GeneStructureAnnotationatPlantGDB
The accurate identification of exons and introns that comprise a complete plant gene structure can be a time-consuming and challenging task. Novel Web-based tools facilitate the process by providing a convenient interface to current transcript evidence, and portals to relevant bioinformatics software. With a few keystrokes, the user can explore alternative transcript assemblies and, for example, select for ......閱讀全文
Gene－Structure－Annotation－at－PlantGDB
The accurate identification of exons and introns that comprise a complete plant gene structure can be a time-consuming and challenging task. Novel
Plant－Genome－Annotation－Methods
Annotation of plant genomic sequences can be separated into structural and functional annotation. Structural annotation is the foundation of all g
Structure－of－Mitochondria
The cytoplasm of nearly all eukaryotic cells contain mitochondria, although there is at least one exception, the protist?Chaos (Pelomyxa) carolinensis
A－Method－for－Structure5
ConclusionsQuorum-sensing signaling systems involving the interaction between a signaling peptide and its cognate histidine kinase receptor are widely
A－Method－for－Structure1
A Method for Structure–Activity Analysis of Quorum-Sensing Signaling Peptides from Naturally Transformable StreptococciMany species of streptococci se
A－Method－for－Structure4
We also used this mutant (SMdC) as a host to generate two types of?lacZ?transcriptional reporter fusion strains to assay the promoter activity of QS-c
A－Method－for－Structure2
CD SpectroscopyCD spectra for peptides and reference solutions were recorded at 298?K on a Jasco J-920 CD spectrometer with a 1-mm quartz cuvette. Spe
抗體結構（antibody－structure）
抗體（antibody，Ab）是機體免疫細胞被抗原激活后，由分化成熟的終末B細胞--漿細胞合成、分泌的一類能與相應抗原特異性結合的具有免疫功能的球蛋白。?抗體是具有4條多肽鏈的對稱結構，其中2條較長、相對分子量較大的相同的重鏈（H鏈），含450?550個氨基酸殘基，相對分子質量在 55000?7
A－Method－for－Structure3
CSP-Dependent Transformation AssayTo determine if synthetic peptides activated quorum sensing for induction of genetic competence, we used the?comC?de
The－TRC－shRNA－Design－Methods－and－Rules
OverviewWe design shRNA molecules with an algorithm. Our algorithm uses several criteria to rank potential 21mer targets within each human and mouse R
Mitochondria－Structure－and－Function－－Theoretical－Details
Mitochondria Structure and Function - Theoretical DetailsIn an attempt to be concise and understandable, introductory level courses and textbooks freq
The－TRC－shRNA設計方法與原則
OverviewWe design shRNA molecules with an algorithm. Our algorithm uses several criteria to rank potential 21mer targets within each human and mouse R
Use－of－the－GUS－Reporter－Gene
One of the most important considerations in the expression of heterologous proteins in plants is the choice of promoter. The study of promoter act
基因轉型(gene－transformation)
目的帶有特定基因的質體在分子生物研究上，是一個很重要的工具，將質體送入細菌的過程稱為基因轉形，經由基因轉型可使質體在細菌中大量復制，以備進一步研究。本實驗將把你在前面轉殖實驗中cDNA和質體DNA的連結反應送入細菌。 你將從本實驗學習如何進行基因轉型作用。原理早在1970年左右，有人發現細菌經由冰冷
