UseoftheGUSReporterGene
One of the most important considerations in the expression of heterologous proteins in plants is the choice of promoter. The study of promoter activity is simplified in the majority of cases by the use of a readily detectable reporter gene. Indeed, reporter genes can also be used to aid the isolation of promoters, through the transformation of plants with a promoterless reporter gene construct. Activation o......閱讀全文
Use－of－the－GUS－Reporter－Gene
One of the most important considerations in the expression of heterologous proteins in plants is the choice of promoter. The study of promoter act
Leaf－GUS－Assay
一、實驗試劑 GUS Buffer (500 ml) 2.0478 g ? Na2HPO4 1.2688 g ? NaH2PO4 (=50 mM NaPi pH7.0) 10 ml ? ?0.5 M EDTA (=10 mM) 0.5 g ? ?Triton X-100 0.5 g ? ? N-L
Leaf－GUS－Assay
實驗概要a protocol for?Leaf GUS Assay?This protocol is for small samples (usually single leaf from 21DAI plants), scale up for larger samplesAs there are
Use－of－a－Hemacytometer
A hemacytometer (also spelled hemocytometer) is an etched glass chamber with raised sides that will hold a quartz coverslip exactly 0.1 mm above the c
Use－of－Micropipets
A. Gilson-style Pipetmen Micropipets (and similar models)We primarily use the Gilson micropipets in the core course labs. We have four sizes identifie
USE－誘變實驗
? ? ? ? ? ? 實驗材料 帶 mutS 表型（例如 BMH71-18) 的轉化用大腸桿菌感受態 帶 mut+表型的轉化用大腸桿菌感受態 試劑、試劑盒
USE－誘變實驗
本方法中，兩條寡核苷酸引物雜交到變性重組質粒 DNA 雙鏈的同一條鏈上。一條引物（誘變引物）攜帶一個擬引進靶 DNA 序列的突變，第二條引物攜帶一個能破壞質粒單一限制酶位點的突變。本實驗來源于分子克隆實驗指南（第三版）下冊，作者：〔美〕J. 薩姆布魯克 D.W. 拉塞爾。實驗材料帶 mutS 表型（
USE－誘變實驗
實驗材料 帶 mutS 表型（例如BMH71-18) 的轉化用大腸桿菌感受態帶 mut+表型的轉化用大腸桿菌感受態試劑、試劑盒 退火緩沖液貯存液合成緩沖液噬菌體 T4DNA 連接酶噬菌體 T4DNA 聚合酶或測序酶單一位點的限制性內切核酸酶瓊脂糖凝膠誘變引物選擇引物質粒 DNA儀器、耗材 70°C
USE－OF－THE－LIGHT－MICROSCOPE
USE OF THE LIGHT MICROSCOPEEach time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjus
Protein－Immunolocalization－in－Maize－Tissues
The ?analysis of gene expression at transcript and at protein level is of ?outstanding importance in plant developmental biology. Proteins can be ?loc
gus基因的檢測方法
根據gus基因檢測所用的底物不同，可以選擇三種檢測方法：組織化學法、分光光度法和熒光法（靈感度為分光光度檢測法最高）。其中最為常用的是組織化學法。組織化學法檢測以5-溴-4-氯-3-吲哚-β-葡萄糖苷酸（X-Gluc）作為反應底物，將被檢材料用含有底物的緩沖液浸泡。若組織細胞發生了gus基因的轉化，
Use－of－Transmission－Electron－Microscopy
?Use of Transmission Electron MicroscopyOverviewA protocol describing the use of Zeiss EM9-S transmission electron microscopy is presented.?MaterialZe
A－Yeast－Secretion－Trap－Assay－for－Identification－of－Secreted－Proteins－...
Secreted proteins from plants and phytopathogens play important roles in their interactions and contribute to elaborate mechanisms of attack, defe
Use－an－IgG－antiIgM...
