TandBcellIsolation
Reagents Heparin - 1000 U/ml Ficoll-Hypaque PBS RPMI-1640 supplemented with 10 mM glutamine and 15% FBS AET (0.14M) Dissolve 1.967 g AET in 35 ml di-H2O. Adjust to pH 8.0 with 1.0N NaOH. Bring volume to 50 ml with di-H2O.Store at 2-8oC. Check pH every 2 weeks. AET-treated SRBC Wash SRBC 4 times with PBS Add 4 volumes AET to 1 volume packed SRBC in a 15 m conical tube......閱讀全文
T－and－B－cell－Isolation
Reagents?Heparin - 1000 U/ml?Ficoll-Hypaque?PBS?RPMI-1640 supplemented with 10 mM glutamine and 15% FBS?AET (0.14M) Dissolve 1.967 g AET in 35 ml di-H
細胞分離技術（Cell－Isolation）
一、離心技術離心是研究如細胞核、線粒體、高爾基體、溶酶體和微體，以及各種大分子基本手段。一般認為，轉速為10~25Kr/min的離心機稱為高速離心機；轉速超過25Kr/min，離心力大于89Kｇ者稱為超速離心機。目前超速離心機的最高轉速可達100Kr/min，離心力超過500Kg。（一）、差速離心（
Human－thyroid－endothelial－cell－isolation
1.?Human thyroid glands were obtained at surgery from multinodular goiters.2.?The tissue was incubated in 0.1% type II collagenase (Worthington) overn
Human－B－cell－isolation－and－culture
B cell isolation1.?Donor blood was obtained with informed consent under guidelines issued by the Medical Ethics Committee. 2.?Mononuclear cell fractio
Follicular－thyroid－cell－isolation－and－culture
Thyroid epithelial cells (also called follicular cells or principal cells) are cells of thyroid gland that are responsible for the production and
Human－B－cell－isolation－and－culture
實驗概要This protocol provides a general protocol for human B cell isolation and culture.實驗步驟B cell isolation1.?Donor blood was obtained with informed con
Primary－brain－cell－isolation－and－culture
1.?Cerebella were removed from 7-day-old mice and passed through Nitex nylon netting (80 μm pore size) into primary cell system containing 20% (v/
Isolation－of－dermal－mast－cell－(DMC)
1.?Human DMC were isolated from skin by enzymatic digestion.2.?Dermal tissue was obtained from patients with skin after informed consent was given.3.?
Isolation－and－culture－of－smooth－muscle－cell
1.Male Wistar rats of about 250g body wt were anesthetized with ether and were decapitated. ?2.Kidneys and thoracic aorta were removed, rinsed with
Isolation－of－cell－nuclei－for－the－application－in－the－cellfree－system
Characteristics of the procedurePreparation of isolated nuclei - procedurePreparation of radioactive labeled nucleiMaterial?Characteristics of the pro
Isolation－of－retinal－pigment－epithelial－cell（二）
15.?This medium is replaced after 1 day with 5% serum-containing RPE medium, and subsequent changes were made every 2 to 3 days.16.?After 3 to 4 weeks
Isolation－of－retinal－pigment－epithelial－cell（一）
The pigmented layer of retina or retinal pigment epithelium (RPE) is the pigmented cell layer just outside the neurosensory retina that nourishes
Mouse－B－cell－isolation－with－magnetic－beads
實驗概要Isolate B cells from total mouse cells實驗步驟?Praparation:1.?????? Enough MACS; put RPMI into water bath.2.?????? Put some MACS in 15 ml tube or 1.5
RNA－Isolation－From－Animal－tissue－or－cell－culture
實驗概要This method is ?designed for most animal tissues and culture cells. For RNA isolation ?from fibrous tissue, follow the specialized protocol on pag
Isolation－and－Culture－of－Human－Brain－Tumor－Stem－Cell
The isolation, culture, identification, and purification of stem cells from primary human brain tumors of different phenotypes have marked capacit
Isolation－of－Cell－Wall－Proteins－from－Medicago－sativa－Stems
Plant cell walls are highly dynamic and chemically active components of plant cells. Cell walls consist primarily of polysaccharides, with protein
Mitochondrial－DNA－Isolation－from－Somatic－Embryogenic－Cell－Cultures－of－Larix
Mitochondrial DNA is isolated by a modification of the methods described by Wilson and Chourey (1984) and Radetzky (1990).?Cell cultures at four days
Isolation－of－mouse－embryos
1. Sacrifice impregnated mouse.2. Dissect out the uterus of the mouse. Pulling up on the uterus with one set of forceps,use another to tear the mesome
mice－islet－isolation
1.?Islets of Langerhans were isolated from 5- to 7-week-old nonobese diabetic (NOD) mice. 2.?Which involves cannulation of the common bile duct and
Yeast－Nuclei－Isolation
This method gives yeast nuclei which look nearly purified microscopically. Nuclei isolated in this way do not give active transcription extracts when
Isolation－of－murine－splenocytes
OverviewIn order to study spleen cells (e.g. lymphocytes, granulocytes, other immune cells), it helps to make single-cell suspensions so that the cell
Isolation－of－liver－lymphocyte
Isolation of liver lymphocyte???Several lymphocyte subpopulations reside in the normal adult human liver. Liver lymphocytes mainly include a large n
Isolation－of－papillary－cells
Isolation of renal papillary cells1.?For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/
Isolation－of－Zebrafish－embryos
Zebrafish will mate and deposit fertilized eggs on the bottom of the tank at 'dawn'. They can be accustomed to lay at any convenient time by k
Fungal－DNA－Isolation
Fungal DNA IsolationSaghai-Maroof MA, Soliman KM, Jorgensen RA, & Allard RW (1984) PNAS 81:8014-8018DNA successfully isolated from fungal species of C
Arabidopsis－gDNA－isolation
This is a simple and fast protocol for the extraction of genomic DNA from Arabidopsis thaliana that works fine in PCR for simple amplicons. We only us
Cosmid－DNA－Isolation
實驗概要Isolation of high yields of highly pure cosmid DNA using PureLink? HiPure Plasmid Purification Kits.實驗原理The ?PureLink? HiPure Plasmid Purification
Isolation－of－colonic－epithelium
The method we use is based on work of Dr. Hazel Cheng, at the University of Toronto and works for both colon and the small intestine.First we excise t
Chromosomal－DNA－Isolation
Chromosomal DNA IsolationMETHOD:Grow cells in 2-5 ml broth to late log phase.Pellet 1-2 ml cells in microfuge.Resuspend cells in 400 μl TES (50 mM Tri
Isolation－Of－PCR－Products
實驗概要Rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-nucleic acid reagents.實驗原理The ChargeSwitch? TechnologyT