Ruedi小組用QTRAP開發出全球首個定量蛋白質組數據庫

蘇黎世理工學院借助AB SCIEX的QTRAP技術研發成功全球首個蛋白質組學MRM Atlas

基于質譜的在線數據庫加速蛋白質分析

　　福斯特市，加利福尼亞—2010年10月4日，生命科學分析技術的全球領導者AB SCIEX公司，今天宣布瑞士聯邦理工學院(蘇黎世理工學院，ETH)與西雅圖的華盛頓大學系統生物研究所合作，使用新一代QTRAP質譜技術測定了近20,000個人體蛋白質，首創全球第一個人類多反應監測(MRM) Atlas數據庫，MRMAtlas是一個能夠實現數據共享的在線數據庫。全球的科學家都可以使用atlas數據庫，來顯著提高生物標記物研究、基于蛋白的藥物發現和基礎生物及生物醫學研究的效率。MRM Atlas提供了簡單、認證的方法，可以將蛋白質組學研究拓展到前所未有的領域。該項目由Ruedi Aebersold 博士領導的ETH科學家完成，Ruedi 博士將出席11月9日播出的一個技術介紹類的現場網播節目，向大家介紹MRMAtlas。

Prof. Ruedi Aebersold

　　MRMAtlas是一個全新的數據庫，提供了大量基于質譜的人類蛋白質組信息，使得研究者能夠盡可能縮短冗長并且耗時的開發時間。許多科學家都將MRM視為現有免疫測試的改進方法，現有方法包括蛋白質印跡法(Western Blotting)和酶聯免疫吸附測定(ELISA)等，這兩種方法均使用抗體。用免疫測試是一種非常昂貴且耗時的開發方法，還有一系列特異性的問題。全新的MRM數據庫與大型現成的特定蛋白質抗體數據庫相似;區別在于，成百上千的蛋白質和多肽可用簡單的質譜方法檢測和定量，而不需要交叉反應。

　　MRMAtlas數據庫的開發依賴于QTRAP技術，Qtrap獨有的技術可在一次實驗中結合兩種能力——三重四極桿系統的定量MRM能力;先進的線性離子阱的蛋白質鑒定及確證能力。這給研究者提供了每個樣本的清晰圖像，減小了不確定性，滿足了蘇黎世理工學院科學家的要求。AB SCIEX QTRAP系統業界領先的靈敏度同樣意味著，科學家可以開發出蛋白質組中低豐度蛋白測定的方法。使用QTRAP分析時，蘇黎世理工學院的科學家開發了這一全面的MRM數據庫，并可獲得100個多肽/小時的空前速度分析合成多肽。

 

　　Ruedi Aebersold博士，教授，瑞士聯邦理工學院生物技術研究所

　　“MRM方法將改變全世界的蛋白質組學研究。MRMAtlas是一種先進的蛋白質分析工具，這項由具有現身精神的科學家團隊完成的工作是一個重要的里程碑，將把我們對人類蛋白質組分析的理解提高到一個新的層次。如果沒有QTRAP技術，我們是不可能這么快速而高效地開發出MRMAtlas的。”

 

　　Andy Boorn，首席運營官，AB SCIEX

　　“AB SCIEX QTRAP系統幫助我們創建了MRMAtlas，而MRMAtlas也將會展示出質譜在蛋白質組分析方面的真正能力。科學家們從現在起將能夠同時分析人體內數量巨大的蛋白質，這對于生物標記物研究和最終的個性化治療將產生重大影響。QTRAP系統本質上是最好的三重四極桿，同時具有附加的和獨特的能力。正如里程碑式的MRMAtlas項目一樣，QTRAP將繼續幫助科學家們取得重要的科學成果。”

 

　　關于AB SCIEX

　　AB SCIEX是生命科學分析技術開發領域的全球領先企業，幫助科學家應對復雜的科學挑戰。公司提供科學儀器、軟件和服務，用于發現新藥、促進醫學科學發展、保護食品供應鏈和環境。AB SCIEX的技術解決方案結合了最高的性能和最高的穩定性，確保我們的用戶進行科學發現、可靠地傳遞結果和提高生命質量。AB SCIEX擁有近30年的創新歷史，在市場中一直出于領先地位，其前身為美國應用生物系統公司(Applied Biosystems)和美迪希(MDS)實驗儀器公司的合資企業。2010年1月30 日，AB SCIEX作為丹納赫集團的子公司開始運營。更多資料請訪問官網：www.absciex.com。

　　http://www.mrmatlas.org/

　　QTRAP技術概覽

 

