SeparationofPlateletsfromWholeBlood
PurposeThis protocol describes how to isolate human platelets from whole blood. Isolated platelets are used for static adhesion assays, for flow chamber assays, flow cytometry measurements, etc.MaterialsCitrate buffer (25 g sodim citrate, 8 g citric acid, 500 ml H2O2).Prostaglandin E1 (PGE1).ACD buffer (6.25 g sodium citrate.2 H2O , 3.1 g citric acid anhidrous, 3.4 g D-glucose in 250 ml H2O).Hepes-Tyrode buffer ......閱讀全文
Separation－of－Platelets－from－Whole－Blood
PurposeThis protocol describes how to isolate human platelets from whole blood. Isolated platelets are used for static adhesion assays, for flow chamb
Serum－Separation－from－Whole－Blood
Serum Separation from Whole Blood1) Collect sample (preferably in glass tubes) and leave for 1 hour at 37°C to allow it to clot.2) Leave sample at 4°C
Method:－Lymphoblastoid－Cell－Lines－from－Frozen－Whole－Blood
Method: Lymphoblastoid Cell Lines from Frozen Whole BloodMay 31, 1990Rosalie VeilePurpose:Blood Samples can be stored frozen as a backup in case an LC
DNA－Extraction－from－Blood
實驗概要The ChargeSwitch? ?gDNA Purification Kits allow rapid and efficient purification of ?genomic DNA from small volumes of human blood. After preparin
Protein－extraction－from－whole－tissues－for－IEF
Modified from that of Jay Thelen - University of Missouri-ColumbiaPhenol extraction followed by methanolic ammonium acetate precipitation - an effecti
Examination－of－a－Mammalian－Blood－Smear
Examination of a Mammalian Blood SmearThe distribution and appearance of the formed elements of blood can tell a great deal about the condition of the
Harvesting－lymphocytes－from－Peripheral－Blood
Blood Harvest·??????? Anesthetize mice with 50ml of Xylene-Ketamine(2:1) coctail·??????? Pin animal to a board (use distal extremities) supine·???????
Standard－Protocol－For－Up－to－10－ml－Whole－Blood
實驗概要The E.Z.N.A.? ?Blood DNA Maxi Kit is designed for isolation of genomic DNA from up to ?25 ml of fresh, whole blood treated with any common anticoa
SQ－Blood－DNA－Maxi－Protocol－for－410－ml－whole－blood
實驗概要The E.Z.N.A.? ?SQ Blood DNA Kit is designed for isolating high molecular weight ?genomic DNA from fresh, frozen or anticoagulated whole blood. The
Protocol－for－Isolating－DNA－from－Blood－with－Nucleated－Red－blood－Cells
實驗概要DNA ?isolation from fish or avian blood sample can be difficult because it ?contains nucleated red blood cells. E.Z.N.A. NRBC Blood DNA Kit is ?de
SQ－Blood－DNA－Midi－Protocol－for－500l3ml－whole－blood
實驗概要The E.Z.N.A.? ?SQ Blood DNA Kit is designed for isolating high molecular weight ?genomic DNA from fresh, frozen or anticoagulated whole blood. The
Sample－preparation－(analytical－gels)
Sample preparation and solubilization are crucial factors for the overall performance of the 2-D PAGE technique. Protein complexes and aggregates shou
DNA－Purification－from－Blood－or－Body－Fluids
實驗概要試劑盒，操作簡單，稍有點貴，還可以接受。實驗材料blood實驗步驟Protocol: DNA Purification from Blood or Body Fluids(Spin Protocol)This protocol is for purification of total (ge
Direct－PCR－from－Whole－Yeast－Cells:－Zymolyase－Method
Direct PCR from Whole Yeast Cells: Zymolyase MethodContributor: Namjin ChungDate: June 18, 19961. An average-size yeast colony (0.5-2mm) or a cell pel
Plasma－and－Serum－Preparation
實驗概要Serum is the ?liquid fraction of whole blood that is collected after the blood is ?allowed to clot. The clot is removed by centrifugation and the
Preparation－of－human－platelets
Preparation of human platelets????? 1.?Human blood was taken from drug-free volunteers on the day of the experiment using acidic citrate dextrose
Isolate－DNA－from－NRBC－Blood－from－Buccal－Swab－collected－with－filter－paper
實驗概要DNA ?isolation from fish or avian blood sample can be difficult because it ?contains nucleated red blood cells. E.Z.N.A. NRBC Blood DNA Kit is ?de
巨噬細胞和單核白細胞
·?????????Lymphocyte Transformation?(Donis-Keller lab)Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocytes) from antico
MaxiYield－protocol－for－10－20－ml－Whole－Blood
實驗概要The E.Z.N.A.? ?Blood DNA Maxi Kit is designed for isolation of genomic DNA from up to ?25 ml of fresh, whole blood treated with any common anticoa
Isolation－of－PBMCs－from－patient－blood－and－buffy－coats1
1| Transfer heparinized venous blood of patient to plastic50-ml tubes and dilute with an equal volume of PH buffer.When sodium citrate has been used a
Vacuum/Spin－Protocol－for－Isolating－DNA－from－Blood－with－Nucleated－Red
實驗概要DNA ?isolation from fish or avian blood sample can be difficult because it ?contains nucleated red blood cells. E.Z.N.A. NRBC Blood DNA Kit is ?de
CMFDA－Labeling－of－Platelet
OUTLINECMFDA (5-chloromethylfluorescein diacetate) is a lipophilic tracer that has an enormous advantage over ordinary tracers (e.g. FITC) because it
Human－Peripheral－Blood－Mononuclear－Cell－Preparation
This protocol describes a procedure for isolating human peripheral blood mononuclear cells (lymphocytes and monocytes) from a Buffy Coat (obtained fro
細胞培養常規操作
常規操作(主要內容如下)·?????????Aseptic Technique·?????????Culture Vessels·?????????Cell Counting·?????????Primary Culture·?????????Maintenance of Cell Line?·??
Intrinsic－Prothrombin－Activation－Pathway
Blood coagulation or clotting takes place in 3 essential phases. The first phase is the activation of a prothrombin activator complex. The second phas
關于冰凍血小板的質量
"冰凍前及融化后使用前22±2℃平衡4h"肯定是沒有必要的。"37℃靜止水浴融化"要改為38℃循環水浴融化。將冷卻速度控制在－1℃/min（不是2±1℃/min）左右尤其是-15℃～-40℃之間是關鍵；運輸不能用冰盒，否則冰凍保存失敗（常見）；冷凍時冰箱溫度低于-60℃（放入時開蓋時間過長或冰箱制冷
Bleeding－Time－Test
OUTLINEAlthough in the Review Article : "Idiopathic Thrombocytopenic Purpura: A practice Guideline Developed by Explicit Methods for the American Soci
Separation－of－sea－urchin－blastomeres
Objective: to separate the Blastomere at different points in development and observe the growth of the resulting embryos.Procedure:?We extractrf the g
Coating－of－Platelets－with－Antibody－in－vitro
OUTLINEAntibody-coated platelets (opsnized) may be used in the subsequent thrombophagocytosis assay.?PROTOCOLResuspend 1.6x10^8 of CMFDA-labeled plate
Method:－Lymphocyte－Transformation
Method: Lymphocyte TransformationMay 30, 1990Rosalie VeilePrinciple:Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocyte