CellSurfaceImmunofluorescenceStainingProtocol
實驗概要A method of identifying and enumerating specific cell types in a heterogeneous population of cells by enhancing the specific staining of desired cells, comprising contacting a sample from the heterogeneous population of cells with a labeled primary antibody which recognizes and binds to a desired cell surface antigen and an unlabeled cross-linking agent which recognizes and bin......閱讀全文
Intracellular－Staining－Protocol
1.?Fix cells- Add16% formaldehyde directly into culture medium to obtain a final concentration of 1.5% formaldehyde.2. Incubate in fixative for 10 min
Silver－Staining－Protocol
1x 40min - overnight?????50% MeOH, 12% Acetic Acid1x 30min??????????????????????????????????50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde3x 20min?
Alkaline－phosphatase－staining
4.5.1.1 General informationEndothelial cells possess an endogenous alkaline phosphatase (AP) activity. The enzymatic activity of AP is not restricted
Fluorescent－Staining－of－Cells
1. Fluorescent phalloidin in methanol. Phallacidin does not work as well. Dilute 10 ul 330 nM stock into 500 ul PBS for each large coverslip.2. PB
Protein－Staining－Procedures
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
Staining－Methods－for－cell
death Z. Xia 10/2/95The simplest way: trypan blue.?Dead cells stain blueNon-fixed cells: FDA(fluorescein diacetate)-green, alive cells;?P.I. (propidiu
Wholemount－staining－of－embryos
Fix embryos in formalin or MEMFA for one hour at room temperature with mixing. Rinse with TBS, replace with methanol, store at -20oC.Rehydrate by slow
Gramstaining－Procedure
Gram-staining is a four part procedure which uses certain dyes to make a bacterial cell stand out against against its background. The specimen should
Direct/Indirect－Staining－Protocol
Use this Protocol for Directly or Indirectly staining cells for Flow Cytometric Analysis1) Dilute cells to 5x10^6 cells/mL2) Aliquot 100uL of cells pe
High－resolution－negative－staining
High resolution negative staining(From Valentine et al, 1968. Biochemistry 7:2143-52)Rationale:?For the highest resolution with negative staining, the
Staining－Methods－for－cell－death
The simplest way: trypan blue.?Dead cells stain blueNon-fixed cells: FDA(fluorescein diacetate)-green, alive cells;?P.I. (propidium iodide)-red, dead
Preparation－and－Staining－of－Paraffin－Sections
I. Fixation and Processing of Tissue for Paraffin SectionsA. Fixation of Tissues in 10% Neutral Buffered FormalinSacrifice animal by prescribed and ap
Blood－Smear:－Preparation－and－Staining
Blood Smear: Preparation and StainingReference:Davidson, I. and Henry J., Clinical Diagnosis by Laboratory Methods, I. Davidsohn and J. Henry, eds., W
Intracellular－Cytokine－Staining－Protocol
實驗概要A ?modification of the basic immunofluorescence staining and flow ?cytometric analysis protocol can be used for the simultaneous analysis ?of surf
Actin－StainingActin－Staining－Protocol
實驗概要Invitrogen ?offers several fluorescent and biotinylated phalloidin and phallacidin ?derivatives for labeling F-actin. These phallotoxins, isolated
Preparation－and－Staining－of－Frozen－Tissue－Sections
I. Preparation of Frozen Sections for SectioningMaterials neeed:2-methylbutane (isopentane)Liquid NitrogenDry icePeel-Away?/sup> base moldsFrozen tiss
Immunofluorescent－Staining－of－Mouse－and－Rat－Leukocytes
I. ProcedureHarvest cells from tissue, preparing a single cell suspension. Red blood cells may be removed by lysis or density gradient: Red blood cell
Cell－Surface－Immunofluorescence－Staining－Protocol
實驗概要A method of identifying ?and enumerating specific cell types in a heterogeneous population of ?cells by enhancing the specific staining of desired
Immunofluorescent－staining－of－Sea－Urchin－embryos
1. Transfer fixed embryos to microfuge tubes. Allow to settle for 10 minutes.Gently remove most of the liquid.2. Add 100 ul antibody to one tube and 1
SSR－GEL－and－Silver－Staining－Protocol
I.?EQUIPMENT:DNA sequencing unit (35 x 45 cm) & 2000V power supplyClampsLg. plastic trays (4), about 43 x 50 x 8 cm, and one lidTwo rocking platformsH
Methylene－Blue－DNA－staining－protocol
Methylene Blue DNA staining protocolProtocol:Load 2-5X the amount of DNA that would give bands of moderate intensity on an ethidium bromide stained ge
Intracellular－Immunofluorescent－Staining－for－Flow－Cytometry
IntroductionA modification of the basic immunofluorescent staining and?flow cytometric analysis protocol?can be used for the simultaneous analysis of
Cell－Cycle－Staining－ProtocolDAPI
1. Harvest cells- wash 2X in PBS to get rid of serum proteins. 1200rpm, 5 min2. Resuspend pellet (up to 3x106 cells) in 1.2 ml PBS (Ca and Mg free).3.
FIXATION－and－DNA－Staining－for－Cell－Cycle－Analysis
BackgroundThis method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to ente
Protocol－for－intracytoplasmic－staining－of－cytokines－for－FACS－analysis
DescriptionProtocol for intracytoplasmic staining of cytokines for FACS analysis?Procedure1) Prepare spleen, lymph node or T cell clone cells as singl
Immunofluorescent－Staining－of－Drosophila－Larval－Brain－Tissue
INTRODUCTIONThe?Drosophila?larval brain is a well-established model for?investigating the role of stem cells in development. Neuroblasts?(neural stem
細菌的芽孢染色(spore－staining)
實驗原理細菌的芽胞具有厚而致密的壁，透性低，不易著色，若用一般染色法只能使菌體著色而芽胞不著色（芽孢呈無色透明狀）。芽孢染色法就是根據芽孢既難以染色而一旦染上色后又難以脫色這一特點而設計的。所有的芽孢染色法都基于同一個原則：除了用著色力強的染料外，還需要加熱，以促進芽孢著色。當染芽孢時，菌體也會著色
Preparation－of－fixed－embryos－for－immunocytochemistry－and－AP－staining
1. Transfer 50 ml of embryo cultures to centrifuge tubes. Spin at 1500 rpm for 5 minutes. Check that you can see a pellet of embryos at the bottom.Qui
Immunofluorescent－Staining－of－Drosophila－Larval－Brain－Tissue
實驗概要The Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem cells)
Staining－Procedure－for－Flow－Cytometric－Detection－of－Human－Cyclins
Staining Procedure for Flow Cytometric Detection of Human CyclinsThis is a standard protocol used at Pharmingen for Quality Control testing of the ant