CelldeathdetectioninXenopusembryosbyELISA
Sample preparationWash embryos 1 x in 25% MMRRemove excess bufferAdd 10 volumes "incubation buffer", i.e. 50 μl for 5 embryosLyse the embryos by gently pipetting up and down using a large blue tip Use a negative control, as shearing of genomic DNA may give false positive signalsDilute 10 μl lysate with 190 μl "incubation buffer" ......閱讀全文
Cell－death－detection－in－Xenopus－embryos－by－ELISA
Sample preparationWash embryos 1 x in 25% MMRRemove excess bufferAdd 10 volumes "incubation buffer", i.e. 50 μl for 5 embryosLyse the embryos by?gentl
In－Situ－Cell－Death－(Apoptosis)－Detection－by－TUNEL－labeling
Protocol for Frozen Sections:Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT.1x PBS rinse, 2 times.1x PBS, 30 min., RT. Beg
Effect－of－Cyclopamine－on－Xenopus－laevis－embryos
Cyclopamine and jervine, teratogens derived from the skunk cabbage?Veratrum californicum, are known to induce holoprosencephaly in chick embryos when
Whole－mount－TUNEL－analysis－of－Xenopus－embryos
Fixation and pretreatmentDejelly albino embryos carefully in 2% Cystein (pH 7.8).Remove the vitellin membrane with two pairs of tweezers???????? (or c
DEVELOPMENTAL－EFFECTS－OF－LITHIUM－CHLORIDE－ON－XENOPUS－EMBRYOS
PURPOSE: The objective of this experiment is to study the effect of lithium chloride treatment on early blastula?xenopus?embryos.INTRODUCTION: When th
發育生物學
In Vitro Production of Bovine Embryos?(P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)This protocol describe procedures for in vitro
SingleCell－Electroporation－in－Xenopus
Single-Cell Electroporation in XenopusXue Feng Liu and Kurt HaasINTRODUCTIONSingle-cell electroporation (SCE) is a versatile technique for delivering
Staining－Methods－for－cell－death
The simplest way: trypan blue.?Dead cells stain blueNon-fixed cells: FDA(fluorescein diacetate)-green, alive cells;?P.I. (propidium iodide)-red, dead
Guide－to－Cell－Proliferation－and－Apoptosis－Methods
Chapter 1: Cell Death - Apoptosis and Necrosis1.1Introduction21.1.1Terminology of cell death21.1.2Differences between necrosis and apoptosis31.1.3Apop
Protocol－to－Count－Cell－Number－of－Preimplantation－Embryos
Protocol to Count Cell Number of?Preimplantation?Embryos?using Nuclear Staining with Hoechst 33342 or DAPI??Introduction?The following is a simple pro
Detection－Of－Cell－Viability－And/Or－Apoptosis－By－Flow－Cytometry－(FACS)
Viable?cells are cells that when allowed to continue beyond the timepoint of examination will stay alive. Besides live and healthy cells, cells in ear
Dorsal－Lip－Transplant－in－Amphibian－Embryos
IntroductionIn early stages of development in amphibian embryos, very few cells' fates are rigidly determined. However, previous experiments show
Cell－Death－Differ：前列腺癌惡化標志
前列腺癌是近年來導致西方國家男性死亡的嚴重癌癥之一，擴散性疾病的患者治療情況也不夠樂觀。血清中的前列腺特意抗原（PSA）是用于癌癥檢測的分子標記，但是對于癌癥的惡化PSA并不能夠達到準確的預測，因此找到一個更加合適的分子標記對于前列腺癌的中期診斷十分重要。另一方面，由于前列腺癌的異質性，至今還沒
The－effects－ofdifferent－concentrations－oflithium－chloride－on－thedevelopment
AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular?Brachydanio rerio?embryos results in
Staining－Methods－for－cell－death－Z.－Xia－10/2/95
The simplest way: trypan blue.?Dead cells stain blueNon-fixed cells: FDA(fluorescein diacetate)-green, alive cells;?P.I. (propidium iodide)-red, dead
In－Cell－ELISA
實驗概要This protocol is a general protocol for ICE analysis and can be used with any of Abcam's ICE-validated antibodies. Antibodies are availabl
A－study－of－Fetal－Alcohol－Syndrome
IntroductionFetal Alcohol Syndrome (FAS) is caused by exposure of the developing embryo to alcohol, one of several teratogenic agents which adversely
Effect－of－ethanol－on－development－of－Danio－reriro
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
同濟大學毛志勇教授發表Cell－Death－－Differentiation文章
基因組穩定性下降是生物體衰老發生極其重要的一個標志。細胞長期在各種因素的影響下，DNA遭受著多種損傷，若這些損傷不被及時準確地修復將誘發基因組穩定性的下降，進而影響細胞的正常生命活動。這些損傷中，DNA雙鏈斷裂（DSBs）是最為嚴重的基因組損傷之一。近年來，雖然關于DNA DSBs修復與衰老發
Cell－Death果蠅DNA損傷后細胞增殖與凋亡的協調
暴露于遺傳毒性應激可促進細胞周期停滯和DNA修復或凋亡。這些“生”或“死”細胞命運的決定通常取決于腫瘤抑制基因53的活性。因此，對p53的精確調控對于維持組織內環境平衡和防止腫瘤的發展至關重要。然而，細胞周期進程是如何影響p53細胞命運的，目前尚不清楚。 已經提出了幾種機制來解釋dna損傷細胞
Cell－Death－Dis：中風患者遭受腦損傷的新機制
近日，研究人員發現中風患者遭受腦損傷的機制，并正在尋找藥物來阻止它。 當供應到大腦的血液被部分切斷時中風發生，但對于存活者而言更嚴重的傷害是記憶和其他認知功能傷害，這些記憶和其他認知功能傷害常常實際上由血液供應恢復后的幾小時或幾天內“氧化應激”生成過多所引起的。 從Leeds大學和中國浙江大
Investigating－the－effect－ofthe－teratogen－cyclopamine－on－Ambystoma－mexicanum
ObjectiveThis experiment attempted to determine the effects of the teratogen cyclopamine on the Shh signaling pathway in axolotl embryos. Groups of ax
Coenzyme－A－Detection
實驗概要The experiment provided ?an ?easy, convenient assay to measure the CoA level in a variety of ?biological samples. In the assay, free CoA is specif
Cell－Death－Differ：啟動肺癌細胞自毀開關的新療法
近日，英國癌癥研究中心科學家們發現了一種藥物組合，可以觸發肺癌細胞的自毀過程，此項研究為新型治療方法開發鋪平了道路。 當健康細胞對機體不再有用，它們便引發一連串事件最終導致自我毀滅。但癌細胞則突然轉向遠離這個“自殺道路”，成為不朽，這意味著細胞生長失控，引起腫瘤的形成。 現在，英國癌癥研究中
第一屆Cell－Death－and－Disease研討會會議通知
第一屆Cell Death and Disease研討會暨第四屆中英“細胞死亡、干細胞與癌癥”國際研討會將于2013年5月8日~9日在上海召開。 本次會議主題為：細胞死亡、干細胞與免疫，將邀請海內外相關領域卓有建樹的科學家，采用大會報告及專題討論的形式，結合國際上癌癥生物學研究領域的前
Cell－Death－－Disease免疫、干細胞與疾病－國際研討會召開
10月12日至14日，第五屆Cell Death & Disease免疫、干細胞與疾病國際研討會在中國常州市明都真儒酒店會議廳召開。常州市副市長張云云、衛生局黨委書記、局長王莉、常州市第一人民醫院院長何小舟、中國科學院上海生命科學研究院健康科學研究所主持工作的副所長孔祥銀等領導出席會議開幕式并講
Fixation－of－Embryos
MEMFA Fix10xMEMFA Salts1 part 10x MEMFA salts1 M MOPS1 part 37% formaldehyde20mM EGTA8 parts water10mM MgSO410x salts can be autoclaved and stored. Tu
細胞遺傳學——原位雜交(ISH)
In Situ Hybridization· ????????In Situ Hybridization?(jsmith1@po-box.mcgill.ca)In situ?hybridization, as the name suggests, is a method of localizing,
cell－death－－disease:－黑色素瘤抗壞死效應分子機制
RIPK1與RIPK3被發現是參與細胞壞死性凋亡的關鍵成員，在caspase家族活性被抑制的情況下，RIPK1激活后能夠與下游的RIPK3結合形成壞死小體，幫助RIPK3激活（磷酸化）。磷酸化后的RIPK3再次將MLKL磷酸化，磷酸化后的MLKL能夠在細胞膜內側自聚合形成寡聚體，最終導致細胞壞死
Cell－Death－and－Disease:－RNF128有望成為ALI藥物干預的候選藥物
急性肺損傷(ALI)和急性呼吸窘迫綜合征(ARDS)是以嚴重的肺部炎癥、肺泡毛細血管屏障受損和肺水腫為特征的危及生命的呼吸系統疾病，死亡率高。ALI發生發展的分子機制尚未完全闡明，但肺泡巨噬細胞和中性粒細胞在ALI的發病機制中起著關鍵作用。 激活的中性粒細胞和肺泡巨噬細胞釋放過量的促炎細胞因子