Bruker推出新一代ultrafleXtremeMALDITOF／TOF質譜儀

　　2009年4月27日，布魯克道爾頓公司推出新一代ultrafleXtreme MALDI-TOF/TOF質譜儀，這是市場上唯一的，在TOF和TOF/TOF模式下都能獲得真正的1 kHz采樣速度的儀器，可用于廣泛的研究、臨床、和應用蛋白質組學。

　　布魯克的新型ultrafleXtreme質譜儀，具有1 kHz smartbeam-II激光束技術，可應用于蛋白質組學、生物標志物發現和定量、生物醫藥的質控、組織分子成像和從上到下(Top-Down)的測序。

　　在蛋白質組學研究中，MALDI-TOF/TOF技術是ESI-MS/MS技術強有力的補充手段，這類ESI-MS/MS技術包括：布魯克帶ETD解離技術、高容量的離子阱，和超高分辨的maXis (UHR) ESI-Q-q-TOF系統，在現代的蛋白質分析中，MALDI和ESI技術都需采用，以獲得最高的序列覆蓋度和靈活性。

　　新型ultrafleXtreme合并了幾大發明：布魯克獨一無二的1 kHz smartbeam-II激光技術，最新的離子光學技術。它是唯一的獲得1 kHz高通量的MALDI-TOF/TOF，可用于生物標志物發現和定量;同時，激光束的尺寸非常接近于單細胞的空間分辨，可高效地進行組織分子成像。 LC-MALDI技術也能獲得無與倫比的高分辨率、精確質量和高速的數據采集。Bruker獨一無二的Edmass 蛋白測序能力，可對天然蛋白進行快速和靈敏的蛋白表征，可以測得高至80個殘基的序列，包括直接分析N-端和C-端修飾。

　　ultrafleXtreme集成了業界領先的TOF/TOF技術，包括：

　　新型的1 kHz smartbeam-II激光，對于MS和MS/MS都可以進行快速的數據采集。可進行蛋白組織成像、天然蛋白分析、生物或寡核苷酸的質控，LC-MALDI的蛋白質組分析——所有都可在1 ~ 1000 Hz調制。

　　寬帶質量分辨率高達40,000，可進行精準的蛋白質組學分析，經由Bruker獨特的PAN技術，可獲得跨越寬闊質量范圍的高分辨率，而不僅僅是針對某一小段質量數。

　　新型的FlashDetector檢測器合并了4 GHz的數字轉換器，可獲得40,000分辨率和 1 ppm質量準確度，可使結果更為確信。

　　BrukerZL的smartbeam技術，已被公認為是激光技術中最多樣化的MALDI成像技術。ultrafleXtreme如今可獲得小至10 μm直徑的激光聚焦，獲得高的空間分辨率，沒有像素重合。重要的是，在如此小的激光束直徑下，還可以獲得出眾的譜圖質量和信號強度。

　　新型獨特的激光-紅外自清洗MALDI源，可獲得長時間穩定的高性能。非常長的MALDI激光束壽命，加上自動的源清洗功能，可獲得最高的運行時間，降低了維護的成本。

　　Bruker全面的軟件平臺上，集成了各種有效的數據處理方式：

　　ProteinScape 2.0確保獲得定量研究的確定性高的信息：不管是從Label-free(非標記)的方法，或是ICPL-4 plex，SILAC，iTRAQ-8plex，還是其它用戶自定義的標記技術。它集成了大規模蛋白質組學研究的多種數據組，并根據標準的、公開的原則產生報告。

　　BioTools 3.2是市場上領先的生物信息學平臺，現在可對重組蛋白進行top-down蛋白測序，比如對于生物治療和生物相似物的表征和質量控制。

　　flexImaging 2.1是基于MALDI 組織分子成像平臺上的生物標志物發現的黃金標準，全面兼容于臨床組織學。

　　布魯克.道爾頓公司的執行副總裁 Ian Sanders博士評論說：“ ultrafleXtreme具有當今MALDI-TOF/TOF質譜無與倫比的靈活性和性能。研究者們可以利用它在蛋白質組學和MALDI成像方面獲得更新的進步，即使在很短時間以前，研究者們還僅是夢想能在如此寬的范圍內獲得這樣的數據質量。”

　　ultrafleXtreme的性能將要在5月31日，2009年第57屆ASMS上在Bruker的用戶會上展示。

　　Bruker Daltonics today launches its next-generation ultrafleXtreme MALDI-TOF/TOF mass spectrometer, the only instrument combining true 1 kHz speed in both TOF and TOF/TOF modes with ultra-high performance and extreme flexibility for a broad variety of complementary research, clinical and applied proteomics applications.

