?SCX and high pH reversed phase fractionation are both orthogonal to low pH C18 LC separation
?Strong cation exchange (SCX) requires sample desalting after fractionation
Reproducibly Identify More TMT-labeled Peptides
?Significantly increase the number of proteins identified and quantified
?The percentage and number of peptides observed in only 1 fraction are within 10% between runs
Two New Peptide Quantitation Assays
Colorimetric peptide assay is more sensitive than BCA
Two New Peptide Quantitation Assays
Thermo Poster Note: Quantitative Peptide Assays for Mass Spectrometry Applications; Haney P. et al
Peptide Quantitation Improves MS Reproducibility
Thermo Poster Note 64604: Quantitative peptide assay for optimized and reproducible sample preparations for mass spectrometry applications; Jiang X. et al
Peptide Assays Provide Consistent Quantification
“We really like those peptide quant kits. We find that peptide mass quantitation is directly relevant for determining the amount of analyte we use for LC-MS/MS analyses, phosphopeptide enrichments, and for TMT labeling.
Instrument Configuration
Introducing A New Powerful Combination
Unlock the performance of your MS with incredibly easy chromatography
[EASY-nLC? 1200 HPLC System]
?Industry leading 1200 bar system pressure
?Improved system robustness and easier maintenance
?Temperature control of EASY-Spray columns
?Effortless ultra-high performance for every user, every time
[75cm EASY-Spray? Column]
?Increased peak capacity
?Even more identifications
?Excellent retention time consistency
?Improved quantitative reproducibility
Extend The Performance Of Your MS with 75cm Columns
Improved Protein Identifications
?Peak capacity exceeding 800.
?Deeper proteome coverage with a consistent 5700 protein groups per run.
?9% increase in protein groups over 3 combined runs compared to 50 cm column.
?Identify more proteins per hour – comparable identifications in 120 minutes to a 50 cm column in 240 minutes.
Increased Quantitative Reproducibility
?Less run-to-run variability 91% identification overlap run to run
? More quantifiable proteins 50% increase in the number of quantifiable proteins with CVs <5%