Structure：損傷DNA末端降解過程機制
2022年7月15日，浙江大學生命科學學院趙燁教授、華躍進教授聯合浙江大學醫學院郭江濤教授團隊在CellPress旗下的Structure雜志發表了題為“Mechanisms of helicase activated DNA end resection in bacteria”的研究成果。該論文使
Reverse－Transfection－for－Gene－Function－Analysis
This guide describes a microarray-based system for the functional analysis in mammalian cells of many genes in parallel. Mammalian cells are cultured
FOSB－gene－expression－and－drug－abuse
Drug addiction is associated with long-term behavioral changes, suggesting a long-lived transcriptional regulator that responds to chronic drug exposu
Structure：貓咪幫助找尋抗HIV新藥物
如果你最近還沒有為你的貓咪做檢查，那你也可能不知道你的貓咪可能感染了艾滋病毒。貓免疫缺陷病毒肯能會引起貓咪致病，其癥狀類似感染HIV病毒后的癥狀。但是科學家認為這是研究抗HIV新藥的一種契機。 貓咪會怎么幫助人們抗擊艾滋病呢？不管是貓免疫缺陷病毒（FIV)還是人免疫缺陷病毒都會依賴一種稱為整合
Structure封面文章：解密白內障的形成
白內障是致盲的第一殺手，目前世界上有近兩千萬人受到它的影響。日前，加州大學Irvine分校的科學家們取得了重要成果，揭開了白內障的形成機制，這一成果將有望幫助人們為白內障患者恢復視力。這項研究作為封面文章刊登在十二月的Structure雜志上。 “我們的研究闡述了白內障形成的分子機制，這一
蛋白質結構預測(protein－structure－prediction)
一種生物體的基因組規定了所有構成該生物體的蛋白質，基因規定了組成蛋白質的氨基酸序列。雖然蛋白質由氨基酸的線性序列組成，但是，它們只有折疊成特定的空間構象才能具有相應的活性和相應的生物學功能。了解蛋白質的空間結構不僅有利于認識蛋白質的功能，也有利于認識蛋白質是如何執行其功能的。確定蛋白質的結構對于生物
差異基因表達研究方法介紹（DDPCR；GENEFISHING；GENE－CHIP）
差異基因表達的研究受到了廣泛的關注，常用的技術有DD-PCR；GENE-FISHING；GENE CHIP等。簡單介紹如下： DDRT -PCR技術即mRNA差異顯示聚合酶鏈式反應技術，此技術是以PCR技術和聚丙烯凝膠電泳技術為基礎，結合銀染或放射性自顯影等顯色技術，能快速有效地
Double－Stranded－RNA－Induced－Gene－Expression
One defense against viral infection is provided by PKR, double-stranded RNA activated protein kinase. When PKR interacts with dsRNA found in cells dur
Yeast－Gene－knockout－using－Oligo/PCR
Universal primers for gene knock-out using dominant drug markers: Kan, Clonat, and Hygromisin-B.?Forward primer: 5’ TCAGGGGCATGATGTGACT 3’Reverse prim
Control－of－Gene－Expression－by－Vitamin－D－Receptor
The vitamin D receptor, VDR is the mediator of all genomic actions of vitamin D3 and its analogs. It belongs to a family of ligand induced transcripti
PCR－Primers－For－Gene－Expression－Detection－or－Quantification
Why PrimerBank?PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time
報告基因(report－gene)的應用
報告基因被廣泛地應用于細胞生物學中的基因表達和細胞相關內容的研究。常用的報告基因主要有GUS基因、CAT基因、hGH基因、Cato2ase基因、綠色熒光蛋白基因及螢火蟲熒光素酶基因等。在這些報告基因中螢火蟲熒光素酶基因因其表達產物－熒光素酶敏感性高，測定方法快速且宜于掌握，線性好，并且熒光素酶產生的
Overview－of－telomerase－protein－component－gene－hTert－Transcriptional
Telomerase is an enzyme which replicates the terminal sequences of eukaryotic chromosomes, namely the telomeres. Cells which have an unlimited replica
Targeted－Gene－Replacement－in－Fungal－Pathogens－via－Agrobacterium－...
Genome sequence data on fungal pathogens provide the opportunity to carry out a reverse genetics approach to uncover gene function. Efficient meth
基因芯片(gene－chip)的原理
基因芯片(gene chip)的原型是80年代中期提出的。基因芯片的測序原理是雜交測序方法,即通過與一組已知序列的核酸探針雜交進行核酸序列測定的方法,在一塊基片表面固定了序列已知的八核苷酸的探針。當溶液中帶有熒光標記的核酸序列TATGCAATCTAG,與基因芯片上對應位置的核酸探針產生互補匹配時,通
GENEπ數字PCR技術應用教程
數字 PCR( digital PCR ，dPCR ) 下一代DNA/RNA擴增技術。原理是將一個PCR 反應體系分配到大量微小的反應單元中，在每個微反應器中包含或不包含 1 個或多個拷貝的目標核酸分子 (DNA 模板) ，進行“單分子模板”PCR 擴增。擴增結束后，通過陽性反應單元( 通過