GeneInactivationintheCyanobacteriumSynechococcussp.PCC7002and...
Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural TransformationInactivation of a chromosomal gene is a useful approach to study the function of the gene in question and can be used to produce a desired phenotype in the organism. This chapter describes how to generate such mutants of the cyanoba......閱讀全文
Gene－Inactivation－in－the－Cyanobacterium－Synechococcus－sp.－PCC－7002－and...
Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Const
Thermal－Inactivation
Thermal InactivationA simple, reversible way to a stop restriction reaction is by adding EDTA, which chelates Mg2+, thereby preventing catalysis. If f
Serum－Thawing－－Heat－Inactivation
Serum Thawing & Heat Inactivation(Chris Cohick from JRH Biosciences catalogue)How to thaw serum:Serum that is stored at -10o C to -40o C is stable for
Serum－Thawing－－Heat－Inactivation
How to thaw serum:Serum that is stored at -10o C to -40o C is stable for extended periods of time. It is neither necessary or desirable to store serum
Map－Kinase－Inactivation－of－SMRT－Corepressor
Corepressors are coregulators that interact with transcriptional silencers in a variety of pathways such as cell proliferation, differentiation and ap
基因轉型(gene－transformation)
目的帶有特定基因的質體在分子生物研究上，是一個很重要的工具，將質體送入細菌的過程稱為基因轉形，經由基因轉型可使質體在細菌中大量復制，以備進一步研究。本實驗將把你在前面轉殖實驗中cDNA和質體DNA的連結反應送入細菌。 你將從本實驗學習如何進行基因轉型作用。原理早在1970年左右，有人發現細菌經由冰冷
Use－of－the－GUS－Reporter－Gene
One of the most important considerations in the expression of heterologous proteins in plants is the choice of promoter. The study of promoter act
Gene－Structure－Annotation－at－PlantGDB
The accurate identification of exons and introns that comprise a complete plant gene structure can be a time-consuming and challenging task. Novel
Inactivation－of－Gsk3－by－AKT－causes－accumulation－of－bcatenin
Lipopolysaccharide (LPS) from XX bacteria induces a wide range of inflammatory responses, including the response of alveolar macrophages to bacteria i
FOSB－gene－expression－and－drug－abuse
Drug addiction is associated with long-term behavioral changes, suggesting a long-lived transcriptional regulator that responds to chronic drug exposu
Reverse－Transfection－for－Gene－Function－Analysis
This guide describes a microarray-based system for the functional analysis in mammalian cells of many genes in parallel. Mammalian cells are cultured
差異基因表達研究方法介紹（DDPCR；GENEFISHING；GENE－CHIP）
差異基因表達的研究受到了廣泛的關注，常用的技術有DD-PCR；GENE-FISHING；GENE CHIP等。簡單介紹如下： DDRT -PCR技術即mRNA差異顯示聚合酶鏈式反應技術，此技術是以PCR技術和聚丙烯凝膠電泳技術為基礎，結合銀染或放射性自顯影等顯色技術，能快速有效地
PCR－Primers－For－Gene－Expression－Detection－or－Quantification
Why PrimerBank?PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time
Double－Stranded－RNA－Induced－Gene－Expression
One defense against viral infection is provided by PKR, double-stranded RNA activated protein kinase. When PKR interacts with dsRNA found in cells dur
Yeast－Gene－knockout－using－Oligo/PCR
Universal primers for gene knock-out using dominant drug markers: Kan, Clonat, and Hygromisin-B.?Forward primer: 5’ TCAGGGGCATGATGTGACT 3’Reverse prim
Control－of－Gene－Expression－by－Vitamin－D－Receptor
The vitamin D receptor, VDR is the mediator of all genomic actions of vitamin D3 and its analogs. It belongs to a family of ligand induced transcripti
Functional－Genomics－and－Structural－Biology－in－the－Definition－of－Gene...
