UseofMicropipets
A. Gilson-style Pipetmen Micropipets (and similar models)We primarily use the Gilson micropipets in the core course labs. We have four sizes identified by the number on the round button on the plunger. The value is the maximum volume in microliters that can be transferred with that size pipet. Size indicator button on a Gilson P1000. Other autopipet sizes we have on hand. P1000 P200 P100 ......閱讀全文
Use－of－Micropipets
A. Gilson-style Pipetmen Micropipets (and similar models)We primarily use the Gilson micropipets in the core course labs. We have four sizes identifie
Use－of－a－Hemacytometer
A hemacytometer (also spelled hemocytometer) is an etched glass chamber with raised sides that will hold a quartz coverslip exactly 0.1 mm above the c
USE－誘變實驗
? ? ? ? ? ? 實驗材料 帶 mutS 表型（例如 BMH71-18) 的轉化用大腸桿菌感受態 帶 mut+表型的轉化用大腸桿菌感受態 試劑、試劑盒
USE－誘變實驗
本方法中，兩條寡核苷酸引物雜交到變性重組質粒 DNA 雙鏈的同一條鏈上。一條引物（誘變引物）攜帶一個擬引進靶 DNA 序列的突變，第二條引物攜帶一個能破壞質粒單一限制酶位點的突變。本實驗來源于分子克隆實驗指南（第三版）下冊，作者：〔美〕J. 薩姆布魯克 D.W. 拉塞爾。實驗材料帶 mutS 表型（
USE－誘變實驗
實驗材料 帶 mutS 表型（例如BMH71-18) 的轉化用大腸桿菌感受態帶 mut+表型的轉化用大腸桿菌感受態試劑、試劑盒 退火緩沖液貯存液合成緩沖液噬菌體 T4DNA 連接酶噬菌體 T4DNA 聚合酶或測序酶單一位點的限制性內切核酸酶瓊脂糖凝膠誘變引物選擇引物質粒 DNA儀器、耗材 70°C
USE－OF－THE－LIGHT－MICROSCOPE
USE OF THE LIGHT MICROSCOPEEach time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjus
A.－Gilsonstyle－Pipetmen－Micropipets－(and－similar－models)
We primarily use the Gilson micropipets in the core course labs. We have four sizes identified by the number on the round button on the plunger. The v
Use－of－Transmission－Electron－Microscopy
?Use of Transmission Electron MicroscopyOverviewA protocol describing the use of Zeiss EM9-S transmission electron microscopy is presented.?MaterialZe
Use－of－the－GUS－Reporter－Gene
One of the most important considerations in the expression of heterologous proteins in plants is the choice of promoter. The study of promoter act
Creation－and－Use－of－Infectious－Virus－Vector
Creation and use of your infectious vector:Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 ml of media. (This can be scaled up if desired).The f
Use－an－IgG－antiIgM...
實驗概要This method involves coupling an IgG anti-IgM antibody to protein A or G beads (see method below). These beads can then be used in the normal
CREATION－AND－USE－OF－YOUR－INFECTIOUS－VECTOR
實驗概要CREATION AND USE OF YOUR INFECTIOUS VECTOR實驗步驟Day 1? ? ? ? 1. Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 mL of media. (This can be scal
HOW－TO－USE－THE－COULTER－COUNTER－TO－COUNT－CELLS
1) Turn on the counter by pulling out the on/off button. You need to do this at least 10 min before use to obtain sufficient vacuum.Usually put 0.2 ml
Guidelines－for－the－Use－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal
What is Anesthesia??Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are?analgesia?(pain relief),?amn
Setup－and－use－of－a－twolaser－multiphoton－microscope
Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imagingDavid Entenberg,1 Jeffrey Wyckoff,1 Bojana Gligori
Basic－Theory－and－Use－of－GCMS（二）
For?ionisation?to?take?place?at?all,?chemical?reaction?between?the?sample?and?the?reagent?gas?must?be?exothermic.?The?grater?the?heat?of?the?reaction,
Use－of－the－Bradford－Protein－Assay－in－a－Microtiter－Plate－Format
Introduction?The Bradford protein assay is a simple procedure for determination of protein concentrations in solutions that depends upon the change in
Basic－Theory－and－Use－of－GCMS（一）
BASIC?THEORY?AND?USE?OF?GC-MSbyDr.?Eugenia?SobolevaContent1.??Introduction.2.??GC-MS?systems?and?components.3.??Vacuum?system3.1.??Rotary?pump3.2.??Di
General－Laboratory－Procedures,－Equipment－Use,－and－Safety－Considerations
A. Storage .The following properties of reagents and conditions are important considerations in processing and storing DNA and RNA. Heavy metals promo
Use－of－the－B.D.－FACS－or－Calibur－Flow－Cytometers
Reservations:Schedule time on a cytometer (FACS or Calibur) by filling in the calendar at the flow lab. Don't grossly overbook since we have to pa
How－to－use－Basic－Local－Alignment－Search－Tool－(BLAST)
DescriptionThe BLAST algorithm was developed as a way to perform DNA and?protein sequence similarity searches by an algorithm that is?faster than FAST
Use－of－SemiThin－Cryosections－for－Light－Microscopy.
Use of Semi-Thin Cryosections for Light Microscopy.Semi-thin sections can be obtained from frozen blocks of cryoprotected biological material by secti
Use－of－Nonaqueous－Fractionation－and－Metabolomics－to－Study－Chloroplast－...
Use of Non-aqueous Fractionation and Metabolomics to Study Chloroplast Function in ArabidopsisChloroplasts are the chemical factories of plant cells b
Isolation－of－Total－RNA－from－Animal－Cells－use－RNeasy－Mini－Kit
實驗概要Extract the total RNA from animal cells by using RNeasy Mini Kit (QIAGEN No.74104)?主要試劑SDS based extraction solution實驗步驟1. ?Harvest cells.1)?? Try
Use－of－the－B.D.－FACS－or－Calibur－Flow－Cytometers2
Gating:Gating will allow you to view cells of interest by any combination of criteria that you choose. Gating does not change the intensity value assi
病毒核酸檢測試劑盒英文使用說明
Lot-No.Ref. FR340 Expiry time: 1 year100 Tests (Ready to use PCR kit) ?Zika Virus ???(Real time) DOUBLE CHECK ????????????????????????????????????????
放射性同位素使用規則
RULES FOR THE USE OF RADIOACTIVITY?You must be certified by EHS before you can use radioactivity.??The guiding principle isCOMMON SENSE.??I take radio
MS－Plant－Tissue－Culture－Medium
Component mg/l in MS mg/l in stock Amount for
Pest－Control
We use Dimilin G (4% diflubenzuron) from Maag AG to fight the small dipteran black flies (Trauermücken). It is a granular substance and sprinkled
Tubulin－Basics
I. Useful Values1 mg/ml tubulin = 10 μM (assuming MW of ab-tubulin heterodimer is 100,000; in reality it is ~110,000 but almost all tubulin labs use t