PhageDNA
IntroductionThe phage lysate from the plate contains bacterial DNA and RNA, as well as phage DNA encased in the phage coat. The following procedure, digests bacterial RNA and DNA, precipitates the phage particles, then removes the phage coat with organic extraction. Finally, the DNA is purified by precipitation with alcohol and visualized by gel electrophoresis.Protocol 1. Add RNase A and DNase I, each to a fina......閱讀全文
Phage－DNA
IntroductionThe phage lysate from the plate contains bacterial DNA and RNA, as well as phage DNA encased in the phage coat. The following procedure, d
Lambda－Phage－DNA－Quickprep
suspend a single plaque in 1 ml PSBadsorb 10 min at 37°C: 0.1 ml eluted phage*/0.1 ml MgCa/0.1 ml saturated K802 culture grown in NZY broth/0.2% malto
Column－Method－for－Lambda－Phage－DNA－Preparation
Purpose: Mini-prep method for lambda phage DNA purification from lysates. Time required: 4 hours once the lysate is in hand Special suppl
Preparation－of－phage－particles－from－phage－vectors
Pick up one phage vectors-containing colony with a sterile loop and put into 10 ml? 2xTY + 10 μg/l tetracycline. Shake at 200 rpm and 37 °C unti
Phage－Titer
IntroductionLambda phage is a commonly used vector for transgenes. Its very high rate of infectivity makes it ideal for creating large numbers of clon
Preparation－of－Phage－Lysates
Preparation of Phage LysatesInoculate 5 ml of lambda-broth in a glass culture tube with a single colony of an appropriate host strain of?E. coli. Incu
HELPER－PHAGE－PREPARATION
HELPER PHAGE PREPARATION1. Grow an overnight of NM522 in NZCYM medium.2. Dilute overnight 1:100 and grow to an A600 = 0.3 (@2.5 x 108 cells/ml).3. Inf
烈性噬菌體（virulent－phage）和溫和噬菌體（temperate－phage）
噬菌體(bacteriaphage or phage)是病毒的一類，結構很簡單，基本上由一個蛋白質外殼包裹著一些核酸組成的。噬菌體的多樣性來自于組成其外殼的蛋白質的種類，以及其染色體的類型和結構的不同。（一）烈性噬菌體（ virulent phage）遺傳學上應用最廣泛的烈性噬菌體是大腸桿菌（ E.
Easy－Way－to－Clone－Genes－From－a－Phage－Library
Easy Way to Clone? The protocol is oriented towards a C. albicans genomic library I made in Lambda Zap II on 7/97. The overall sequence of ev
cDNA－AMPLIFICATION－FROM－LAMBDAPHAGE－LIBRARY
PREPARE SOLUTIONS1. SM buffer (1 L):Mix?5.8?g of NaCl,?2?g of MgSO4-7H2O,?50?mL of 1M Tris-HCl, pH 7.5,?0.5?mL of 2% gelatin, and dH2O to 1 L (Autocla
Standard－Operating－Procedures－for－T1Phage－Testing－Assay
I. Introduction:This assay uses a lawn of phage-susceptible?E. coli?(DH10B) embedded in a layer of agarose. This top agarose lays on a bed of standard
噬菌體的生長
Preparing Lawn Cells for M13 Cloning?(Life Technologies)Lawn cells require the F' episome for M13 infection and may be prepared??Streaking Lambda
Lambda噬菌體
·?????????Lambda DNA Preparation?(Stanford DNA Sequence & Technology Center)Detailed protocol for lambda DNA preparation with recipes·?????????Isolati
M13噬菌體
·?????????M13 Phage?(Michael Blaber)Very useful background information about M13: its infection, replication, packing, cloning. If you are new to phag
CDNA文庫
?CDNA文庫（主要內容如下）·?????????Construction of cDNA Library·?????????Construction of Genome DNA Library·?????????Library Screening??OthersConstruction of cD
Lambda（噬菌體）DNA－Miniprep
David HarryInstitute of Forest GeneticsUSDA Forest ServicePacific Southwest Research StationAugust 26, 1993Background :There are many published method
Preparing－Lambda－DNA
Preparing Lambda DNA1- Coliphage lambda DNA is a widely used vector for recombinant DNA. The middle third of its 48,000 bp contains no genes required
Genomic－Libraries
Genomic DNA libraries?Size of some genomes and chromosomes:Comparative Sequence Sizes(Bases)(yeast chromosome 3)350 ThousandEscherichia coli (bacteriu
核酸的修飾酶
The restriction/modification system in bacteria is a?small-scale immune systemfor protection from infection by foreign DNA.?W. Arber and S. Linn (1969
EZ－96?－M13－Isolation－Spin－Protocol
實驗概要 The ?E.Z.N.A.? family of products is an innovative system that radically ?simplifies the extraction and purification of nucleic acids from a ?v
Expression－Library－Screening－(Procaryotic)－Using－APFusion－Proteins
Outline:Bacteriophage lambda is a linear double stranded DNA, approximately 50 kB in size. The two sticky ends help the phage to recircularize after e
EZ－96?－M13－Isolation－Vacuum－Manifold－Protocol
實驗概要The ?E.Z.N.A.? family of products is an innovative system that radically ?simplifies the extraction and purification of nucleic acids from a ?vari
EZ－96?－M13－Isolation－Vacuum－Manifold－Protocol
實驗概要 The ?E.Z.N.A.? family of products is an innovative system that radically ?simplifies the extraction and purification of nucleic acids from a ?v
DNA克隆
DNA克隆（主要內容如下）·?????????General Procedure·?????????PCR Cloning·?????????Subcloning·?????????ET Cloning·?????????Vector Preparation·?????????Ligation Re
核酸以及核苷酸的基本換算
核酸以及核苷酸的基本換算1．核酸的換算：(1.1) 摩爾數與質量：1 mg 1,000bp DNA = 1.52 pmol1 mg pUC18/19 DNA (2,688bp) = 0.57 pmol1 mg pBR322 DNA (4,361bp) = 0.35 pmol1 mg SV40 DNA
什么是DNA噬菌體？
中文名稱DNA噬菌體英文名稱DNA phage定 義能感染細菌并在細菌內復制自身的DNA病毒。應用學科生物化學與分子生物學（一級學科），核酸與基因（二級學科）
細胞化學詞匯DNA噬菌體
中文名稱：DNA噬菌體英文名稱：DNA phage定 義：能感染細菌并在細菌內復制自身的DNA病毒。應用學科：生物化學與分子生物學（一級學科），核酸與基因（二級學科）
基因克隆(gene－clone)的幾種常用方法介紹2
其基本原理是：用一個在種源上相近的基因組將靶基因組中所有共同的基因掩蓋起來,而只暴露出特異的基因,在整個反應中只有特異基因能被擴增。其操作程序為:(1)用同一限制性內切酶(一般用Bam HI,Bgl II或Hind III)同時處理靶基因組和掩蓋基因組DNA，其中掩蓋基因組DNA的量至少要2
Single－Primer－(SemiRandom)－PCR
DescriptionSingle primer PCR allows amplification from known to unknown regions in chromosomes, phage, plasmids, large PCR products and other sources
λ－噬菌體末端酶的基本信息
中文名稱λ 噬菌體末端酶英文名稱λ phage terminase定 義λ噬菌體編碼的末端酶。能識別結合λDNA連環體中的cos序列，結合后特異性地切割DNA形成12個堿基的黏性末端。此酶還有識別蛋白質衣殼前體和ATP酶的功能，水解ATP提供能量。將切割后所形成的酶-DNA二元復合體運送到蛋白質衣