By mid-2007, the three-dimensional (3D) structures of some 45,000 proteins have been solved, over a period where the linear structures of millions
基因芯片(gene－chip)的原理
基因芯片(gene chip)的原型是80年代中期提出的。基因芯片的測序原理是雜交測序方法,即通過與一組已知序列的核酸探針雜交進行核酸序列測定的方法,在一塊基片表面固定了序列已知的八核苷酸的探針。當溶液中帶有熒光標記的核酸序列TATGCAATCTAG,與基因芯片上對應位置的核酸探針產生互補匹配時,通
Targeted－Gene－Replacement－in－Fungal－Pathogens－via－Agrobacterium－...
Genome sequence data on fungal pathogens provide the opportunity to carry out a reverse genetics approach to uncover gene function. Efficient meth
報告基因(report－gene)的應用
報告基因被廣泛地應用于細胞生物學中的基因表達和細胞相關內容的研究。常用的報告基因主要有GUS基因、CAT基因、hGH基因、Cato2ase基因、綠色熒光蛋白基因及螢火蟲熒光素酶基因等。在這些報告基因中螢火蟲熒光素酶基因因其表達產物－熒光素酶敏感性高，測定方法快速且宜于掌握，線性好，并且熒光素酶產生的
GENEπ數字PCR技術應用教程
數字 PCR( digital PCR ，dPCR ) 下一代DNA/RNA擴增技術。原理是將一個PCR 反應體系分配到大量微小的反應單元中，在每個微反應器中包含或不包含 1 個或多個拷貝的目標核酸分子 (DNA 模板) ，進行“單分子模板”PCR 擴增。擴增結束后，通過陽性反應單元( 通過
Overview－of－telomerase－protein－component－gene－hTert－Transcriptional
Telomerase is an enzyme which replicates the terminal sequences of eukaryotic chromosomes, namely the telomeres. Cells which have an unlimited replica
基因治療按基因操作分類介紹
一類為基因修正（gene correction）和基因置換（gene replacement），即將缺陷基因的異常序列進行矯正，對缺陷基因精確地原位修復，不涉及基因組的其他任何改變。通過同源重組（homologous recombination）即基因打靶（gene targetting）技術將外源
關于基因治療的按基因操作介紹
一類為基因修正（gene correction）和基因置換（gene replacement），即將缺陷基因的異常序列進行矯正，對缺陷基因精確地原位修復，不涉及基因組的其他任何改變。通過同源重組（homologous recombination）即基因打靶（gene targetting）技術將
基因治療的操作分類
一類為基因修正（gene correction）和基因置換（gene replacement），即將缺陷基因的異常序列進行矯正，對缺陷基因精確地原位修復，不涉及基因組的其他任何改變。通過同源重組（homologous recombination）即基因打靶（gene targetting）技術將外源
基因治療按基因操作分類介紹
一類為基因修正（gene correction）和基因置換（gene replacement），即將缺陷基因的異常序列進行矯正，對缺陷基因精確地原位修復，不涉及基因組的其他任何改變。通過同源重組（homologous recombination）即基因打靶（gene targetting）技術將
Lissencephaly－gene－(LIS1)－in－neuronal－migration－and－development
Integration of pathways that regulate nucleokinesis during neuronal migration and a model of LIS1 mediating CLIP-170 interactions with the dynein/dyna
Gene－splicing－and－mutagenesis－by－PCRdriven－overlap－extension
實驗概要? ? ? ? Extension of overlapping gene segments by PCR is a simple, versatile technique for site-directed mutagenesis and gene splicing.Initial
基因表達輪廓（gene－expressed－profile）技術2
經過這輪ＲＤＡ過程,Tester中的目的DNA將得到第一次富集。將第一輪產物更換新接頭,進行第二輪RDA過程,目的DNA可得到進一步的富集。該技術假陽性很低。但仍不能解決個體mRNA在豐度上存在巨大差異的問題,當靶序列濃度較低時,其富集受抑制。3：抑制消減雜交（suppression subtrac
SUMOylation－as－a－mechanism－to－modulate－CtBPdependent－gene－responses
Covalent attachment of one eukaryotic protein to another is a prominent posttranslational modification and Ubiquitin is the most familiar of the prote