SFX研究有哪些實例和參考文獻可供參考？

Figure 9 PS-I低分辨X－ray nanocrystallography 結構解析裝置示意圖及結構

答：第一次實驗。2011年，Henry Chapman在美國建成的LCLS自由電子激光裝置上成功獲得了光合反應中心I（PS-I）晶體的低分辨衍射數據。他們將尺寸在0.2-2微米的PSI晶體輸送到自由電子激光光束中，獲得了衍射數據。這些低分辨的衍射數據和在同步輻射上獲得的同樣晶體的數據完全一致，證明了利用XFEL可以獲得準確的晶體衍射數據。

Reference: Chapman, H.N., et al., Nature, 470 7332 (2011).

Figure 10 溶菌酶晶體的高分辨衍射數據

第一次室溫下高分辨。2012年Henry Chapman研究組利用LCLS的XFEL獲得了溶菌酶晶體的高分辨衍射數據，并利用分子置換法解析出了晶體結構。這次實驗使用的晶體尺寸很均一，為1X1X3μm3，獲得了1.8埃分辨率的溶菌酶晶體在常溫下的結構。此結果表明XFEL可以獲得高分辨的衍射數據，而且能夠解析出晶體結構。

Reference: Boutet, Sebastien., et al., Science, 337 6092 (2012).

Figure 11 一種GPCR的XFEL 結構解析

第一次GPCR等膜蛋白實驗。Henry Chapman報道了利用XFEL得到的GPCR的高分辨結構該實驗證明了膜蛋白結構也可以在XFEL上獲得，對于結構生物學研究者是很有吸引力的。

Reference: Liu, et al., Science, 342 6165(2013).

此外，還有大量的化學反應下的研究，動態構象變化的研究，泵浦下結構與性質的變化研究。代表性參考文獻：

1. H.N. Chapman, et al.,  "Femtosecond X-ray Protein Nanocrystallography" Nature 470, 73–77 (2011) .

2. Sébastien Boutet, et al., "High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography" Science 337 362 (2012) .

3. Lars Redecke, et al., "Natively Inhibited Trypanosoma brucei Cathepsin B Structure Determined by Using an X-ray Laser" Science 338 (2012).

4. Jan Kern,et al., "Simultaneous Femtosecond X-ray Spectroscopy and Diffraction of Photosystem II at Room Temperature" Science (2013).

5. D. Milathianaki, et al., "Femtosecond Visualization of Lattice Dynamics in Shock-Compressed Matter" Science 342 6155 (2013) .

6. Thomas R. M. Barends, et al., "De novo protein crystal structure determination from X-ray free-electron laser data" Nature -- (2013) doi: 10.1038/nature12773.

7. Wei Liu, et al.,  "Serial Femtosecond Crystallography of G Protein–Coupled Receptors" Science 342 1521 (2013).

8. Christopher Kupitz, et al., "Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser" Nature --- (2014) doi: 10.1038/nature13453.

9. Jason Tenboer, et al.,  "Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein" Science 346 1242 (2014) .

10. Yanyong Kang, et al.,  "Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser" Nature --- (2015) doi: 10.1038/nature14656.

11. Jose A. Rodriguez, et al., "Structure of the toxic core of α-synuclein from invisible crystals" Nature -- (2015) doi: 10.1038/nature15368.

12. Jose A. Rodriguez, et al., "Structure of the toxic core of α-synuclein from invisible crystals" Nature -- (2015) doi: 10.1038/nature15368.

13. Kanupriya Pande, et al., "Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein" Science 352 725(2016) .

14. J. R. Stagno, et al.,  "Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography" Nature --- (2016) doi: 10.1038/nature20599.

15. Iris D. Young, et al.,  "Structure of photosystem II and substrate binding at room temperature" Nature --- (2016) doi: 10.1038/nature20161.

16. A. Rudenko, et al., "Femtosecond response of polyatomic molecules to ultra-intense hard X-rays" Nature 546 129 (2017) doi: 10.1038/nature22373.

只列舉了我認為在不同方面具有突破性進展及影響力的Nature 及Science 文章。