質譜學報紀念楊芃原教授專輯

楊芃原教授是我國質譜領域的先鋒科學家，他充沛的探索熱情和無私的奉獻精神影響了一代又一代質譜人。

楊芃原教授熱心中文期刊發展事業，他曾擔任《質譜學報》第六、七、八、九、十屆副主編，還親自為學報策劃“質譜儀器研制專輯”。

為傳承楊芃原教授的學術精神，《質譜學報》編輯部于2026年5月出版了“紀念楊芃原教授專輯”。

感謝執行主編周振教授、陸豪杰教授在專輯的策劃、組約稿件等方面給予的幫助，感謝各位老師為該專輯提供優質稿件。

綜述

化學酶法標記整合質譜技術在蛋白質糖基化分析中的研究進展

趙晨陽,李中華,陸豪杰

DOI: 10.7538/zpxb.2025.0154

This review highlighted recent advances in chemoenzymatic labeling-assisted mass spectrometry for glycoproteomics, emphasizing innovative strategies that integrate enzymatic specificity with chemical versatility to enable selective enrichment, site-resolved identification, and high-throughput analysis of complex protein glycosylation networks.

基于質譜的臨床體液糖蛋白質組學研究進展

蔡飛,令穎穎,李玲,申靖,張勇

DOI: 10.7538/zpxb.2025.0155

This review summarized recent advances in mass spectrometry-based glycoproteomics of human body fluids, highlighting disease-specific glycosylation alterations and their potential as biomarkers for clinical translation.

黏蛋白型O-糖基化蛋白質組學分析進展

劉兆亮, 于永亮, 葉明亮

DOI: 10.7538/zpxb.2025.0156

This review systematically summarized recent advances in mucin-type glycopeptide enrichment—including novel chromatographic materials, O-glycoprotease-based techniques, and bioorthogonal approaches—and mass spectrometry (MS)-based database search strategies. These advances enable quick comprehension of cutting-edge progress and provides valuable insights for developing innovative methodologies.

基于質譜的單細胞蛋白質組學研究進展

付航, 王璐璐, 翟琳輝, 譚敏佳

DOI: 10.7538/zpxb.2025.0150

This review comprehensively summarized advances in mass spectrometry-based single-cell proteomics, highlighting innovations in automated isolation, nanoliter-scale sample preparation, and data analysis. It further explored emerging spatial proteomics technologies and clinical applications, providing critical insights into future multi-omics integration and standardization.

生物組織完整蛋白原位電離與空間組學質譜技術的進展和挑戰

楊治渝, 楊婷婷, 王娜, 岳磊

DOI: 10.7538/zpxb.2025.0169

This work highlighted in situ mass spectrometry strategies for intact protein analysis by integrating in situ sampling and ionization with gas-phase separation and top-down mass spectrometry. The combined IMS-top-down-MSI approach enables structural characterization of membrane proteins, protein complexes, and low-abundance proteins with spatial resolution.

基于質譜的免疫肽組學在腫瘤新抗原鑒定中的研究進展

楊雨岱, 余利巖, 徐平, 張振鵬

DOI: 10.7538/zpxb.2025.0148

This review summarized recent methodological advances in mass spectrometry (MS)-based immunopeptidomics for tumor neoantigen identification, highlighting innovations in sample preparation, data acquisition, and database construction, and emphasizing how integrated multi-omics and advanced MS technologies enable more reliable and experimentally grounded neoantigen discovery.

人工智能助力蛋白質翻譯后修飾的質譜解析及其臨床應用

謝欣然, 楊曉霞, 申華莉, 林靈

DOI: 10.7538/zpxb.2025.0119

This study outlined how artificial intelligence (AI) bridges key technical challenges in post-translational modification (PTM) research to enable clinical translation. The analytical bottlenecks were summarized, including low sensitivity and coverage, structural heterogeneity, and site ambiguity, as exemplified in PTMs such as phosphorylation, glycosylation, acetylation, and lactylation. And the corresponding AI-driven computational strategies were highlighted, encompassing deep learning models, algorithm development, and dedicated PTM data-processing pipelines. The resulting translational outcomes were illustrated, including advances in cancer diagnosis, mechanistic dissection of disease pathways, and the discovery of novel therapeutic targets.

腫瘤與非經典蛋白：從“暗蛋白質組”到診療新策略

范子鴻, 王蘇杰, 何慶瑜, 張靜

DOI: 10.7538/zpxb.2025.0139

This study systematically illustrated the research framework of the dark proteome, delineating the biological origins of noncanonical proteins, the multi-omics technologies for their discovery, their multifaceted roles in tumor biology, and their clinical translational potential.

人參屬植物藥效成分的直接質譜分析研究進展

胡莘如, 劉春穎, 眭沁雪, 張小平, 陳煥文

DOI: 10.7538/zpxb.2025.0144

The review summarized direct ionization techniques (e.g., DART, EESI) coupled with mass spectrometry for rapid, in situ ginseng analysis within 1-60 s without sample pretreatment. This method enables high-throughput, real-time characterization of bioactive compounds, thereby driving advancements in traditional herbal medicine quality control and pharmacological research.

研究論文

四極桿離子碰撞誘導解離系統的研制及其參數優化

朱晨鑫, 劉凱, 楊芃原, 申華莉

DOI: 10.7538/zpxb.2025.0122

This work innovatively developed a dual-mode (transient/static voltage) CID system on a self-built platform, establishing a systematic parameter optimization workflow. The static voltage mode doubled MS/MS signal intensity and enabled closed-loop optimization, thus enhancing domestic instrument capabilities and providing a new strategy for high-performance tandem mass spectrometry.

