USEOFTHELIGHTMICROSCOPE
USE OF THE LIGHT MICROSCOPEEach time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjust the iris diaphragm and focus the condenser to give its optimum performance, this could result in objects in the preparation being undetected.There are many microscope set up procedures for specific functionality but the most versatile for general use is ‘Critical Illu......閱讀全文
USE－OF－THE－LIGHT－MICROSCOPE
USE OF THE LIGHT MICROSCOPEEach time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjus
Measurement－with－the－Light－Microscope
Measurement with the Light MicroscopeYour microscope may be equipped with a scale (called a reticule) that is built into one eyepiece. The reticule ca
Setup－and－use－of－a－twolaser－multiphoton－microscope
Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imagingDavid Entenberg,1 Jeffrey Wyckoff,1 Bojana Gligori
Use－of－SemiThin－Cryosections－for－Light－Microscopy.
Use of Semi-Thin Cryosections for Light Microscopy.Semi-thin sections can be obtained from frozen blocks of cryoprotected biological material by secti
Microscopes－in－Cell－Biology
Microscopes in Cell BiologyIntroductionMicroscopy has a major role in the study of cells. From the very beginning, researchers have tried to develop w
Light－Microscopy
The light microscope, so called because it employs visible light to detect small objects, is probably the most well-known and well-used research tool
顯微鏡技術——光學顯微技術
The Light Microscope?(House Ear Institute)An explanation of how the light microscope works, how to use it, and how to get optimal results when using i
Use－of－a－Hemacytometer
A hemacytometer (also spelled hemocytometer) is an etched glass chamber with raised sides that will hold a quartz coverslip exactly 0.1 mm above the c
Use－of－Micropipets
A. Gilson-style Pipetmen Micropipets (and similar models)We primarily use the Gilson micropipets in the core course labs. We have four sizes identifie
Transmission－Electron－Microscope－(TEM)
所謂TEM，就是一個放大鏡疊加了一臺照相機。這臺放大鏡的放大倍數比較高，可高達一百萬倍。當然，拋開分辨率談放大倍數都是耍流氓，那么，TEM的分辨率有多高呢？答案是 it depends。一般來說，TEM的分辨率要在1到2個納米，STEM更高，但是STEM得成像技術類似于SEM，但用的不是二次電子。我
Dark－Field－Viewing
Dark Field ViewingPrincipleTo view a specimen in dark field, an opaque disc is placed underneath the condenser lens, so that only light that is scatte
Phase－Contrast－Microscopy
Phase Contrast MicroscopyPrincipleMost of the detail of living cells is undetectable in bright field microscopy because there is too little contrast b
Live－imaging－with－Drosophila－tissue－culture－cells2
Materials & ReagentsDrosophila?Schneider S2 cellsSchneiders Medium (GIBCO/Invitrogen), 10% fetal calf serum, Antibiotics (Sigma A5955)Depression slide
USE－誘變實驗
? ? ? ? ? ? 實驗材料 帶 mutS 表型（例如 BMH71-18) 的轉化用大腸桿菌感受態 帶 mut+表型的轉化用大腸桿菌感受態 試劑、試劑盒
USE－誘變實驗
實驗材料 帶 mutS 表型（例如BMH71-18) 的轉化用大腸桿菌感受態帶 mut+表型的轉化用大腸桿菌感受態試劑、試劑盒 退火緩沖液貯存液合成緩沖液噬菌體 T4DNA 連接酶噬菌體 T4DNA 聚合酶或測序酶單一位點的限制性內切核酸酶瓊脂糖凝膠誘變引物選擇引物質粒 DNA儀器、耗材 70°C
USE－誘變實驗
本方法中，兩條寡核苷酸引物雜交到變性重組質粒 DNA 雙鏈的同一條鏈上。一條引物（誘變引物）攜帶一個擬引進靶 DNA 序列的突變，第二條引物攜帶一個能破壞質粒單一限制酶位點的突變。本實驗來源于分子克隆實驗指南（第三版）下冊，作者：〔美〕J. 薩姆布魯克 D.W. 拉塞爾。實驗材料帶 mutS 表型（
Protocol－to－Count－Cell－Number－of－Preimplantation－Embryos
Protocol to Count Cell Number of?Preimplantation?Embryos?using Nuclear Staining with Hoechst 33342 or DAPI??Introduction?The following is a simple pro
The－Effects－of－Ultraviolet－Light－on－the－Fertilization
Jill K. Flemming, Franklin & Marshall College, Class of 2001IntroductionThe objective of this project is to observe the effects of UV radiation on bot
Histochemistry－－Introduction
A few cell types are thin enough to be viewed directly in a microscope (algae, protozoa, blood, tissue cultures), but most tissues (kidney, liver, bra
Immunofluorecence－P...
實驗概要Immunofluorescence ?is a technique used for light microscopy with a fluorescence microscope ?and is used primarily on biological samples. This tec
Immunofluorescence－...
實驗概要Immunofluorescence ?is a technique used for light microscopy with a fluorescence microscope ?and is used primarily on biological samples. This tec
偏光顯微鏡(Polarizing－microscope)
一、偏光顯微鏡的特點偏光顯微是鑒定物質細微結構光學性質的一種顯微鏡。凡具有雙折射性的物質，在偏光顯微鏡下就能分辨的清楚，當然這些物質也可用染色法來進行觀察，但有些則不可能，而必須利用偏光顯微鏡。偏光顯微鏡的特點，就是將普通光改變為偏振光進行鏡檢的方法，以鑒別某一物質是單折射性（各向同性）或雙折射性（
Use－of－Transmission－Electron－Microscopy
?Use of Transmission Electron MicroscopyOverviewA protocol describing the use of Zeiss EM9-S transmission electron microscopy is presented.?MaterialZe
Use－of－the－GUS－Reporter－Gene
One of the most important considerations in the expression of heterologous proteins in plants is the choice of promoter. The study of promoter act
胚胎和成年斑馬魚眼情的組織學準備
INTRODUCTIONThis protocol describes the histological preparation of embryonic and adult zebrafish eyes. The methods described here can be easily adapt
Silver－Acetate－Autometallography－(AMG)
In the early eighties, a series of papers were published by Gorm DANSCHER, Aarhus, Denmark, to introduce a reliable and easy-to-handle technique for l
Silver－Enhancement－...
實驗概要The method provides a silver enhancement protocol for immunoassay.主要試劑Prepare the following reagents fresh daily except for the citrate buffer.1.
Immunofluorescence－/－Confocal－Microscopy－Protocol
實驗概要Immunofluorescence ?is a technique used for light microscopy with a fluorescence microscope ?and is used primarily on biological samples. This tec
Immunofluorescence－/－Confocal－Microscopy－Protocol
實驗概要Immunofluorescence ?is a technique used for light microscopy with a fluorescence microscope ?and is used primarily on biological samples. This tec
CREATION－AND－USE－OF－YOUR－INFECTIOUS－VECTOR
實驗概要CREATION AND USE OF YOUR INFECTIOUS VECTOR實驗步驟Day 1? ? ? ? 1. Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 mL of media. (This can be scal