋白胨 3.0g
甘露(糖)醇 25.0g 瓊脂 15.0g
蒸餾水 1000ml
59、Marine瓊脂 2216 (ATCC 2)
蛋白胨 5.0g 酵母膏 1.0g
檸檬酸鐵 0.1g 溴化鉀 0.08g
NaCl 19.45g MgCl2 8.8g
Na2SO4 3.24g CaCl2 1.8g
KCl 0.55g NaHCO3 0.16g
NH4NO3 0.0016g Na2HPO4 0.008g
氯化鍶 0.034g 硼酸 0.022g
硅酸鈉 0.004g 氟化鈉 0.0024g
瓊脂 15.0g 蒸餾水 1000ml
pH 7.4-7.8
60、兔血瓊脂(ATCC 4)
牛心(取其浸液) 500.0g 胰蛋白胨 (Tryptose) 10.0g
NaCl 5.0g 瓊脂 15.0g
蒸餾水 1000ml pH 6.6-7.0
61、孢子形成培養基 (ATCC 5)
酵母膏 1.0g 牛肉膏 1.0g
胰蛋白胨 2.0g 葡萄糖 10.0g
FeSO4 微量 瓊脂 15.0g
蒸餾水 1000ml pH 7.2
62、Trypticase Soy Agar (ATCC 18)
Trypticase Soy Broth 30.0g 瓊脂 15.0g
蒸餾水 1000ml
63、YPAD (ATCC 109)
酵母膏 10.0g 蛋白胨 20.0g
葡萄糖 20.0g 瓊脂 20.0g
蒸餾水 1000ml pH 7.0
64、SP (ATCC 432)
棉子糖 1.0g 蔗糖 1.0g
半乳糖 1g 可溶性淀粉 5g
水解酪蛋白 2.5g 瓊脂 20.0g
MgSO4.7H2O 0.5g K2HPO4 0.25g
蒸餾水 1000ml pH 自然
65、胰酪胨大豆酵母浸膏瓊脂 (TSA-YE)
胰蛋白胨 15.5g 大豆蛋白胨 5.0g
NaCl 5.0g 酵母膏 6.5g
瓊脂 15.0g 蒸餾水 1000ml
pH 7.3
66、ATCC 20
蛋白胨 5.0g 酵母膏 15.0g
K2HPO4 3.0g 葡萄糖 2.0g
瓊脂 20.0g 蒸餾水 1000ml
pH 7.3-7.5
67、TY (Yeast Tryptone Agar)
蛋白胨 10.0g 酵母膏 5.0g
葡萄糖 1.0g NaCl 5.0g
瓊脂 20.0g 蒸餾水 1000ml
pH 7.0
68、硫醇(Thiol)培養基 (ATCC 49)
示蛋白胨(Proteose Peptone No.3,Difco) 10.0g 酵母膏 5.0g
葡萄糖 1.0g NaCl 5.0g
硫醇復合物 8.0g 瓊脂 1.0g
p-氨基苯甲酸 0.05g pH 6.9-7.3
69、Czapek'Dox 瓊脂培養基 (ATCC 134)
Czapek'Dox Broth 35.0g 瓊脂 15.0g
蒸餾水 1000ml