實驗概要
The following protocol provides a method of immunofluorescence of rabbit anti-murine VEGF by Peprotech.
實驗步驟
The following protocol used normal murine kidney tissue with an autostainer.
1. Deparaffinize and rehydrate the tissue section.
2. Perform heat-induced antigen retrieval with a high pH buffer for 20 minutes. Allow the slide to cool for 20 minutes.
3. Wash the slide for 5 minutes (TBS/Tween 20).
4. Use the following autostainer protocol:
1) Incubate the tissue section for 1 hour at room temperature with Rabbit Anti-Murine VEGF at 2.5 μg/mL.
2) Wash the slide.
3) Incubate the tissue section with a fluorescent conjugated secondary antibody for 30 minutes at room temperature.
4) Wash the slide.
5. Counterstain the tissue section with DAPI.