BasicMechanismsofSUMOylation
Like ubiquitin, SUMO (small ubiquitin-related modifier) proteins are small protein tags that are conjugated to proteins to modify their function. The ubiquitin system tags proteins for degradation by the proteosome (see Proteosome pathway) but SUMO conjugation has a range of other functions, stabilizing some proteins and altering their subcellular localization. Sumoylation may also influence ubiquitination and protei......閱讀全文
Basic－Mechanisms－of－SUMOylation
Like ubiquitin, SUMO (small ubiquitin-related modifier) proteins are small protein tags that are conjugated to proteins to modify their function. The
Mechanisms－of－transcriptional－repression－by－DNA－methylation
Tumorigenesis is known to be a multistep process in which defects in various cancer genes accumulate. Epigenetic modifications, most importantly DNA m
Basic－PCR
實驗概要The ?following basic protocol serves as a general guideline and a starting ?point for any PCR amplification. Optimal reaction conditions (incubati
Basic－ELISA－Protocol
實驗概要? ? ? ? There are many different types of ELISAs, which can detect the presence of protein in serum or supernatent. One of the most common typ
Nature：SUMOylation水平與細菌感染
?在感染過程中，食源性病原體“單核細胞增生李斯特菌”能利用宿主細胞的大量功能，包括涉及泛素化和磷酸化的、專門修飾關鍵蛋白活性的轉錄后修飾。致病細菌對被稱為“SUMOylation”的泛素樣修飾（真核細胞中一個重要過程）()的效應在很大程度上仍不清楚。現在，對被“單核細胞增生李斯特菌”感染的人類細胞和
Basic－Protein－Chemistry－Techniques
實驗概要Basic Protein Chemistry Techniques實驗步驟Coomassie Blue Stain:? (for gels)?1) Combine 225 ml Methanol with 225 ml ddH2O.?2) Add 0.5 grams of Coomassi
Basic－Protein－Chemistry－Techniques
Coomassie Blue Stain:? (for gels)?1) Combine 225 ml Methanol with 225 ml ddH2O.?2) Add 0.5 grams of Coomassie Blue.?3) Just before use, add 50 ml acet
Basic－Methods－of－Culturing－Drosophila
實驗概要Basic Methods of Culturing Drosophila實驗步驟Stockkeeping1. Mechanics? ? ? ? Most stocks can be successfully cultured by periodic mass transfer of a
Sumoylation－by－RanBP2－Regulates－Transcriptional－Repression
Current data indicates that nuclear pore complex (NPC) RanBP2 (SUMO E3 ligase) protein sumoylates Mdm2 and HDAC proteins during nuclear translocation.
篩分儀－AS－200－basic簡介
德國RETSCH（萊馳）的AS200系列分析篩分儀廣泛應用于科研與開發，原材料、半成品和成品的質量控制及生產監控等領域。其可控電磁驅動能為每一產品提供最優的配置。尖銳樣品也能在短時間內過篩。AS200basic具有RETSCH 產品一貫的可靠性和質量保證。該機型采用模擬制式下的振幅和篩分時間設置。所
Basic－Method－for－Indirect－Immunofluorescence－Labeling
Basic Method for?Indirect?Immunofluorescence LabelingBackgroundThis is the method for?indirect?immunofluorescence labeling; that is, the antibodies?do
Basic－Fluorescent－in－situ－Hybridization－(FISH)
實驗概要Fluorescence ?in situ hybridization method is a kind of physical map drawing method, ?use fluorescent element mark probe, to detect probe and spli
SUMOylation－as－a－mechanism－to－modulate－CtBPdependent－gene－responses
Covalent attachment of one eukaryotic protein to another is a prominent posttranslational modification and Ubiquitin is the most familiar of the prote
Basic－procedures－for－bacteria－culture1
A. Phenol extraction of DNA samplesPhenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated
