Promega在ASMS2009上推出鑒定蛋白質的新途徑

　　Promega利用ProteaseMAX表面活性劑推出了一項創新簡化的膠內酶切技術。該項技術給分析人員提供了以下幾點好處：

• 　　節省時間：整個膠內酶切過程只需一個小時的時間
• 　　省去了萃取步驟：蛋白質分解和肽段回收一步完成。
• 　　更多的數據：該分解技術保持了原有肽段的信息。

　　除了ProteaseMAX 表面活性劑意外，Promega還推出了以下幾款新產品：

• 　　固定化胰蛋白酶
• 　　胰凝乳蛋白酶測序級
• 　　Lys-C測序級

　　固定化胰蛋白酶提高了純化蛋白質的分離速度。蛋白酶切過程只需短短的30分鐘，可適用于多種濃度的蛋白質。胰凝乳蛋白酶和Lys-C既能單獨使用也可以結合其它蛋白酶，通過肽質量指紋圖譜或MS/MS譜圖匹配的方法來識別蛋白質分解產生的肽譜，從而進行蛋白質鑒定。該兩項蛋白酶都適用于在溶液或膠內催化蛋白質分解。

參考英文原文

Identify proteins you could not identify before using new products from Promega

Promega Corp.

Promega introduces an innovative and simplified in-gel digestion procedure utilizing ProteaseMAX Surfactant. This new procedure offers the researcher multiple benefits:

? Save time: Digestions complete in only one hour.

? Eliminate gel extraction steps: Protein digestion and peptide recovery is complete in a single step.

? More data: Recover longer peptides which tend to remain in the gel in conventional in-gel digestion protocols.

In addition to ProteaseMAX Surfactant, Promega now offers the following new products:

? Immobilized Trypsin

? Chymotrypsin Sequencing Grade

? Lys-C Sequencing Grade

Immobilized Trypsin offers fast digestion of purified proteins or complex mixtures. Digestions are complete in as little as 30 minutes and the protocol is easily scalable to accommodate multiple protein concentrations. Chymotrypsin and Lys-C can both be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. Both proteases are suitable for digestion reactions in-solution or in-gel.

Request your free sample of Immobilized Trypsin, just visit us at ASMS booth #63 to find out how.