GelelongationassayfortypeIIfattyacidsynthesis
Gel-elongation assay for type II fatty acid synthesisSrinivas KodaliAndrew GalgociSheo Singh Dr.Jun Wang Dr., jun_wang2@merck.com, Merck Research LaboratoriesRelated Journal & Article InformationManuscript: 2006-01-00990CJournal: NatureArticle Title: Platensimycin is a selective FabF inhibitor with potent antibiotic propertiesIntroductionType II fatty acid synthesis (FASII) is essential to bacterial cell via......閱讀全文
Gelelongation－assay－for－type－II－fatty－acid－synthesis
Gel-elongation assay for type II fatty acid synthesisSrinivas KodaliAndrew GalgociSheo Singh Dr.Jun Wang Dr., jun_wang2@merck.com, Merck Research Labo
凝膠延長分析二型脂肪酸合成的方法
Gel-elongation assay for type II fatty acid synthesisSrinivas KodaliAndrew GalgociSheo Singh Dr.Jun Wang Dr., jun_wang2@merck.com, Merck Research Labo
Oxidation－of－Polyunsaturated－Fatty－Acid
Unsaturated fatty acids are those that contain one or more double bonds in their alkyl-chain. Polyunsaturated fats with two double bonds usually have
全細胞靶點篩選抗生素新藥的方法
A?target-specific?whole?cell?assay?for?antibacterial?drug?discoveryLorraine HernandezSrinivas KodaliDoris CullySheo SinghJun Wang , jun_wang2@merck.co
Primary－type－II－pneumonocyte－Isolation－and－culture
Pulmonary alveolar type II cells carry out highly specialized functions that include the synthesis, secretion, and reutilization of surfactant, a
Assay－of－Phospholipase－A－Activity
Phospholipases of the A type constitute a large family of esterases that catalyze the hydrolysis of the fatty acid ester bonds in phospholipids an
Oxidation－ofoddnumbered－chain－fatty－acid－from－PropionylCoA－toSuccinylCoA
NON-Clickable Image?The beta-oxidation of fatty acids in mitochondria progressively shortens fatty acids two-carbons at a time as acetyl-CoA units are
Regulation－And－Function－Of－ChREBP－in－Liver
Liver is the major site for carbohydrate metabolism (glycolysis and glycogen synthesis) and triglyceride synthesis (lipogenesis). While insulin was lo
Coenzyme－A－Detection
實驗概要The experiment provided ?an ?easy, convenient assay to measure the CoA level in a variety of ?biological samples. In the assay, free CoA is specif
Synthesis－of－plasmalogens
Plasmalogens are a class of glycerophospholipids that contain one vinyl-ester linked long chain alcohol group in place of one ester-linked fatty acid
Lipid－analysis－Week－3:－GAS－LIQUID－CHROMATOGRAPHY
During this week, you will analyze the fatty acid composition of the individual lipid fractions recovered from the TLC plate. Gas chromatography is a
Reversal－of－Insulin－Resistance－by－Leptin
The insulin resistance of type II diabetes appears to be caused in part by the presence of high levels of lipids in cells such as skeletal muscle wher
Sucrose－Synthesis
The prodution of sucrose takes place in plants, to store energy produced during photosynthesis. The extraction of sucrose from plants like sugar cane
Lactose－Synthesis
The synthesis of glycosidic bonds between monosaccharides requires energy input, energy that is provided by joining sugar monomers to a nucleotide suc
Starch－Synthesis
The primary carbohydrate used for energy storage in plants is starch, the equivalent of glycogen in animals. Both starch and glycogen are polymers of
Eicosanoid－Metabolism
The eicosanoids are a family of lipophilic hormones derived from the twenty carbon fatty acid arachidonic acid. Although they are diverse in structure
Microtubule－Binding－Assays
MaterialsSiliconized ultracentrifuge microfuge tubesGTP-depleted microtubules6X SDS loading dye1X SDS loading dyeCoomassie Brilliant Blue R 250 (0.8%
BetaOxidation－of－Fatty－Acids
Fatty acids provide highly efficient energy storage, storing much more energy for their weight than carbohydrates like glucose. Fatty acids are stored
Methylation－of－Fatty－Acids－(Kropinski－Method)
Methylation of Fatty Acids (Kropinski Method)OBJECTIVE:To methylate fatty acids in whole cells or lipopolysaccharide.REAGENTS :Methanol-Hydrochloride
Library－cDNA－Synthesis
Library cDNA Synthesis1° cDNA SynthesisN.B:?During 1° cDNA synthesis, all steps should be carried while wearing gloves and all solutions should either
Synthesis－of－Cardiolipin－－phosphatidylinositol
Phosphoglycerides are lipids that contain glycerol with a phosphate ester on one end and two fatty acid ester side chains. Phosphoglycerides such as p
Labeled－Sphingomyelin－Synthesis
Synthesis reaction:1) Mix purified DMSM with cyclohexylamine and 14CH3I at a ratio of 1/1.1/1.3 in 5 ml of methanol.2) Allow reaction to proceed at ro
Protocol－for－dsRNA－Synthesis
實驗概要? ? ? ? We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends
Comparison－of－Beta－oxidation－in－mitochondria－and－peroxisomes－andglyoxysomes
In addition to the beta-oxidation of fatty acids in mitochondria, animal cells carry out beta-oxidation in another organelle, the peroxisome. Although
The－Citric－Acid－Cycle
The Krebs cycle, also called the citric acid cycle, is a fundamental metabolic pathway involving eight enzymes essential for energy production through
磷脂合成中產生的脂肪酸驅動吞噬泡的擴張
近日，德國科隆大學等科研機構的科研人員在Cell上發表了題為“Local Fatty Acid Channeling into Phospholipid Synthesis Drives Phagophore Expansion”的文章，發現在自噬過程中，磷脂合成中產生的局部脂肪酸驅動吞噬泡的擴
Preparation－of－nucleic－acid－probes
Preparation of nucleic acid probesIn standard nucleic acid hybridization assays the probe is labeled in some way. Nucleic acid probes may be made as s
Glucogenic－and－Ketogenic－Amino－Acids
A significant amount of metabolic energy can come from amino acid metabolism, particularly under conditions of starvation. The metabolism of amino aci
自噬過程中磷脂合成中產生的脂肪酸驅動吞噬泡的擴張
近日，德國科隆大學等科研機構的科研人員在Cell上發表了題為“Local Fatty Acid Channeling into Phospholipid Synthesis Drives Phagophore Expansion”的文章，發現在自噬過程中，磷脂合成中產生的局部脂肪酸驅動吞噬泡的擴
SREBP－control－of－lipid－synthesis
Sterol-regulatory element binding proteins (SREBPs) play a key role in transcriptional regulation of cholesterol metabolism in response to cholesterol