22、Pine Block or Pine Sowdust Medium （松木條或松木屑培養基）
（1）、Cut 1021 cm pine biocks and immerse them in 1-2% sucrose solution . Alow the biocks fully absorbing suger solution .Fill the biocks into broad month of bottle . Park the bottle with paper . Autoclave at 20 lbs for 1 hr . (將10ⅹ2ⅹ1厘米的松木條浸泡在1-2%的蔗糖水中。使木條吸足糖水，裝瓶不宜過緊，用紙包好，20磅滅菌1小時。)
（2）、Pine Sawdust Medium
Pine Sawdust (松木屑) 75% Rice bran (米糠) 23-24%
Socrose (蔗糖) 1-2% Water (appropriate)水 （適量）
Autoclave at 20 lbs for 1 hr.(20磅滅菌1小時）
23、Rice Straw. Dung Medium (稻草、馬糞培養基)
Dried horse dung (or cow dung) （干馬糞或牛糞） 65%
Died straw （干稻草） 35% Urea （尿素） 0.5%
Calcium superhosphate （過磷酸鈣）0.7% Gypsum powder（石膏粉） 1%
Ammonium sulphate （硫酸銨） 0.3%
[Note]: Add appropriate water, pile them up and cause them fermentation. （水適量進行堆積發酵）
24、 Straw Rice Bran Medium （稻草、米糠培養基）
Dried straw （干稻草） 75-80% Rice bran （米糠） 20-25%
[Note]：Select
no mouldy straw and cut them about 3 cm in length. Immerse them in
fresh water for 12 hr. Allow the straw fully (the amount of water added
may be decided by kneading with fingers. It is right when a drop of
water appeared between fingers). (
[注]：選無霉稻草，切成長約3厘米的小段，用清水浸泡12小時，使充分吸水，然后傾去水，加米糠和水，水量以用手捏時指間有水珠為宜。)
25、 Rice Medium (米飯培養基)
Fill
10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml water.
Autoclave at 121℃ for 30 minutes (or fill 2g of rice into a 15ⅹ150mm
tube). Add 6 ml of water. Autoclave at 121℃ for 30 minutes.
(取大米10克，入50毫升椎形瓶中，水20-30毫升于15磅滅菌30分鐘即成，或取大米2克，裝入15ⅹ150毫米試管中，加水6毫升，于15磅滅菌30分鐘。)
26、Filter Paper Medium (濾紙條培養基)
(NH4)2SO4 1g KH2PO4 1g
MgSO4.7H2O 0.5g Yeast Extract (酵母膏) 0.1g
Distilled Water (蒸餾水) 1000ml
Strip of filter paper 1ⅹ7 cm (濾紙條 1ⅹ7 厘米)
[Note]：Siddolve
the above ingredients. Distribute the liqid medium into tubes (each
tube contains 1ml of the broth). Autoclave at 121℃ for 30 minutes. Then
put one or two sterilized strips of filter paper into a tube
aseptically.
(將上述溶液分配成溶液后分裝入試管，每管1毫升，15磅滅菌30分鐘。再將滅菌的濾紙條（1條或2條）投入試管中即好使用。)
27、Yeast Extract-Medium Extract Agar I (酵母膏、麥芽膏瓊脂)
Yeast extract (酵母膏) 10g Mall extract (麥芽膏) 10g
Glucose (葡萄糖) 4g Distilled water (蒸餾水) 1000ml
Agar (瓊脂) 20g pH 7.0
適用范圍：紅霉素鏈霉菌
28、Yeast Extract Peptone （酵母膏、蛋白胨瓊脂）
Yeast extract （酵母膏） 1g Multi-peptone（多蛋白胨） 2g
Beef extract （牛肉膏） 1g Glucose （葡萄糖） 10g
Agar （瓊脂） 20g Distilled water （蒸餾水） 1L
pH 7.0
29、Glucose Yeast Extract Starch Agar （葡萄糖、酵母、淀粉瓊脂）
Glucose （葡萄糖） 10g Yeast extract （酵母膏） 10g
Starch （淀粉） 10g NaCl 5g
CaCO3 3g Agar （瓊脂） 20g
Distilled water （蒸餾水） 1L pH 7.0
30、 Wheat Bran Medium （麩皮培養基）
Wheat bran （麩皮） 36g （NH4）2HPO4 10g
K2HPO4 0.2g MgSO4。7H2O 0.1g
Agar （瓊脂） 20g Distilled water （蒸餾水） 1L
pH 7.0
31、Yeast Sporulation Medium (酵母菌產生子囊孢子培養基)
(1) Kleyn Medium
KH2PO4 0.012% K2HPO4 0.02%
Sodium acetate (醋酸鈉) 0.5% Glucose (葡萄糖) 0.062%
NaCl 0.062% Peptone (蛋白胨) 0.25%
Biotin (生物素) 2μg% Agar (瓊脂) 2%
Trace element solution (混合鹽類) 1ml/100ml
[Note]: Trace element solution （混合鹽類溶液）： MgSO4.7H2O 0.4%, NaCl 0.4%, CuSO4.5H2O 0.002%, MnSO4.4H2O 0.2%, FeSO4.4H2O 0.2%
