HowdoyousynthesizeyourdsRNA
We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends (I. Primer Designed dsRNA). It is also possible to produce dsRNA using PCR generated DNA templates containing either the T7 & SP6 or the T7 & T3 promoters on either end (II. dsRNA Fom Clones). This method is less efficient, especially when working on a large scale.All work shou......閱讀全文
How－do－you－synthesize－your－dsRNA
We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends (I. Primer Desig
How－do－I－decontaminate－my－tissue－culture－(Invitrogen)
When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination. First, determine if the contami
Creation－and－Use－of－Infectious－Virus－Vector
Creation and use of your infectious vector:Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 ml of media. (This can be scaled up if desired).The f
Degenerate－PCR,－a－short－guide.
What is degenerate PCR????Degenerate PCR is in most respect identical to ordinary PCR, but with one major difference. Instead of using specific PCR pr
CREATION－AND－USE－OF－YOUR－INFECTIOUS－VECTOR
實驗概要CREATION AND USE OF YOUR INFECTIOUS VECTOR實驗步驟Day 1? ? ? ? 1. Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 mL of media. (This can be scal
Protein－Expression－and－Purification－Protocol
Step 1:?Transform?appropriate DNA plasmid into BL21(DE3)?E. coli?cells. These cells must be competent. (Protocol for how to make competent cells.)a) T
Gel－Electrophoresis－of－DNA
What is Electrophoresis?Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. In this CyberLab we
Belcher/Knight:－Electrocompetent－Cells
Electrocompetent CellsFrom Danijela Dukovski at Harvard Medical School. This protocol works well. --Julie NorvilleMaterialsDI water10% GlycerolSpecial
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Log in radioactive material received and deduct amount of radioactive material used in each experiment in Radioisotope Log Book.Clear your bench top w
Degenerate－PCR
Degenerate PCR is in most respects identical to ordinary PCR, but with one major difference. Instead of using specific PCR primers with a given sequen
SYBR－Green－Quantitative－PCR－Protocol
SummaryQuantitative PCR is a method used to detect relative or absolute gene expression level. All qPCR involves the use of fluorescence to detect the
Basic－Method－for－Indirect－Immunofluorescence－Labeling
Basic Method for?Indirect?Immunofluorescence LabelingBackgroundThis is the method for?indirect?immunofluorescence labeling; that is, the antibodies?do
sothing－about－Genome－walking
Can anyone recommend a good kit for genome walking? I would like to find out the promoter sequence of a known gene. Is genome walking the way to do it
軟件技術如何幫助開發更精確有效的體外診斷系統？
The use of?in vitro?tests often plays an important role in the diagnosis and treatment of disease. However, many of these tests rely on detecting
4－Reasons－Why－Your－Tank－Scale－Could－Be－Wrong
Do you rely on tank scales when filling, batching or tracking inventory? If so, accuracy limitations can diminish product quality, leading to mate
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1) Turn on the counter by pulling out the on/off button. You need to do this at least 10 min before use to obtain sufficient vacuum.Usually put 0.2 ml
Phosphoamino－acid－analysis:Mark－Kampss－method
Phosphoamino acid analysis:Mark Kamps's method1.?Label your protein with 32Pi.?Then subject the protein to SDS polyacrylamide gel electrophoresis
PCR－Primers－For－Gene－Expression－Detection－or－Quantification
Why PrimerBank?PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time
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Good Pipetting PracticePipetting Techniques to Boost Your PerformanceImprove your data quality with Good Pipetting Practice? (GPP?) – METTLER TOLE
annealing－temp－for－degenerated－primer－－PCR－problem
PCR primers should be free of significant complementarily at their 3'-termini as this favours formation of primer-dimer which reduce product yield
Protocol－for－dsRNA－Synthesis
實驗概要? ? ? ? We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends
果蠅RNAi的實驗中雙鏈短RNA的合成（dsRNA)方法
實驗概要We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends (I. Prim
針對特定細胞選擇更有效的實驗步驟
GenJet Ver.II，LipoD293及PolyJet針對哺乳動物細胞的DNA體外轉染試劑，均為您提供兩種不同的轉染步驟——一般步驟及高級步驟，這兩種步驟分別針對不同的哺乳動物細胞。高級步驟主要針對難轉的哺乳動物細胞，例如：MDCK，MDA-MB231，Caco-2等細胞。使用一般的轉染步
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eBioscience公司Luminex液相蛋白芯片檢測試劑——ProcartaPlex可以為您的生物學研究提供各種多因子檢測方案，包括細胞因子、生長因子、趨化因子和其他蛋白。在選擇試劑盒及操作時會遇到很多問題，現將常見問題及答復匯總如下，供您參考： 如果不小心將整個試劑盒保存在-20°C. 這個試
FIXATION－and－DNA－Staining－for－Cell－Cycle－Analysis
BackgroundThis method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to ente
果蠅RNAi的實驗中雙鏈短RNA的合成（dsRNA)方法
本文來自于哈佛大學醫學院果蠅RNAi篩選中心的經典實驗方法，專門用于果蠅RNAi實驗方法。感謝哈佛大學醫學院果蠅RNAi篩選中心的支持！Primer Designed dsRNATemplate SelectionPCRIn vitro?RNA TranscriptiondsRNA Purifica
How－to－interrupt－scintillation
Peter Novick Lab, Department of Cell Biology Yale University School of Medicine HOW TO PERFORM AN INTERRUPT OF ANONGOING AUTOCOUNT1). This program int
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Tissue－Culture－Methods3
REFERENCES:R. Ian Freshney,?Culture of Animal cells: A manual of basic techniques,?Wiley-Liss, 1987.VI. TISSUE CULTURE PROCEDURESEach?student?should m
Dropout－plates－for－yeast
Materials(Solutions are all available from the media room)200ml bottle of 2x SD200ml bottle of 4% agar -- make sure to sign it out40% glucoseCSM minus