　　Bruker's Novel ultrafleXtreme(TM) with 1 kHz smartbeam-II(TM) Laser Technology Redefines MALDI-TOF/TOF Performance for Proteomics, Biomarker Discovery and Quantitation, Biopharma QC, Tissue Molecular Imaging and Top-Down Sequencing (Photo: Business Wire)

　　In proteomics, MALDI-TOF/TOF is highly complementary to ESI-MS/MS with Bruker's latest high-capacity ion traps with ETD, and to ultra-high resolution (UHR) ESI-Q-q-TOF maXis systems, as both MALDI and ESI are required for highest coverage and flexibility in modern protein analysis.

　　The new ultrafleXtreme incorporates key inventions, Bruker's proprietary smartbeam-II laser technology at 1 kHz, and the latest ion optics innovations. It is the only MALDI-TOF/TOF instrument that truly enables high-throughput 1 kHz biomarker discovery and quantitation, as well as fast, high-performance tissue molecular imaging with a laser spot size close to single cell spatial resolution. LC-MALDI can be performed with unmatched mass resolution, mass accuracy and data acquisition speed. Bruker's unique Edmass protein sequencing capabilities allow very rapid and sensitive intact protein characterization and sequencing with read lengths up to and beyond 80 residues, and with direct analysis of N- and C- terminal modifications.

　　Industry-leading TOF/TOF technology is incorporated into the ultrafleXtreme for unprecedented capabilities:

　　The novel full 1 kHz smartbeam-II laser enables ultra-high data acquisition speed in both MS and MS/MS at full systems performance. The well-established proprietary smartbeam laser provides unprecedented analytical and matrix flexibility in workflows from protein tissue imaging, intact proteins analysis, biologics or oligo QC, to LC-MALDI proteomics - all fully enabled at 1-1000 Hz repetition rates.

　　Broadband mass resolving power up to 40,000 enables precision proteomics via Bruker's unique PAN technology for highest mass resolution across a very broad mass range, not just at a selected optimum.

　　The novel FlashDetector TM combined with a new 4 GHz digitizer and latest advances in electronics provide unmatched mass resolving power up to 40,000 and 1 ppm mass accuracy for highest confidence.

　　Bruker's patented smartbeam technology is already widely accepted as the most viable MALDI imaging laser technology. The ultrafleXtreme now enables laser focus diameters down to 10 μm for high spatial resolution imaging without pixel overlap. Importantly, outstanding spectral quality and signal intensity are maintained at even the smallest laser beam diameters.

　　The novel and unique laser-irradiation self-cleaning MALDI Perpetual I on S ource ensures robust, long-term highest-performance operation. Very long MALDI laser lifetime in combination with automated source cleaning in just minutes leads to high uptime and low maintenance costs.

　　Efficient data retrieval and interpretation is provided by fully integrated software platforms designed, progressed and supported by Bruker with leading proteomics experts, for:

　　ProteinScape 2.0 ensures confident information from quantitative studies using either label-free or ICPL - 4plex, SILAC, iTRAQ-8plex or user-defined label technologies, integrates multiple datasets across large proteomic studies and generates reports in accordance to standard publication guidelines.

　　BioTools 3.2 is the market-leading bioinformatics platform, now extended to top-down protein sequencing for recombinant protein work, e.g. in biotherapeutics and biosimilars characterization and QC.

　　flexImaging 2.1 is the true gold standard in biomarker discovery based on MALDI tissue molecular imaging and is fully compatible with clinical histology .

　　Bruker Daltonics Executive Vice President Dr. Ian Sanders commented: "Its incredible flexibility and unparalleled performance make the truly innovative ultrafleXtreme the definitive MALDI-TOF/TOF today. Researchers are enabled for new achievements in proteomics and MALDI imaging, while achieving result quality across the board that would have been undreamed of even a short time ago."

　　The capabilities of the new ultrafleXtreme will be highlighted at Bruker's Annual Users Meeting on Sunday, May 31 st , 2009 before the 57 th ASMS Conference on Mass Spectrometry in Philadelphia, PA. For more information on this event, please visit www.bdal.com/asms2009 . For further product information please visit www.bdal.com/ultrafleXtreme .

　　ultrafleXtreme, ProteinScape, PAN, smartbeam , FlashDetector, MALDI Perpetual, BioTools, ProteinScape, FlexImaging are trademarks of Bruker Daltonik GmbH. ICPL is a trademark of TopLab GmbH, and iTRAQ is a trademark of Applied Biosystems.