(2) Gorodkowa Medium
Glucose (葡萄糖) 0.1% Peptone (蛋白胨) 1%
NaCl 0.5% Agar (瓊脂) 2%
(3) McClary Medium
Glucose (葡萄糖) 0.1% KCl 0.18%
Yeast juice (酵母汁) 0.25% Sodium actate (醋酸鈉) 0.82%
Agar (瓊脂) 1.5%
32、 BPY Medium (BPY 培養基)
Beef extract (牛肉膏) 5g Prptone (蛋白胨) 10g
NaCl 5g Yeast extract (酵母膏) 5g
Glucose (葡萄糖) 5g Agar (瓊脂) 1-2%
Distilled (蒸餾水) 1000ml pH 7.0
適用范圍：大腸埃希氏菌（大腸桿菌）、惡臭假單胞菌
33、 LB Medium (LB 培養基)
Yeast extact (酵母膏) 5g Peptone (蛋白胨) 10g
NaCl 10g Agar (瓊脂) 1-2%
Distilled water (蒸餾水) 1000ml pH 7.0
適用范圍：大腸埃希氏菌（大腸桿菌）
34、Penssay Broth (Penssay 肉湯)
Beef extract (牛肉膏) 1.5g Yeast extract (酵母膏) 1.5g
Tryptone (胰蛋白胨) 5g NaCl 3.5g
Glucose (葡萄糖) 1g K2HPO4.3H2O 4.8g
Distilled water (蒸餾水) 1000ml pH 7.2
35、Blond Agar (血瓊脂培養基)
Peptone (蛋白胨) 10g Glucose (葡萄糖) 2g
NaCl 5g NaH2PO4 2.5g
Sodium citrate (檸檬酸鈉) 5g Brain infusion (腦浸汁) 800ml
Pig heart infusion (豬心浸) 200ml Sleep Blood (羊血) 10%
pH 7.2
[Note]：Preparation of pig brain and pig heart infusion：
（1） Smash up 100g fresh pig heart （without fat and blood vessel）.Add distilled water up to 1000ml;
（2） Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml;
（3） Keep the two infusions at 50℃ for 1 hr , then boil for 15 minutes;
（4）
Cool and filtrate through gauze .
[注]豬心和豬腦浸汁法：（1）新鮮豬心除去心頭和脂肪，攪碎100克加蒸餾水至1000毫升；（2）新鮮豬腦除去血管，取200克加蒸餾水至1000毫升；（3）50℃保溫1小時，然后煮開15分鐘；（4）冷卻后用紗布過濾備用。
36、 Cellulose﹣decomposing Bacteria Synthetic Medium (纖維細菌合成培養基)
NaCl 6g MgSO4.7H2O 0.1g
KH2PO4 0.5g CaCl2 0.1g
K2HPO4 2.0g Yeast extract (酵母膏) 1.0g
(NH4)2SO4 2.0g Distilled water (蒸餾水) 1000ml
Filter paper strip: one strip (濾紙條：一片) pH 7.0-7.4
[Note]: So;id medium for cellulose-decomposing bacteria: Carboxymethyl Cellulose 2% Agar 2%. （斜面培養基去濾紙條用2%瓊脂及2%羧甲基纖維素鈉 CMCNa ）
37、Clostridium Pasreurianum Synthetic Medium （巴氏梭狀芽孢桿菌合成培養基）
Glucose （葡萄糖） 1% MnSO4 0.001%
KH2PO4 0.05% FeSO4 0.001%
K2HPO4 0.05% Yeast extract（酵母膏） 0.1%
MgSO4.7H2O 0.02% Peptone (蛋白胨) 0.01%
NaCl 0.001% CaCO3 0.5%
Distille water (蒸餾水) 100% pH 7.0
38、Yeast Extract-Medium Extract Agar Ⅱ (酵母膏、麥芽膏瓊脂 Ⅱ)
Yeast extract （酵母膏） 4g Mall extract （麥芽膏） 10g
Glucose （葡萄糖） 4g Agar （瓊脂） 17g
Distilled water （蒸餾） 1000ml pH 7.3
39、Oatemeal Agar （燕麥粉瓊脂）
Oatemeal（infusion）[燕麥粉浸液] 20g Agar （瓊脂） 18%
Trace element solution （跡量鹽溶液）1ml
Distilled water up to （用蒸餾水補充至1000毫升） 1000ml pH 7.2
[Note]：Trace element solution (跡量鹽溶液)：
FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g
ZnSO4.7H2O 0.1g Distilled water (蒸餾水) 100ml
40、Calcium Malate Agar (蘋果酸鈣瓊脂)
Calium malate (蘋果酸鈣) 10g K2HPO4 0.5g
NH4Cl 0.5g Glycerol (甘油) 10g
Agar (瓊脂) 15g Distilled water (蒸餾水) 1000ml
41、Thiobacillus Ferrooxidans Medium (氧化亞鐵硫桿菌培養基)
(NH4)2SO4 0.15g KH2PO4 0.05g
KCl 0.05g MgSO4.7H2O 0.5g
Ca(NO3)2.4H2O 0.01g FeSO4.7H2O 50g
Distilled water (蒸餾水) 1000ml pH 2.0
42、Thiobacillus Thiooxidans Medium (氧化硫硫桿菌培養基)
(NH4)2SO4 0.3g KH2PO4 3-4g
CaCl2 (anhydrous) [無水] 0.25g MgSO4.7H2O 0.5g
FeSO4.7H2O 0.001g Distilled water (蒸餾水) 1000ml
pH 3.5-4