InactivationofGsk3byAKTcausesaccumulationofbcatenin
Lipopolysaccharide (LPS) from XX bacteria induces a wide range of inflammatory responses, including the response of alveolar macrophages to bacteria in the lungs. CD14 and the Toll-like receptor TLR4 are activated by LPS, initiating a signaling cascade that activates PI3 kinase and increases synthesis of the inositol phosphate PIP3 (see Toll-like Receptor pathway). PIP3 activates the inositol phosphate dependent prot......閱讀全文
Inactivation－of－Gsk3－by－AKT－causes－accumulation－of－bcatenin
Lipopolysaccharide (LPS) from XX bacteria induces a wide range of inflammatory responses, including the response of alveolar macrophages to bacteria i
Anthrax－Toxin－Mechanism－of－Action
One of the key causes of anthrax virulence is the production of three specific factors by the gram-positive spore forming bacteria Bacillus anthracis.
Thermal－Inactivation
Thermal InactivationA simple, reversible way to a stop restriction reaction is by adding EDTA, which chelates Mg2+, thereby preventing catalysis. If f
wnt信號通路如何影響神經干細胞增殖
wnt信號通路主要是通過影響b-catenin來發揮作用的，當有wnt信號時，APC，Axin和GSK3的復合物無法形成，主要是GSK3無法與b-catenin結合，使得其在細胞質中積累，進入細胞核，從而啟動癌基因的啟動子密碼，發生癌變。此外還有文獻說是b-catenin除了進入細胞核之外，也可以與
Invitro－Phagocytosis－Assay－of－Macrophages
IntroductionThe term phagocytosis itself describes its mean phage = engulfment; cytosis: cell process. In other words, phagocytosis is the cellular pr
Transfection－of－siRNA－oligos－to－peritoneal－macrophages
Use DeliverX Transfection from PanomicsModified protocol belowStep 1:? Make complexesNote:? Do not scale up more than 3xThaw siRNA and DeliverX reagen
Serum－Thawing－－Heat－Inactivation
Serum Thawing & Heat Inactivation(Chris Cohick from JRH Biosciences catalogue)How to thaw serum:Serum that is stored at -10o C to -40o C is stable for
Serum－Thawing－－Heat－Inactivation
How to thaw serum:Serum that is stored at -10o C to -40o C is stable for extended periods of time. It is neither necessary or desirable to store serum
NO2dependent－IL－12－Pathway－in－NK－cells
Macrophages and NK cells help provide innate immunity against infection by intracellular parasites and communicate with each other to regulate this pr
ALK－in－cardiac－myocytes
Heart formation is cued by a combination of positive and negative signals from surrounding tissues. Inhibitory signals that block heart formation in a
Cadmium－induces－DNA－synthesis－and－proliferation－in－macrophages
Exposure to divalent cadmium ions (Cd2+) is a known cancer risk factor, but the molecular mechanisms responsible for the inappropriate induction of ce
Map－Kinase－Inactivation－of－SMRT－Corepressor
Corepressors are coregulators that interact with transcriptional silencers in a variety of pathways such as cell proliferation, differentiation and ap
賀福初院士Cell－Res解析細胞增殖調控
來自北京蛋白質組研究中心研究人員在新研究中證實，CKIP-1通過抑制TRAF6介導的Akt激活調控了巨噬細胞增殖。 國際著名細胞生物學家、遺傳學家賀福初（Fuchu He）院士和北京蛋白質組研究中心的張令強（Lingqiang Zhang）教授是這篇論文的共同通訊作者。賀福初院士主要從事基因組
賀福初院士Cell－Res解析細胞增殖調控
來自北京蛋白質組研究中心研究人員在新研究中證實，CKIP-1通過抑制TRAF6介導的Akt激活調控了巨噬細胞增殖。 國際著名細胞生物學家、遺傳學家賀福初（Fuchu He）院士和北京蛋白質組研究中心的張令強（Lingqiang Zhang）教授是這篇論文的共同通訊作者。賀福初院士主要從事基因組
Akt與心血管疾病
? 目前心腦血管疾病發病率及死亡率居全球首位，正嚴重威脅人類健康。由此心臟病學專家及臨床工作者十分重視對心腦血管疾病的研究。近年來，從分子生物水平入手對心血管疾病發病機制研究已經取得很大成就。其中細胞信號轉導通路備受關注，Akt作為信號網絡的中樞環節，對細胞增殖、分化和存活起到重要作用，與動脈粥
揭示糖尿病新機制還得從這類修飾入手（二）
02、組學生信分析發現糖尿病小鼠胰島中信號通路重構為了探討調控磷酸化蛋白質組水平變化的機制，作者對db/db胰島中顯著變化的激酶底物motif進行分析（圖2A）。結果顯示，與高營養素誘導mTOR激活一致，p70S6K底物motif顯著富集，而AMPK motif下調表達。其他激酶motif，
Gene－Inactivation－in－the－Cyanobacterium－Synechococcus－sp.－PCC－7002－and...
Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Const
PTEN－dependent－cell－cycle－arrest－and－apoptosis
PTEN is a tumor suppressor gene. Recombinant PTEN is capable of dephosphorylating phosphatidylinositol 3,4,5-triphosphate[PI(3,4,5)P3], the product of
蛋白質組標桿大牛揭示糖尿病新機制還得從這類修飾入手
原文： Phosphoproteomics Reveals the GSK3-PDX1 Axis as a Key Pathogenic Signaling Node in Diabetic Islets 原文鏈接： https://doi.org/10.1016/j.cmet
Cell子刊－－蛋白質組標桿大牛揭示糖尿病新機制還得從這類
關鍵詞：磷酸化蛋白質組、蛋白質組、II型糖尿病、胰島β細胞 原文： Phosphoproteomics Reveals the GSK3-PDX1 Axis as a Key Pathogenic Signaling Node in Diabetic Islets 原文鏈接：
GentleMACS/單細胞懸液制備文獻集錦
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?? ? ? ? ? ? 種屬 處理組織 文章名稱 human ? ? ? ? ? ? ?
Hypoxia－and－p53－in－the－Cardiovascular－system
Hypoxic stress, like DNA damage, induces p53 protein accumulation and p53-dependent apoptosis in oncogenically transformed cells. Unlike DNA damage, h
氧化磷酸化選擇性地調節組織巨噬細胞穩態
西班牙卡洛斯三世國家心血管研究中心David Sancho等研究人員合作發現，氧化磷酸化選擇性地調節組織巨噬細胞穩態。這一研究成果于2023年2月3日在線發表在國際學術期刊《免疫》上。通過分析人類和小鼠的RNA-seq數據，研究人員確定氧化磷酸化（OXPHOS）是不同器官組織巨噬細胞（TMF）在穩態
AKT－Signaling－Pathway
Many cell-surface receptors induce production of second messengers like PIP3, phosphatidylinositol 3,4,5-trisphosphate, that convey signals to the cyt
JEM：能移除細胞碎片的受訓免疫細胞或有望幫助解決機體的肺部炎癥
炎癥是機體免疫系統反應的一個標準部分，但有時這種反應在肺部卻會變得過度活躍，從而導致炎癥持續不可控制，而這或許是致命性的。許多死于COVID-19的人群都是因過度炎癥所導致的急性肺部損傷所致。近日，一篇發表在國際雜志Journal of Experimental Medicine上題為“Trai
A20酶活性通過促進轉錄因子C/EBPβ降解抑制肺纖維化
肺纖維化嚴重影響呼吸功能，表現為干咳、進行性呼吸困難（自覺氣不夠用），且隨著病情和肺部損傷的加重，患者呼吸功能不斷惡化。特發性肺纖維化發病率和死亡率逐年增加，診斷后的平均生存期僅2.8年，死亡率高于大多數腫瘤，被稱為一種“類腫瘤疾病”。 科學家們認為，肺纖維化既是獨立疾病，也是慢性纖維增生性
大鼠磷酸化AKT蛋白(AKT)ELISA檢測法
大鼠磷酸化AKT蛋白(AKT)ELISA試劑盒?(用于血清、血漿、細胞培養上清液和其它生物體液內)?原理本實驗采用雙抗體夾心?ABC-ELISA法。用抗大鼠?AKT?單抗包被于酶標板上，標準品和樣品中的AKT與單抗結合，加入生物素化的抗大鼠AKT，形成免疫復合物連接在板上，辣根過氧化物酶標記的Str
Skeletal－muscle－hypertrophy－is－regulated－via－AKT/mTOR－pathway
Skeletal muscle atrophies with disuse while with increased use and increased load skeletal muscle exhibits hypertrophy, with an increase in the size o
RASSF1基因編碼的功能和結構描述
這個基因編碼一種與RAS效應蛋白相似的蛋白質該基因的缺失或表達改變與多種癌癥的發病有關，提示該基因具有抑癌作用該基因的失活與cpg島啟動子區的高甲基化有關。編碼蛋白與DNA修復蛋白XPA相互作用該蛋白還可以抑制細胞周期蛋白d1的積累，從而誘導細胞周期停滯。已經報道了該基因編碼不同亞型的幾種選擇性剪接
How－Progesterone－Initiates－Oocyte－Membrane
Progesterone (Pg) binds to both intracellular iPR and plasma membrane- bound mPR. (Right Top) After binding to Pg, iPR is recruited to the membrane as