ProtocolforTrichloroaceticAcid(TCA)Precipitation
IntroductionThe efficiency of nucleotide incorporation in DNA/RNA polymerization reactions (e.g. transcription, reverse transcription, and DNA replication) can be determined by trichloroacetic acid (TCA) precipitation. TCA precipitates nucleic acid polymers longer than ~20 nucleotides and can therefore be used to separate radiolabeled nucleotides incorporated into nucleic acid from unincorporated label.The following ......閱讀全文
Protocol－for－Trichloroacetic－Acid－(TCA)－Precipitation
IntroductionThe efficiency of nucleotide incorporation in DNA/RNA polymerization reactions (e.g. transcription, reverse transcription, and DNA replica
Protocol－for－Trichl...
實驗概要The ?efficiency of nucleotide incorporation in DNA/RNA polymerization ?reactions (e.g. transcription, reverse transcription, and DNA ?replication)
Protocol－for－Protein－Extraction－for－proteomics
Protocol for Protein Extraction10 % w/v TCA/ acetone/ 0.07 % v/v -MercaptoethanolPlant cells are rich in compounds that interfere with the 2DE separat
DNA抽提
DNA抽提（主要內容如下）·???Working with DNA·???DNA Extraction from Bacteria and Other Organisms·???DNA Extraction from Cell and Tissue·???Mitochondria DNA Isola
James－Hardwicks－angiotensin－assay－protocol
?This specific procedure was developed to assay the activity of the Lck kinase expressed from a retroviral vector in rat fibroblasts. You can obviousl
Angiotensin－Protein－Kinase－Assay
James Hardwick's angiotensin assay protocolThis specific procedure was developed to assay the activity of the Lck kinase expressed from a retrovir
RNA提取
RNA提取（主要內容如下）Tips for Handing RNA?Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum??Basic Procedures for Handing
How－to－prepare－Molecular－Biology－grade－glycogen
OverviewGlycogen??can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be p
制備分子生物學級的糖原
Glycogen??can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased
Acetone－precipitation－of－protein
This procedure is suitable for recovering proteins from most aqueous solvents and from SDS containing buffers. It is not recommended for proteins diss
Maxiprep－preparation－of－Plasmid－DNA
實驗概要The ?PureLink? HiPure Plasmid DNA Maxiprep Kit allows purification of ?500–850 μg of high-quality plasmid DNA from 100–200 mL overnight E. coli cu
蛋白質提取和純化
蛋白質提取和純化（主要內容如下）Protein Extraction?Protein PurificationProtein PrecipitationColumn PreparatioinQ & A Posted in the Method ForumProtein ExtractionWhole
Midiprep－preparation－of－Plasmid－DNA
實驗概要The ?PureLink? HiPure Plasmid DNA Midiprep Kit allows purification of ?100–350 μg of high-quality plasmid DNA from 15–25 mL overnight E. coli ?cul
PEG－PRECIPITATION－OF－PCR－PRODUCTS
PEG PRECIPITATION OF PCR PRODUCTSThis protocol can be used instead of EXO/SAP for removing excess primers and nucleotides from PCR products before cyc
關于三氯乙酸鈉的基本介紹
三氯乙酸鈉是一種化學物質，分子式是C2HCl3O2。是生產某種農藥的主要原料； 在乙烯砜型活性染料印花工藝中作抗酸劑，是生產某種農藥的主要原料 ，產生二氯卡賓，醛的三氯甲基化。 中文名稱：三氯乙酸鈉 英文名稱：Trichloroacetic acid, sodium salt 英文別名：S
重組DNA的分離、克隆與測序實驗手冊
?edited by??????????????? Bruce A. Roe??????????????? Judy S. Crabtreeand Akbar S. KhanDepartment of Chemistry and Biochemistry??????????????? The Uni
Arachidonic－Acid－Labeling
1) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum.2) Pellet cells and wash 1 time with room temperature PBS.3) Resuspend
Amino－acid－composition
There has been a recent revival of interest in the use of AA composition for the identification of proteins from 2-D gels. This technique uses the idi
The－Citric－Acid－Cycle
The Krebs cycle, also called the citric acid cycle, is a fundamental metabolic pathway involving eight enzymes essential for energy production through
Neutralizing－Arachidonic－Acid
NOTE - Use arachidonic acid from Biomol (# FA-003)1) Make up the appropriate concentration of AA in 100% ethanol.2) Add 1μl of phenol red solution.-->
寡核苷酸的相關操作
In this section, you will find techniques related to oligonucleotides, such as oligo purification by acrylamide gel, annealing two oligos to make doub
反向微柱的準備Preparation－of－ReversedPhase－Microcolumns
INTRODUCTIONOne versatile strategy for sample cleanup prior to MALDI-MS analysis uses microscale columns designed for direct sample elution onto the M
Phenol/chloroform－extraction
General InformationPhenol/chloroform extraction is an easy way to remove proteins from your nucleic acid samples and can be carried out in a manner th
RNA－Isolation－Protocol
RNA Isolation Protocol(Revised 5-15-2003)Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109?cells (OD600= 0.2 Dilute cells or scale up)
Oxidation－of－Polyunsaturated－Fatty－Acid
Unsaturated fatty acids are those that contain one or more double bonds in their alkyl-chain. Polyunsaturated fats with two double bonds usually have
DAPI－Nucleic－Acid－Stain
實驗概要The ?blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it ?appears to associate with AT clusters in the minor groove. Binding
Preparation－of－nucleic－acid－probes
Preparation of nucleic acid probesIn standard nucleic acid hybridization assays the probe is labeled in some way. Nucleic acid probes may be made as s
Principles－of－nucleic－acid－hybridization
Principles of nucleic acid hybridization5.2.1.?Nucleic acid hybridization is a method for identifying closely related nucleic acid molecules within tw
PIC－Crosslinking－and－Immune－Precipitation
References?Fishburn et. al., 2005, Molecular Cell, vol. 18 #3: Experimental Procedures pg. 376Immobilized Template assay (Hahn lab website)NotesDTT mu
沉淀反應技術(Precipitation－reaction－technique)
一、???? 概述?可溶性抗原（如細菌浸出液、含菌病料浸出液、血清以及其他來源的蛋白質、多糖質、類脂體等）與其相應的抗體相遇后，在電解質參與下，抗原抗體結合形成白色絮狀沉淀，出現白色沉淀線，此種現象稱為沉淀反應。沉淀反應中的抗原叫沉淀原（precipitinogen）,與沉淀原發生反應的抗體稱為沉淀