DNA標記

DNA標記（主要內容如下）

•   DNA Labeling by Nick Translation

•   Random Primed Labeling

•   End-Labeling

•   Purification of Labeled DNA

•   Non-isotopic Labeling

•   Others

DNA Labeling by Nick Translation

§         DNA Labeling by Nick Translation (Ambros Lab)

§         Nick Translation (Worthington)

§         Nick Translation

§         Nick Translation (Promega)

§         Nick Translation (Life Technologies)

Random Primed Labeling

·         Random Primer Labeling (Karl Clark)
Prepare radioactively labeled DNA strands for the detection of target DNA or RNA sequences in usr/localious applications including Southern and northern blot hybridization. Detailed protocol for labeling, purification and determination of incorporation

·         Random Primed Labelling with P32 (Molecular Genetics Network Lab)

·         Random Primer Labeling (Worthington)

·         Random Primer Labeling (Crawford Lab)

·         Random primer labeling (Amersham Pharmacia)

·         Random Primer  DNA Labeling (Life Technologies)

·         Random Primer DNA labeling (Fermentas)
Labeling of DNA by random oligonucleotide-primed synthesis

·         Random Hexamer Labeling of cDNA Probes (PMCI Research)

End-Labeling

·         5'-End Oligo Fluorescence Labeling (Amersham Pharmacia)
Labeling of  oligonucleotides with a single fluorescein residue at the 5' end

·         5' end labeling of oligonucleotides with gamma-³²P-ATP (Petra Klaff)
Labeling and purification method in great detail

·         3'-end Labeling (Promega)

·         DNA Labeling using Klenow Fragment I (Lazo Lab)

·         End-labeling of DNA fragments
Single end or double ends, can also be used for RNA

·         Labeling of Oligonucleotides (PMCI Research)
Label oligo with polynucleotide kinase. 

·         Alkaline phosphatase labeling of oligos (Promega)
Provides useful guide for preparing phosphatase conjugated oligos

·         Kinase End-Labeling of DNA (Roe, OU)

·         Endlabeling of DNA fragments with Klenow polymerase (Molecular Genetics Network Lab)

·         5'-end Labeling (Amersham Pharmacia)

·         Fill in Labeling of DNA (Mike A. Dyer)

·         5'-End Labeling (Fermentas)
T4 polynucleotide kinase  labeling (ds and ss DNA or RNA; protruding, recessed and blunt 5'-ends) for (1)end labeling of DNA or RNA probes in DNA hybridization and DNA footprinting experiments, etc; (2)obtaining labeled molecular weight markers;(3)obtaining labeled molecular weight markers; (4)labelling of primers for sequencing; (5)addition of 5'-phosphates to oligonucleotides to allow subsequent ligation.

·         Oligonucleotide labeling with ³²P (Hahn Lab)
T4 polynucleotide kinase labeling 

Purification of Labeled DNA

§         5' end labeling of oligonucleotides and purification (Petra Klaff)
Labeling and purification method (ethanol and CTAB in great detail

§         Oligo Purification on Acrylamide Gels (Botwell Lab)
Purification of labeled oligos

·         Sephadex G-50 Spin Column (gopher://iubio.bio.indiana.edu/)
For purifying labeled probe

·         Spin Column chromatography (Schimenti Lab)
Used to removed unincorporated nucleotides from labeling reactions.

·         Oligonucleotide Purification by PAGE and DEAE (Hahn Lab)

·         PAGE Purification of Oligonucleotides (Life Technologies)
Protocol for PAGE Purification of Oligonucleotides Protocol for PAGE Purification of Oligonucleotides Oligonucleotides contaminated by significant amounts of aborted synthesis products (e.g., n-1,...

Non-isotopic Labeling

·         PCR DNA Biotinylation (KPL)
A rapid method for biotinylating DNA probes through incorporation of biotin-N₄-dCTP via a thermostable DNA polymerase in the polymerase chain reaction

·         Determination of efficiency of digoxigenin labelling (Molecular Genetics Network Lab)

·         Non-isotopic Labeling (Gottschling Lab)
Protocols for preparing DNA, RNA probe and molecular marker

Others

·         PCR Products Labeling (Amersham)
Guidelines for preparation of radioactively-labeled PCR products for use as probes