45Ca2+uptakestudyinPC12cells
45Ca2+ uptake study in PC12 cells 1. Plate the cells on to the poly-D-Lysine-coated six well plates 2 days prior to study. 2. Rinse the cells with 1 ml secretion buffer (150 mM NaCl, 5mM KCl, 2mM CaCl2, 10 mM Hepes, pH 7.4) every 15 minutes for 1 hour at 37oC. 3. Add 45Ca2+ (2 μCi/ml) to Ca2+ -free buffer (secretion buffer without 2 mM CaCl2), and drugs to the labelled buffer at 37oC. 4. Incubate ......閱讀全文
45Ca2+－uptake－study－in－PC12－cells
45Ca2+ uptake study in PC12 cells?1. Plate the cells on to the poly-D-Lysine-coated six well plates 2 days prior to study.?2. Rinse the cells with 1 m
HThymidine－Uptake－by－Cultured－Cells
H-Thymidine Uptake by Cultured CellsLEVEL IIMaterialsFibroblast cells in log phase growthCa, Mg?free-phosphate buffered saline (PBSA)5% (w/v) Glutaral
3HThymidine－Uptake－by－Cultured－Cells
Materials Fibroblast cells in log phase growthCa , Mg free-phosphate buffered saline (PBSA)5% (w/v) Glutaraldehyde (GTA)2% (w/v) Perchloric Acid (PCA)
3HThymidine－Uptake－by－Cultured－Cells
3H-Thymidine Uptake by Cultured Cells ?OverviewA radioactive method for measuring cultured cell growth with 3H-thymidine is described here.?MaterialFi
mpulsive－Pressurization－of－Neuronal－Cells－for－Traumatic－Brain－Injury－Study
實驗概要A novel impulsive ?cell pressurization experiment has been developed using a Kolsky bar ?device to investigate blast-induced traumatic brain injur
體外熒光法檢測核內體早期動力學4
Top?of pageProcedureOverviewSteps 1 - 8 Preparation of rat brain cytosolSteps 9 - 10 Culture of PC12 cellsSteps 11 - 30 Preparation of postnuclear sup
體外熒光法檢測核內體早期動力學
A fluorescence-based?in vitro?assay for investigating early endosome dynamicsSina V Barysch1,2, Reinhard Jahn1?& Silvio O Rizzoli2ABSTRACTEarly endoso
New－Case－Study－Released
The Dutch grocery retailer Spar decided to manage all its retail weighing scales centrally from the company’s headquarters using the METTLER TOLED
A－study－of－Fetal－Alcohol－Syndrome
IntroductionFetal Alcohol Syndrome (FAS) is caused by exposure of the developing embryo to alcohol, one of several teratogenic agents which adversely
PC12細胞培養
PC12細胞是大鼠腎上腺髓質嗜鉻瘤分化細胞株，具神經內分泌細胞的一般特征，因其具可傳代特點，廣泛應用于神經生理和神經藥理學研究。1. PC12細胞有兩種：未分化型和分化型。其中未分化型的PC12細胞貼壁能力強，傳代時需要胰酶消化，形狀不規則。分化型PC12細胞傳代時不需要胰酶，直接可以吹下來，形狀規
PC12－Cell－Culture－and－Fusion
Cell CultureMaterials1. Falcon Primaria culture dishes.2. Culture medium: DME (or F12K) with glutamine, supplemented with 7% heat-deactivated horse se
Green－Fluorescent－Protein－as－an－Indicator－ofTransfection－in－Chicken－Embryos
Green fluorescent protein (GFP) is responsible for the bioluminescence of the Pacific Northwest jellyfish, Aequorea victoria. In A.victoria, the 27-kD
PC12細胞培養方法步驟
PC12細胞是大鼠腎上腺髓質嗜鉻瘤分化細胞株，具神經內分泌細胞的一般特征，因其具可傳代特點，廣泛應用于神經生理和神經藥理學研究。1. PC12細胞有兩種：未分化型和分化型。其中未分化型的PC12細胞貼壁能力強，傳代時需要胰酶消化，形狀不規則。分化型PC12細胞傳代時不需要胰酶，直接可以吹下來，形
線粒體腫脹檢測方法
Estimating Mitochondrial Mass，可以用于線粒體腫脹的檢測Accurate measurements of mitochondrial mass require a probe that will accumulate in mitochondria regardless