Basic－procedures－for－bacteria－culture2
E. Elution of DNA fragments from agaroseDNA fragments are eluted from low-melting temperature agarose gels using an unpublished procedure first develo
研究揭示雙微體形成分子機制(Molecular－Mechanisms)
在線發表于2013年2月5日《International Journal of Cancer》雜志上的一篇新論文De novo-generated small palindromes are characteristic of amplicon boundary junction of do
Basic－Theory－and－Use－of－GCMS（二）
For?ionisation?to?take?place?at?all,?chemical?reaction?between?the?sample?and?the?reagent?gas?must?be?exothermic.?The?grater?the?heat?of?the?reaction,
Basic－Theory－and－Use－of－GCMS（一）
BASIC?THEORY?AND?USE?OF?GC-MSbyDr.?Eugenia?SobolevaContent1.??Introduction.2.??GC-MS?systems?and?components.3.??Vacuum?system3.1.??Rotary?pump3.2.??Di
AvaSoftBasic標準版軟件
AvaSoft-Basic標準版軟件AvaSoft-Basic標準版軟件是是隨每套AvaSpec光譜儀系統免費贈送的標準軟件包。AvaSoft-Basic軟件界面友好，由鼠標指向控制下拉菜單。點擊鼠標可以控制數據指針的移動，可以即時讀取波長﹑像素值和?Y軸幅值。而按住鼠標左鍵或右鍵并拖曳可以方便并精
篩分儀Sieve－Shaker－AS－450－basic簡介
AS450 basic是強力振蕩篩分儀AS450 control的基礎版。 它用于干篩，篩分范圍從 25 μm至125 mm，最大負載達15 kg。時間和振幅都是數字可調，可儲存1組參數。對于需要大量樣品干篩和濕篩的客戶，AS450 basic是即經濟又可靠的選擇。應用實例化學制品, 土壤, 塑料,
Basic－handson－course－in－thermal－analysis－software－(English)
METTLER TOLEDO is pleased to announce their series of one-day training courses, which are designed to familiarize users with thermal analysis theo
Basic－mechanism－of－action－of－PPARa,－PPARb(d)－and－PPARg－and－effects
Similar to other nuclear hormone receptores, PPAR acts as a ligand activated transcription factor. Upon binding fatty acids or hypolipidemic drugs, PP
How－to－use－Basic－Local－Alignment－Search－Tool－(BLAST)
DescriptionThe BLAST algorithm was developed as a way to perform DNA and?protein sequence similarity searches by an algorithm that is?faster than FAST
碳酸復紅（Basic－Fuchsin）顯示X染色質法
實驗方法原理在間期細胞核中，女性X染色質和男性Y染色質均可用特殊染色法顯示出來。女性的兩個X染色體中的一個，在間期時的染色質呈異固縮（Heteropyconosis），呈深染的小體稱Barr氏體。Barr氏體位于間期細胞核內面，呈三角形或半月形小體，易為碳酸復紅或硫堇等染料著色。正常女性Barr氏體
Quantifying－Kinetics－of－Net－Ion－Fluxes－from－Plant－Tissues－by－Noninvasi...
Non-invasive microelectrode ion flux measuring (the MIFE system) allows concurrent quantification of net fluxes of several ions with high spatial
pSEAP2Basic載體的基本信息和質粒圖譜
pSEAP2-Basic載體載體基本信息載體名稱pSEAP2-Basic載體抗性Ampicillin載體長度4677 bp載體類型Basic Cloning Vectors載體來源Clontech拷貝數High copy numberpSEAP2-Basic載體質粒圖譜
Inhibition－of－the－fast－and－slow－blocks－to－polyspermy
Objective: to test the effects of inhibiting the fast and slow blocks to polyspermy on sea urchin development.BackgroundSea urchin eggs release compon
Circadian－Rhythms
Organisms from flies to humans have daily circadian rhythms entrained with the 24-hour cycle of day and night that regulate many physiological systems
兩位諾獎得主、施一公、謝曉亮齊聚2018－SAPA中國年會
分析測試百科網訊 12月1日，“北京昌平?國際生物醫藥產業創新論壇——中國生物醫藥創新的機遇與挑戰暨2018 SAPA中國年會”在北京中關村生命科學園隆重召開。2004年諾貝爾化學獎得主Dr. Aaron J.Ciechanover、2002年諾貝爾化學獎得主Dr. Kurt Wüthrich、
BASIC和AT683兩種半自動生化分析儀的故障維修
???AT638和法國西克曼公司的BASIC兩種半自動分析儀的故障維修?法國西克曼公司的BASIC半自動生化分析儀是一種常見的。它采用的是全息光藕蠓止餼卣第焦韞極管檢測系統。故障現象1：開機機器長鳴報警。???? 在機器設置中，若設置是外置打印機打印，則必須先開打印機，后開主機，使主機自檢時能檢測到