HowtoprepareMolecularBiologygradeglycogen
OverviewGlycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased from a number of vendors, the main disadvantage is that it is very expensive (e.g., About 100 dollars/20-40 mg).Here we present a simple and inexpensive protocol to prepare a large amount of glycogen which is suitable for any kind of Molecular Biology......閱讀全文
How－to－prepare－Molecular－Biology－grade－glycogen
OverviewGlycogen??can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be p
制備分子生物學級的糖原
Glycogen??can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased
RNA提取
RNA提取（主要內容如下）Tips for Handing RNA?Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum??Basic Procedures for Handing
浙大，清華發表Nature－Structural－－Molecular－Biology文章
生物通報道：來自浙江大學生命科學研究院，清華大學的研究人員報道了兩個前所未有的GspD通道冷凍電鏡結構，為理解胰泌素超家族，以及II類分泌系統底物運輸的機制提供了一個結構基礎。這一研究成果公布在1月9日的Nature Structural & Molecular Biology雜志上。 在革蘭
Gel－Electrophoresis－of－DNA
What is Electrophoresis?Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. In this CyberLab we
中科院學者Nature－Structural－－Molecular－Biology發表最新成果
10月23日，中國科學院生物化學與細胞生物學研究所國家蛋白質科學中心（上海）許琛琦研究組與牛津大學Omer Dushek研究組合作，在Nature Structural & Molecular Biology上在線發表了題為“Dynamic regulation of CD28 conforma
Nature?Structural?Molecular?Biology揭示細菌脂多糖跨膜轉運機理
4月10日，《自然-結構與分子生物學》（Nature Structural & Molecular Biology）在線發表了中國科學院生物物理研究所研究員黃億華課題組的研究論文Structural basis for lipopolysaccharide extraction by ABC t
DNA的酶學操作
DNA的酶學操作DNA Modifying Enzymes?(Michael Blaber)Introduction to bacterial restriction/modification system. It provides very useful background knowledge
DNA電泳
DNA電泳（主要內容如下）??Preparation of Agarose Gel and Electrophoresis??Extraction of DNA From Agarose Gel??Extraction of DNA from Acrylamide Gels??DNA Marker?
How－to－interrupt－scintillation
Peter Novick Lab, Department of Cell Biology Yale University School of Medicine HOW TO PERFORM AN INTERRUPT OF ANONGOING AUTOCOUNT1). This program int
DNA體外轉染試劑_如何準備轉染所需的質粒DNA
轉染效率受到諸多因素的影響，除了細胞、培養基和載體等影響因素外，另外一項非常重要的因素便是DNA的質量。為了比較不同廠家的轉染效率，我們分別從三家不同的供應商購買了質粒制備試劑盒來制備pEGFP-N3質粒DNA，并通過不同的方法將制備的pEGFP-N3質粒轉運至NIH-3T3細胞。pEGFP-N3質
RNAi－protocol
?siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western
NAi－protocol
siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western
How－to－build－a－BAC－library
Introduction???The?most?important?aspect??of?our?cloning??vectors?is?that?they?are based?on???the?E.?coli?F-factor???replicon.?It?allows?for??strict?
Functional－Genomics－and－Structural－Biology－in－the－Definition－of－Gene...
By mid-2007, the three-dimensional (3D) structures of some 45,000 proteins have been solved, over a period where the linear structures of millions
How－Progesterone－Initiates－Oocyte－Membrane
Progesterone (Pg) binds to both intracellular iPR and plasma membrane- bound mPR. (Right Top) After binding to Pg, iPR is recruited to the membrane as
How－to－Make－Simple－Solutions－and－Dilutions
1. Simple Dilution (Dilution Factor Method based on ratios)A simple dilution is one in which a unit volume of a liquid material of interest is combine
Selfcircularization－of－Linear－DNA
In a microcentrifuge tube prepare a solution of linear DNA (25-50ng) in deionized water or TE buffer (10-35μl).Add:10X ligation buffer?5μl,50% PEG 400
The－E.Z.N.A.?－MagBind?－Dye－terminator－Removal－Procedure
實驗概要Excess ?unincorporated, nonradioactive label can cause high background ?fluorescence in automated sequencing gels. For optimal sequencing ?results
標準PCR
What's?PCR??(Michael Blaber's Lab)Illustrated introduction to usr/localious aspects of PCR technique. It's very valuable not only for thos
標準PCR
·?????????What's PCR??(Michael Blaber's Lab)Illustrated introduction to usr/localious aspects of PCR technique. It's very valuable not onl
Developmental－Effects－of－Transplantation－of－Cell
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mos
How－do－you－synthesize－your－dsRNA
We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends (I. Primer Desig
HOW－TO－USE－THE－COULTER－COUNTER－TO－COUNT－CELLS
1) Turn on the counter by pulling out the on/off button. You need to do this at least 10 min before use to obtain sufficient vacuum.Usually put 0.2 ml
Molecular－Weight－Marker
Molecular Weight Markerl?HindIII Digest Marker SolutionDigest 20 μg l DNA (40 μl DNA if at 0.5 μg/μl)Add 50 μl 10X Tracking DyeBring volume of the dig
Microscopes－in－Cell－Biology
Microscopes in Cell BiologyIntroductionMicroscopy has a major role in the study of cells. From the very beginning, researchers have tried to develop w
早期胚胎發育中的單胚胎細胞基因表達（一）
Single-embryo Gene Expression for Early Embryo DevelopmentMylene Yao, M.D. Assistant ProfessorDept. of Obstetrics and Gynecology Stanford UniversityMy
Fluorescent－Nucleoside－Triphosphates－for－SingleMolecule－Enzymology1
By:?Christopher P. Toseland1 2?,?Martin R. Webb1Affiliation(s):?(1)?MRC National Institute for Medical Research, London, UK(2)?Institut für Zellul?re
Cellular－－Molecular－Pathology－Branch
VisionTo provide scientific collaboration of excellence to National Toxicology Program (NTP)?(http://ntp.niehs.nih.gov/)?interdisciplinary research pr
Molecular－Analysis－and－Results－－DNA
Theory of CGHComparative genomic hybridization (CGH) is a fairly new molecular cytogenetic technique that allows detection of DNA sequence copy number