　　本網收錄相關儀器：

　　3200 Qtrap 四極桿-線性阱雜交質譜儀

 　　4000 Qtrap四極桿-線性阱雜交質譜儀

　　QTRAP5500 四極桿-線性阱雜交質譜儀

ETH Zurich Develops the World's First Proteomics MRM Atlas with QTRAP Technology from AB SCIEX

Online database of mass spectrometry-based assays to accelerate protein analysis

　　FOSTER CITY, Calif. – October 4, 2010 – AB SCIEX, a global leader in life science analytical technologies, today announced that scientists at the Swiss Federal Institute of Technology (ETH Zurich), in collaboration with the Institute for Systems Biology in Seattle, WA, used next-generation QTRAP mass spectrometry technology to create assays for approximately 20,000 human proteins, generating the world’s first human Multiple Reaction Monitoring (MRM) Atlas, an online database that will be made publicly available. Scientists around the world can use this atlas to significantly advance biomarker research, protein-based drug development and fundamental biological and biomedical research. The MRM Atlas provides simple, validated assays expanding into never-before-seen areas of the proteome. This project involved ETH scientists led by Dr. Ruedi Aebersold, Ph.D., who will be featured in a November 9th live webcast of a technical presentation about the MRMAtlas.

　　The MRMAtlas is a new database that provides mass spectrometry-based assays to a large proportion of the human proteome, enabling researchers to minimize the tedious and time-consuming task of assay development. Many scientists see MRM assays as an improvement over current immunoassays, such as Western Blotting and ELISA, which use antibodies. Immunoassays can be very costly and time-consuming to develop and can have issues with specificity. The new database of MRM assays is similar to a large library of ready-to-go antibodies to specific proteins, but the difference is that hundreds of proteins and peptides can be detected and quantified in a simple mass spectrometry assay and without the issues of cross reactivity.

　　The development of the MRMAtlas was dependent on QTRAP technology, which uniquely combines in a single analysis the quantitative MRM capabilities of normal triple quadrupole systems, with advanced linear ion trap capabilities for protein identification and confirmation. This gives researchers a clearer picture of each assay and eliminates ambiguity, meeting the requirements of the scientists at ETH Zurich. The industry-leading sensitivity of the AB SCIEX QTRAP systems also means the scientists were able to develop assays to low abundant proteins in the proteome. Using synthetic peptides for the QTRAP technology to analyze, the scientists at ETH developed this comprehensive library of MRM assays with unprecedented speed of 100 peptides per hour.

　　Ruedi Aebersold, Ph.D., Professor, Institute of Biotechnology at the Swiss Federal Institute of Technology

　　“MRM assays are set to change the world of proteomics. The MRMAtlas is a tool for advancing protein analysis. This accomplishment by a dedicated group of scientists is an important milestone and will improve our understanding of the analysis of the human proteome. We could not have developed the MRMAtlas as quickly and productively without QTRAP technology.”

　　Andy Boorn, Chief Operating Officer, AB SCIEX

　　“The adoption of AB SCIEX QTRAP systems to help create the MRMAtlas will unlock the real power of mass spectrometry for proteomics analysis. Scientists will now be able to analyze huge numbers of proteins in the human body all at the same time, which has significant ramifications for biomarker research and ultimately personalized medicine. The QTRAP systems are essentially the best triple quadrupoles available but with additional, unique capabilities. They continue to help deliver significant scientific results, such as this landmark MRMAtlas project.”

　　About AB SCIEX

　　AB SCIEX is a global leader in the development of life science analytical technologies that help answer complex scientific challenges. The company provides scientific instrumentation, software and services used to discover new drugs, advance medical science and protect the food supply and the environment. AB SCIEX technology solutions combine the highest performance with the highest reliability to enable our customers to fuel scientific discovery, deliver results with confidence and improve the quality of life. The company has a more than 20-year history of innovation and market leadership as the former Applied Biosystems/MDS Analytical Technologies joint venture. For more information about AB SCIEX, go to www.absciex.com. Follow AB SCIEX on Twitter @ABSCIEX and on Facebook.