實驗概要This method involves coupling an IgG anti-IgM antibody to protein A or G beads (see method below). These beads can then be used in the normal
Creation－and－Use－of－Infectious－Virus－Vector
Creation and use of your infectious vector:Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 ml of media. (This can be scaled up if desired).The f
CREATION－AND－USE－OF－YOUR－INFECTIOUS－VECTOR
實驗概要CREATION AND USE OF YOUR INFECTIOUS VECTOR實驗步驟Day 1? ? ? ? 1. Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 mL of media. (This can be scal
Gene－Structure－Annotation－at－PlantGDB
The accurate identification of exons and introns that comprise a complete plant gene structure can be a time-consuming and challenging task. Novel
基因轉型(gene－transformation)
目的帶有特定基因的質體在分子生物研究上，是一個很重要的工具，將質體送入細菌的過程稱為基因轉形，經由基因轉型可使質體在細菌中大量復制，以備進一步研究。本實驗將把你在前面轉殖實驗中cDNA和質體DNA的連結反應送入細菌。 你將從本實驗學習如何進行基因轉型作用。原理早在1970年左右，有人發現細菌經由冰冷
HOW－TO－USE－THE－COULTER－COUNTER－TO－COUNT－CELLS
1) Turn on the counter by pulling out the on/off button. You need to do this at least 10 min before use to obtain sufficient vacuum.Usually put 0.2 ml
Guidelines－for－the－Use－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal
What is Anesthesia??Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are?analgesia?(pain relief),?amn
登革熱基孔肯雅熱三合一核酸檢測試劑盒英文使用說明
Lot-No.Ref. FR342 Expiry time: 1 year100 Tests (Ready to use PCR kit) ??-Only for in vitro use--Only for research use –?To be used by a technical pers
賽默飛Ion－Torrent－Reporter軟件獲得CEIVD標志
賽默飛世爾科技近日表示，其 Ion Torrent Reporter 軟件非整倍體 Dx 工作流程已獲得 CE-IVD 標志。 CE-IVD 標志涵蓋兩個工作流程：低通全基因組非整倍體 Dx w1.0 和低通全基因組非整倍體 Mosaic Dx 1.0。這些工作流程將與一般的非體外診斷產品結合
生物分子學中GUS什么意思
應該是指的GUS基因，在植物樣品中很常用的一個報告基因。GUS基因就是β-葡萄糖苷酸酶基因 。該基因產物為β-葡萄糖苷酸酶，為一水解酶。可使用市售的底物 (substrate) 在原位 (in situ) 顯現出酶的活性，以肉眼即可檢測其產物，非常方便。
轉基因植物的GUS表達分析
實驗概要本實驗對轉基因擬南芥進行了GUS組織化學染色分析及GUS活性測定。主要試劑X-Gluc，丙酮，乙醇，BSA，考馬斯亮藍溶液主要設備顯微鏡(BX50 OLYPUS)，研缽，高速離心機，Nano drop核酸蛋白測定儀實驗材料轉基因擬南芥實驗步驟1. 轉基因植物的GUS組織化學染色分析?? 1)
病毒核酸檢測試劑盒英文使用說明
Lot-No.Ref. FR340 Expiry time: 1 year100 Tests (Ready to use PCR kit) ?Zika Virus ???(Real time) DOUBLE CHECK ????????????????????????????????????????
轉基因
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline?(University of Michigan Transgenic Animal Model Core)Thi
Reverse－Transfection－for－Gene－Function－Analysis
This guide describes a microarray-based system for the functional analysis in mammalian cells of many genes in parallel. Mammalian cells are cultured
FOSB－gene－expression－and－drug－abuse
Drug addiction is associated with long-term behavioral changes, suggesting a long-lived transcriptional regulator that responds to chronic drug exposu
Setup－and－use－of－a－twolaser－multiphoton－microscope
Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imagingDavid Entenberg,1 Jeffrey Wyckoff,1 Bojana Gligori
Basic－Theory－and－Use－of－GCMS（二）
For?ionisation?to?take?place?at?all,?chemical?reaction?between?the?sample?and?the?reagent?gas?must?be?exothermic.?The?grater?the?heat?of?the?reaction,