Isolationandcharacterizationofratglomerularendothelialcells
Glomerular endothelial cells (GECs) from the kidney are in close juxtaposition to other cell types, such as mesangial cells, and may respond to as well as release different cytokines that subsequently influence the function of mesangial cells. Weak proliferation of GECs has been described in various types of glomerulonephritis and in transplant rejection. In addition, experimentally induced gl......閱讀全文
Isolation－and－characterization－of－rat－glomerular－endothelial－cells
Glomerular endothelial cells (GECs) from the kidney are in close juxtaposition to other cell types, such as mesangial cells, and may respond to as
Isolation－and－culture－of－rat－coronary－microvascular－endothelial－cells
CMVE were isolated from Wistar rat hearts by digestion of primary cell isolation kit.1.?Hearts mounted on a Langendorff apparatus were perfused at 3
Isolation－of－lymphatic－endothelial－cells
Dermal Cell Suspensions???????????1.?Dermatomed 0.8-mm split-thickness skin was obtained from adult healthy individuals undergoing elective surgery. 2
Isolation－of－lymphatic－endothelial－cells
實驗概要This protocols provides a general protocol for isolation of lymphatic endothelial cells.實驗步驟Dermal Cell Suspensions1.?Dermatomed 0.8-mm split-thic
PriCells:－Isolation－of－endothelial－progenitor－cells－(EPCs)
?PriCells: Isolation of endothelial progenitor cells (EPCs)???1.?Twenty-four mL venous blood was collected at each time point into Vacutainer CPT Mono
Isolation－of－human－corneal－endothelial－cells－(HCECs)
Isolation of HCECs1.?The corneoscleral tissues were rinsed three times with primary cell culture system containing 50 mg/mL gentamicin and 1.25 mg
Isolation－and－cultivation－of－endothelial－progenitor－cells－(EPCs)
Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue regeneration and substantially contri
Isolation－and－longterm－cultivation－of－Human－Corneal－Endothelial－Cells
Coating of the Culture Dish SurfacesCulture dishes were incubated with a film of a 1:1 mixture of laminin (10 μg/ml and chondroitin sulfate (10 μg
Isolation－and－culture－of－SpragueDawley－rat－aortic－smooth－muscle－cells
The intact mature arterial media is composed of at least four phenotypically unique cell subpopulations that reside in distinct medial layers.? Th
細胞組織消化常用的幾種酶的選擇
直接從生物體獲取的組織，一般需要將其消化成單個細胞才能進行體外培養。這種直接從離體組織獲得的細胞，更接近于生物體內的生活狀態，且生物性狀尚未發生很大改變，因此在藥物篩選、細胞移植、類器官培養、腫瘤研究等眾多領域備受歡迎。但組織消化過程中常遇到多種問題，例如消化不完全、細胞死亡率高等。如何克服這些問題
Isolation,－Culture,－Characterization－of－Cortical－and－Hippocampal－Neurons
實驗概要The ?ability to culture primary neurons under serum-free conditions ?facilitates tighter control of neuronal studies. Some serum-free media ?and s
Human－thyroid－endothelial－cell－isolation
1.?Human thyroid glands were obtained at surgery from multinodular goiters.2.?The tissue was incubated in 0.1% type II collagenase (Worthington) overn
Culture－of－retinal－endothelial－cells－and－pericytes
Isolation of retinal microvessels1.?Eyes were obtained and transported on ice.2.?Eyes were cut circumferentially 3 mm posterior to the limbus, the v
Peripheral－blood－“endothelial－progenitor－cells”
EPC Isolation and Characterization1.?EPCs were obtained by isolating mononuclear cells using Ficoll density-gradient centrifugation of human blood buf
Isolation－of－papillary－cells
實驗概要This protocols provides a general protocol for isolation of papillary cells.實驗步驟Isolation of renal papillary cells1.?For ?isolation of papillary c
Isolation－of－papillary－cells
Isolation of renal papillary cells1.?For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/
Isolation－of－rat－cardiac－fibroblasts－and－cardiomyocytes
1.?Hearts were removed from newborn rats (day 0), put into calcium- and bicarbonate-free HEPES-buffered Hanks’ medium, cut into pieces and digeste
ex－vivo－expanded－endothelial－progenitor－cells
Cell Culture. 1.?Total hPBMCs were isolated from blood of human volunteers by density gradient centrifugation. 2.?Cells were plated on culture dishes
Rat－urinary－bladder－urothelial－cells
1.?Bladders were excised from deeply anesthetized (urethane, 1.2 gm???kg?1, i.p.) Sprague Dawley rats (of either sex), cut open, and gently stretc
Isolation－of－rodent－pancreatic－β－cells
1.?Adult rats weighing 250-350g were anesthetized, sacrificed and immediately used for pancreas sampling.2.?Rat islets were isolated from male wistar
Intestinal－intraepithelial－lymphocytes
Intestinal intraepithelial lymphocytes????Intestinal intraepithelial lymphocytes (IELs) are mostly T cells, which are continuously associated with gut
Culturing－Rat－Fetal－Neural－Stem－Cells
實驗概要Rat ?neural stem cells (NSCs) serve as a well-established model for ?investigating human brain development, disease processes, and treatment ?stra
Isolation－of－Human－Pulmonary－Epithelial－Cells
?Isolation of Human Pulmonary Epithelial Cells????1.?Pulmonary epithelial cells from human lung tissue were isolated.2.?Lungs were perfused with 10
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous SystemScott R. Hutton?and?Larysa H. Pevny1UNC Neuroscience Center
Isolation－of－normal－mammary－epithelial－cells
1.?A small piece of tissue from each specimen was removed and minced. 2.?The tissue was digested with collagenase overnight. 3.?To remove the collagen
Isolation－and－culture－of－porcine－muller－cells
1.?Porcine eyes were harvested from pentobarbital-anesthetized animals, each weighing 30 to 40 pounds, and transported to the laboratory in ice-co
Isolation－of－human－endometrial－epithelial－cells
Tissue collection1.?Endometrial biopsies were collected from women undergoing gynaecological procedures for benign conditions. 2.?All women reported
Isolation－of－human－atrial－or－ventricular－cells
1.?Human tissue was derived from atrial or ventricular biopsy specimens belonging to patients undergoing heart surgery.2.?Isolated myocardial tissue w
Isolation－of－human－prostatic－epithelial－cells
1.?A small piece of tissue from each specimen was removed and minced. 2.?The tissue was digested with collagenase overnight. 3.?To remove the collagen
Isolation－and－culture－of－pancreatic－stellate－cells
Pancreatic stellate cells (PaSCs or PSCs) are myofibroblast-like cells found in the areas of the pancreas that have exocrine function.? PaSCs are