同軸反射式微型飛行時間質譜儀的研制

楊棟, 劉瑞東, 吳向坤, 李靜, 侯可勇

DOI: 10.7538/zpxb.2025.0149

In this work, a coaxial reflection miniaturized time-of-flight mass analyzer was designed. Its overall performance and related influencing factors were optimized, enabling the detection of copper, iron, aluminum, titanium, and nickel.

超低溫離子阱-紅外光解離光譜-飛行時間質譜的研制及水合離子團簇的結構和溫度依賴性研究

池超賢, 王柏純, 金佳曄, 王冠軍, 周鳴飛,

徐福興, 丁傳凡

DOI: 10.7538/zpxb.2025.0180

A home-built temperature-controlled cryogenic ion trap mass spectrometer coupled with IRPD spectroscopy was used to probe hydrated metal ion clusters and reveal how temperature alters isomer populations and hydrogen-bonding motifs.

大氣壓氣動霧化-飛行時間質譜儀研制及應用

張振元, 蔣吉春, 樊志剛, 任美慧, 黃天浩,花磊, 李海洋

DOI: 10.7538/zpxb.2025.0162

In this study, a TOF-MS equipped with a high-efficiency octopole atmospheric pressure interface was developed. It provides high sensitivity across a broad m/z range of 15-650 and a LOD of 0.3 μg/L for reserpine. The instrument holds significant potential for microdroplet research and in situ characterization of battery electrolytes when coupled with pneumatic spray ionization.

基于timsTOF Pro2的非標記單細胞蛋白質組學技術優化

陳麗穎, 范林園, 張躍飛, 黃玉瀅, 李軍,

徐寧, 任艷, 劉斯奇

DOI: 10.7538/zpxb.2025.0164

This study optimized a label-free single-cell proteomics workflow using a timsTOF Pro2 mass spectrometry. Through systematic optimization of sample preparation, LC-MS parameters, and DIA settings, deep proteome coverage (~2 459 proteins/cell) with high reproducibility was achieved. This study offers a cost-effective strategy for laboratories equipped with comparable instrumentation.

面向親和富集-質譜分析通用需求的生物信息學分析平臺

張騫寧, 何岸, 李元, 柯彌, 田瑞軍

DOI: 10.7538/zpxb.2026.0028

APMSflow is an integrated R pipeline that transforms complicated AP-MS data processing into a user-friendly workflow. By removing the need for extensive custom coding, it uniquely unifies steps from quality control through to interaction network prediction, thereby streamlining protein-protein interaction analysis for experimental researchers.

棕櫚酸誘導HepG2細胞脂毒性模型的深度蛋白質組學

郭佳鳴, 陳光云, 謝文凡, 徐亦陽, 王宇秋,王詩盛, 王賀瑤, 朱洪文, 周虎

DOI: 10.7538/zpxb.2025.0158

This study presented a high-depth proteomic profile of palmitic acid-induced lipotoxicity in HepG2 cells. By integrating three sample preparation methods, the robust protein alterations were identified and the novel mechanisms involving transcriptional regulation, organelle dysfunction, and calcium homeostasis were revealed.

二維離子遷移譜串聯質譜用于探索蛋白質結構多樣性

吳凱群, 雷永琪, 王陳璐, 李俊暉, 俞建成,唐科奇

DOI: 10.7538/zpxb.2025.0141

Multiple IMS peaks can be observed in the IMS spectra for myoglobin ions with +12 to +14 charge states, while multiple FAIMS peaks can be observed in the FAIMS spectra for myoglobin ions with +15 to +25 charge states. This indicated that the gas phase ion separations in FAIMS and IMS are highly orthogonal.

高脂血癥患者血脂凈化治療的血清蛋白質組學研究

徐東, 楊葉曉青, 劉容, 胡中瀚, 呂定豐,唐科奇

DOI: 10.7538/zpxb.2025.0143

LC-MS/MS combined with data-independent acquisition analysis was used to explore the effect of lipoprotein apheresis in patients with hyperlipidemia. Differential expression and bioinformatic analyses were further performed to screen for the potential target proteins which may directly or indirectly prevent or mitigate the progression of hyperlipidemia.

基于質子轉移反應質譜快速分析紡織品中揮發性有機物

李丹丹, 何智豪, 李成林, 江小琴, 段憶翔,趙忠俊

DOI: 10.7538/zpxb.2025.0140

This study developed a rapid PTR-MS method for VOCs screening in textiles, eliminating sample preparation. It achieves detection within one minute per sample and low detection limits, enabling high-throughput analysis for large-scale quality control.

基于激光燒蝕碳纖維離子化質譜法快速鑒別化妝品包裝材料

鄭世博, 鄭榮, 孟茜, 戴維, 蘇越,郭寅龍

DOI: 10.7538/zpxb.2025.0146

This study introduced a rapid, in situ LACFI-MS method for authenticating cosmetic packaging. It directly analyzes surface chemistry without sample pretreatment, identifying stable fingerprints for genuine products and disordered signals for counterfeits, enabling efficient discrimination and potential source tracing.

甲醇介導的多膜集成過濾裝置用于多組學樣品的快速制備

閆帥, 崔春萍, 秦偉捷, 張萬軍

DOI: 10.7538/zpxb.2025.0137

In this study, a method of methanol-mediated multi-Omics via filter (3M-Filter) was introduced, which enables rapid, parallel preparation of proteomics and metabolomics samples within 30 min. This unified workflow provides high-depth and highly reproducible multi-omics coverage from diverse and low-input samples, facilitating robust LC-MS analysis.