Culture－of－BEND－Cells－(Bovine－Endometrial－Cells)
Culture of BEND Cells (Bovine Endometrial Cells)Charles E. Krininger, III and Peter J. Hansen?Dept. of Animal Sciences, University of FloridaThis prot
Differentiate－ES－cells－into－glial－cells－and－neurons
Day -1: Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells.___________________Day 1:?Trypsiniz
軟件：Genomewide－Association－Study－Software
The software included are listed bellow?IMPUTEa program for genotype imputation in genome-wide association studies and fine-mapping?studies based on a
Optimized－Fibrin－Gel－Bead－Assay－for－the－Study－of－Angiogenesis
PREPARING CELLSBring up HUVEC and fibroblasts in M199/10% FBS/Pen-Strep (1:100) 1-2 days before beading.Switch medium to EGM-2 (Clonetics) the day bef
線粒體熒光探針大全：TMRM，Mitotracker，JC1（3）
Mitochondrion-Selective Rhodamines and RosaminesRhodamine 123Rhodamine 123 (R302; FluoroPure Grade, R22420; Figure 12.22) is a cell-permeant, cationic
Differentiating－Neural－Stem－Cells－into－Neurons－and－Glial－Cells
實驗概要The protocols in ?this section describe the steps involved in differentiating neural stem ?cells (NSC) to neurons, astrocytes, and oligodendrocyte
Use－of－Nonaqueous－Fractionation－and－Metabolomics－to－Study－Chloroplast－...
Use of Non-aqueous Fractionation and Metabolomics to Study Chloroplast Function in ArabidopsisChloroplasts are the chemical factories of plant cells b
Growing－cells
No two cell lines behave exactly the same, so you must learn the peculiarities, or personality, of each of the cell lines with which you work. Irrespe
Trypsinizing－cells
There are many procedures with which to trypsinize cells. All include washing the cell monolayer with TD, or in rare cases, with VE. This removes seru
Lyophilizing－Cells
Lyophilizing CellsInoculate 200 ml L-broth supplemented with appropriate antibiotics with the bacteria to be lyophilized.Incubate the culture at 37°C
Lyophilizing－Cells
Inoculate 200 ml L-broth supplemented with appropriate antibiotics with the bacteria to be lyophilized.Incubate the culture at 37°C with vigorous shak
Freezing－Cells
1) Keep prepared solutions on ice.2) Determine total cell count of cells to be frozen. (e.g. 1 X 108 )3) Determine number of vials to be frozen. (e.g.
BTG－family－proteins－and－cell－cycle－regulation
BTG2 is found to be one of the immediate early genes up-regulated by neural growth factor (NGF) and epidermal growth factor (EGF). Its transcriptional
柴之芳：Hybrid－MS－in－metallomics－and－metalloproteomics：A－case－study
中國科學院高能物理研究所 柴之芳院士 2014年4月26日，首屆全國質譜分析學術研討會在北京西郊賓館盛大開幕。來自中國科學院高能物理研究所的柴之芳院士帶來了題為《Hybrid MS in metallomics and metallo
體外熒光法檢測核內體早期動力學5
Leave tubes on ice and repeat Steps 13–15 for the next 6–12 plates of cells.When all cells are collected, centrifuge all tubes at 250g?for 5 min at 4
PC12細胞培養所用血清問題
問：我正準備購買上海細胞所的未分化的PC12細胞，需要滅活的馬血清，可現在買血清很困難，好像是海關有封鎖，代理商這么說的，我原定購買的Gibco的horse surum，現在無法買到了，好容易找到一個目前可以進血清的公司Hyclone，有一種Donor Equine serum是馬血清嗎？答：H