NegativeStainElectronMicroscopyofMicrotubules
Negative staining is a rapid, qualitative method for analyzing microtubule structure at the EM level. Because negative staining involves deposition of heavy atom stains, structural artifacts such as flattening of the cylindrical microtubule and opening up of microtubules into flat sheets are common. Cryo-electron microscopy, where microtubules are flash frozen in a thin film of vitreous ice and imaged without stainin......閱讀全文
Microscopy－with－Oil－Immersion
Microscopy with Oil ImmersionPrincipleWhen light passes from a material of one refractive index to material of another, as from glass to air or from a
Preparation－of－Segmented－and－Polarity－Marked－Microtubules
Preparation of Segmented and Polarity Marked Microtubules?Segmented and polarity-marked microtubules are very useful for many different types of?in vi
Preparation－of－Segmented－and－Polarity－Marked－Microtubules
Segmented and polarity-marked microtubules are very useful for many different types of?in vitro?assays. Segmented microtubules are microtubules with a
細菌的簡單染色(Simple－stain)與革蘭氏染色(Gram－stain)
一、目的要求1、學習細菌染色的原理和方法；2、掌握細菌的簡單染色法和革蘭氏染色法。二、基本原理用于生物染色的染料主要有堿性染料、酸性染料和中性染料三大類。堿性染料的離子帶正電荷，能和帶負電荷的物質結合。因細菌蛋白質等電點較低，當它生長于中性、堿性或弱酸性的溶液中時常帶負電荷，所以通常采用堿性染料（如
Size－and－Shape－of－Protein－Molecules1
Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron MicroscopyAn important part of ch
Alcian－green－stain－for－cartilage
This is an easy method for stianing cartilage that can be used with isolated or CAM-grafted limbs to study limb development or with whole embryos (8 t
DAPI－Nucleic－Acid－Stain
實驗概要The ?blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it ?appears to associate with AT clusters in the minor groove. Binding
Vybrant?－DyeCycle?－Ruby－stain
實驗概要Live cell studies ?of cellular DNA content and cell cycle distribution are useful to detect ?variations of growth patterns due to a variety of phy
Vybrant?－DyeCycle?－Violet－Stain
實驗概要Live cell studies ?of cellular DNA content and cell cycle distribution are useful to detect ?variations of growth patterns due to a variety of phy
Transmission－Electron－Microscope－(TEM)
所謂TEM，就是一個放大鏡疊加了一臺照相機。這臺放大鏡的放大倍數比較高，可高達一百萬倍。當然，拋開分辨率談放大倍數都是耍流氓，那么，TEM的分辨率有多高呢？答案是 it depends。一般來說，TEM的分辨率要在1到2個納米，STEM更高，但是STEM得成像技術類似于SEM，但用的不是二次電子。我
細胞組分和細胞器——細胞器分離
Labeling Microtubules?(Molecular Dynamics Inc.??)Microtubules are involved in many aspects of cell motion including propulsion, mitosis, growth, and o
Permeabilization－of－gramnegative－bacteria－with－KPi/hexane
Harvest (10,000 rpm for 5 min) 24 h grown (in LB pH 7.5, 37?oC, 200 rpm)?Escherichia coli,?Enterobacter aerogenes?or?Pseudomonas aeruginosa?cells.Wash
Immunofluorescence－/－Confocal－Microscopy－Protocol
實驗概要Immunofluorescence ?is a technique used for light microscopy with a fluorescence microscope ?and is used primarily on biological samples. This tec
Immunofluorescence－/－Confocal－Microscopy－Protocol
實驗概要Immunofluorescence ?is a technique used for light microscopy with a fluorescence microscope ?and is used primarily on biological samples. This tec
Human－Placental－Alkaline－Phosphatase－Stain
The following protocol is for detection of human placental alkaline phosphatase (AP) in cultured cells. Human placental AP is heat stable, unlike othe
AbC?－AntiMouse－Bead－Kit
實驗概要The ?AbC? Anti-Mouse Bead Kit provides a consistent, accurate, and ?simple-to-use technique for the setting of flow cytometry compensation ?when u
Peripheral－blood－“endothelial－progenitor－cells”
EPC Isolation and Characterization1.?EPCs were obtained by isolating mononuclear cells using Ficoll density-gradient centrifugation of human blood buf
HIVI－Nef:－negative－effector－of－Fas－and－TNF
HIV infection leads to drastic declines in CD4 T helper cells, in part through apoptosis of uninfected cells. Apoptosis of uninfected cells may be ind
AbC?－AntiRat/Hamster－Bead－Kit
實驗概要The AbC? ?anti-Rat/Hamster Bead Kit provides a consistent, accurate, and ?simple-to-use technique for the setting of flow cytometry compensation ?
AbC?－AntiRat/Hamster－Bead－Kit
實驗概要The AbC? ?anti-Rat/Hamster Bead Kit provides a consistent, accurate, and ?simple-to-use technique for the setting of flow cytometry compensation ?
Silver:－TimeLapse－Microscopy
Pad Preparation1. Microwave 2% agarose (mix of low-melt and normal, to taste) in Thorn media (see below). (If you have used different percentages of a
Immunofluorescence－Microscopy－of－tissue－culture－cells
Immunofluorescence Microscopy of tissue culture cellsThese methods are written for direct staining of filamentous actin with bodipy FL-phallicidin and
Ultraviolet－irradiation－impairs－epiboly－via－microtubules－in－Zebrafish
IntroductionZebrafish have transparentembryos that develop outside the mother. They develop rapidly, so that at 24 hours after fertilization, the embr
Flow－Cell－Assays－with－Microtubules:－Motility/Dynamics－in－Fluorescence
Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/
常用無機材料分析方法
Elemental Analysis 元素分析Atomic absorption spectroscopy 原子吸收光譜Auger electron spectroscopy (AES) 俄歇電子能譜Electron probe microanalysis (EPMA) 電子探針微分析Electro
LIVE/DEAD?－Fixable－Dead－Cell－Stain－Kits
實驗概要The ?LIVE/DEAD? Fixable Dead Cell Stain Kits use a novel method to evaluate ?the viability of mammalian cells by flow cytometry. These assays are
細菌的莢膜染色(capsule－stain)
實驗原理由于莢膜與染料間的親和力弱，不易著色，通常采用負染色法染莢膜，即設法使菌體和背景著色而莢膜不著色，從而使莢膜在菌體周圍呈一透明圈。由于莢膜的含水量在90%以上，故染色時一般不加熱固定，以免莢膜皺縮變形。 實驗器材 1.活材料 培養3-5天的膠質芽孢桿菌（Bacillus muci
細菌的鞭毛染色(Flagella－stain)
實驗器材1.活材料培養12-16h的水稻黃單胞菌（Xanthomonas oryzae），粘質賽氏桿菌（Serratia marcescens）或假單細胞菌（Pseudomonas sp.）斜面菌種。2.染色液和試劑硝酸銀染色液、Leifson染色液、香柏油、二甲苯。3.器材載玻片、擦鏡紙、吸水紙、
LIVE/DEAD?－Fixable－Dead－Cell－Stain－Kits
實驗概要The LIVE/DEAD? ?Fixable Dead Cell Stain Kits use a novel method to evaluate the ?viability of mammalian cells by flow cytometry. These assays are
Downregulated－of－MTA3－in－ERnegative－Breast－Tumors
Approximately 30% of breast carcinomas lack ER expression. Presumably, these breast cancers become estrogen independent through genetic alterations th