MS MEDIUM FOR ARABIDOPSIS
To 990 ml H2O add:
Sucrose ........... 10.0 g
MOPS .............. 0.5 g
Agar .............. 8.0 g
Adjust pH to 5.7 with 1 M KOH.
Autoclave.
When cooled add sterile stock solutions of:
KNO3............... 20.0 ml 940 mM (95.00 g/L)
NH4NO3............. 20.0 ml 1000 mM (80.04 g/L)
KH2PO4............. 12.5 ml 100 mM (13.60 g/L)
MgSO47H2O.......... 15.0 ml 100 mM (24.65 g/L)
CaCl27H2O.......... 10.0 ml 300 mM (44.00 g/L)
Glycine............ 2.67 ml 10 mM
Nicotinate......... 0.4 ml 10 mM
Thiamin............ 0.02 ml 10 mM
PyridoxineHCl...... 0.24 ml 10 mM
Myoinositol........ 5.5 ml 100 mM
FeSO47H2O ......... 1.0 ml (2.780 g/100 ml)
Na2EDTA ........... 1.0 ml 100 mM (3.720 g/100 ml)
MnSO44H2O.......... 1.0 ml 100 mM (1.690 g/100 ml)
ZnSO44H2O.......... 3.0 ml 10 mM (0.288 g/100 ml)
H3BO3 ............. 1.0 ml 100 mM (0.618 g/100 ml)
KI................. 0.5 ml 10 mM (0.166 g/100 ml)
CuSO45H2O ......... 0.01 ml 10 mM (0.170 g/100 ml)
Na2MoO42H2O ....... 0.1 ml 10 mM (0.242 g/100 ml)
CoCl26H2O ......... 0.01 ml 10 mM (0.238 g/100 ml)B5 MEDIUM FOR ARABIDOPSISTo 990 ml H2O add:
Sucrose ........... 20.0 g
MOPS .............. 0.5 g
Agar .............. 8.0 g
Adjust pH to 5.7 with 1 M KOH.
Autoclave.
When cooled add sterile stock solutions of:
KNO3............... 26.3 ml 940 mM (95.00 g/L)
NaH2PO4H2O......... 11.0 ml 100 mM
MgSO47H2O.......... 10.0 ml 100 mM (24.65 g/L)
(NH4)2SO3.......... 10.0 ml 100 mM
CaCl27H2O.......... 3.3 ml 300 mM (44.00 g/L)
Myoinositol........ 5.5 ml 100 mM
Thiamine........... 3.0 ml 10 mM
Nicotinate......... 0.8 ml 10 mM
PyridoxineHCl...... 0.48 ml 10 mM
10X Micronutrients. 10.0 ml (see below)
Sequestrene 330 Fe. 10.0 ml (0.28 g/100 ml)
For Callus Inducing Medium (CIM) add:
2,4-D..................... 0.5 ml
Kinetin................... 0.05 ml
For Shoot Inducing Medium (SIM) add:
IAA....................... 0.15 ml
6-dimethylaminopurine..... 5.0 ml10X Micronutrients (per 100 ml)
MnSO44H2O.......... 0.6 ml 100 mM (1.690 g/100 ml)
ZnSO44H2O.......... 10.07 ml 10 mM (0.288 g/100 ml)
H3BO3 ............. 4.9 ml 100 mM (0.618 g/100 ml)
KI................. 4.5 ml 10 mM (0.166 g/100 ml)
CuSO45H2O ......... 2.3 ml 10 mM (0.170 g/100 ml)
Na2MoO42H2O ....... 1.0 ml 10 mM (0.242 g/100 ml)
CoCl26H2O ......... 1.0 ml 10 mM (0.238 g/100 ml)
