PhageTiter
IntroductionLambda phage is a commonly used vector for transgenes. Its very high rate of infectivity makes it ideal for creating large numbers of clones. Large numbers of clones are often needed for construction of gene libraries that represent large populations of molecules. These libraries are also easy to sort through, as lambda phage clones are particularly easy to array spatially at high density. This is called ......閱讀全文
Phage－Titer
IntroductionLambda phage is a commonly used vector for transgenes. Its very high rate of infectivity makes it ideal for creating large numbers of clon
Preparation－of－phage－particles－from－phage－vectors
Pick up one phage vectors-containing colony with a sterile loop and put into 10 ml? 2xTY + 10 μg/l tetracycline.Shake at 200 rpm and 37 °C untill the
Phage－DNA
IntroductionThe phage lysate from the plate contains bacterial DNA and RNA, as well as phage DNA encased in the phage coat. The following procedure, d
HELPER－PHAGE－PREPARATION
HELPER PHAGE PREPARATION1. Grow an overnight of NM522 in NZCYM medium.2. Dilute overnight 1:100 and grow to an A600 = 0.3 (@2.5 x 108 cells/ml).3. Inf
Preparation－of－Phage－Lysates
Preparation of Phage LysatesInoculate 5 ml of lambda-broth in a glass culture tube with a single colony of an appropriate host strain of?E. coli. Incu
烈性噬菌體（virulent－phage）和溫和噬菌體（temperate－phage）
噬菌體(bacteriaphage or phage)是病毒的一類，結構很簡單，基本上由一個蛋白質外殼包裹著一些核酸組成的。噬菌體的多樣性來自于組成其外殼的蛋白質的種類，以及其染色體的類型和結構的不同。（一）烈性噬菌體（ virulent phage）遺傳學上應用最廣泛的烈性噬菌體是大腸桿菌（ E.
Lambda－Phage－DNA－Quickprep
suspend a single plaque in 1 ml PSBadsorb 10 min at 37°C: 0.1 ml eluted phage*/0.1 ml MgCa/0.1 ml saturated K802 culture grown in NZY broth/0.2% malto
CDNA文庫
?CDNA文庫（主要內容如下）·?????????Construction of cDNA Library·?????????Construction of Genome DNA Library·?????????Library Screening??OthersConstruction of cD
Easy－Way－to－Clone－Genes－From－a－Phage－Library
Easy Way to Clone?The protocol is oriented towards a C. albicans genomic library I made in Lambda Zap II on 7/97.The overall sequence of events is:??
Expression－Library－Screening－(Procaryotic)－Using－APFusion－Proteins
Outline:Bacteriophage lambda is a linear double stranded DNA, approximately 50 kB in size. The two sticky ends help the phage to recircularize after e
Column－Method－for－Lambda－Phage－DNA－Preparation
Purpose:Mini-prep method for lambda phage DNA purification from lysates.Time required:4 hours once the lysate is in handSpecial supplies required:BioR
Preparation－of－High－Titer－Adenovirus－in－P11－cells
adapted from Cell Biology, A Lab Manual, second edition, volume 1?-Grow up P11 cells in 15 cm plates to 70 – 80% confluence.?-Infect cells with a MOI
cDNA－AMPLIFICATION－FROM－LAMBDAPHAGE－LIBRARY
PREPARE SOLUTIONS1. SM buffer (1 L):Mix?5.8?g of NaCl,?2?g of MgSO4-7H2O,?50?mL of 1M Tris-HCl, pH 7.5,?0.5?mL of 2% gelatin, and dH2O to 1 L (Autocla
Lambda（噬菌體）DNA－Miniprep
David HarryInstitute of Forest GeneticsUSDA Forest ServicePacific Southwest Research StationAugust 26, 1993Background :There are many published method
Standard－Operating－Procedures－for－T1Phage－Testing－Assay
I. Introduction:This assay uses a lawn of phage-susceptible?E. coli?(DH10B) embedded in a layer of agarose. This top agarose lays on a bed of standard
cDNA－LIBRARY－SCREENING
PREPARE SOLUTIONS1. 10mM MgSO4, 0.2% Maltose LB (100 mL):Mix?1.0?g of Bacto-Tryptone,?1.0?g of NaCl,?0.5?g of Yeast Extract, and?1.0?mL of 1M MgSO4. A
Cosmid－Cloning:－Cell－preparation,－DNA－packaging,－and－Cell－Transfection
Cosmid Cloning: Cell preparation, DNA packaging, and Cell TransfectionProtocol taken from Stratagene's Gigapack packaging extracts instruction man
最主流的細胞活力檢測方法—CTG(CELL－TITERGLO)發光法簡介
細胞增殖檢測技術已廣泛應用于分子生物學、遺傳學、腫瘤生物學、免疫學、藥理和藥代動力學等研究領域。細胞增殖是指細胞在周期調控因子的作用下，通過DNA復制、RNA轉錄和蛋白質合成等復雜反應而進行的分裂系列過程。細胞通過分裂的方式增殖，細胞增殖是生物體的重要生命特征 。單細胞生物以細胞分裂的方式產生新
最主流的細胞活力檢測方法——CTG(CELL－TITERGLO)發光法簡介
細胞增殖檢測技術已廣泛應用于分子生物學、遺傳學、腫瘤生物學、免疫學、藥理和藥代動力學等研究領域。細胞增殖是指細胞在周期調控因子的作用下，通過DNA復制、RNA轉錄和蛋白質合成等復雜反應而進行的分裂系列過程。細胞通過分裂的方式增殖，細胞增殖是生物體的重要生命特征 。單細胞生物以細胞分裂的方式產
Preparing－Lambda－DNA
Preparing Lambda DNA1- Coliphage lambda DNA is a widely used vector for recombinant DNA. The middle third of its 48,000 bp contains no genes required
熒光偏振法快速準確高通量檢測IgG和含Fc段衍生物
熒光偏振法快速、準確、高通量檢測IgG和含Fc段衍生物Dr. Carolanne DohertyValitacell, NIBRT, Fosters Avenue, Blackrock, Dublin, Ireland? ? BMG多功能酶標儀可應用于定量IgG的新技術Valita?TITER???
噬菌體的生長
Preparing Lawn Cells for M13 Cloning?(Life Technologies)Lawn cells require the F' episome for M13 infection and may be prepared??Streaking Lambda
Lambda噬菌體
·?????????Lambda DNA Preparation?(Stanford DNA Sequence & Technology Center)Detailed protocol for lambda DNA preparation with recipes·?????????Isolati
Cesium－Chloride－Purification－of－T7
SummaryCleaner stocks of T7 that concentrates and purifies T7 bacteriophage.ProtocolGrow 100mL of permissive cells to a density of 108?to 109?cells/ml
M13噬菌體
·?????????M13 Phage?(Michael Blaber)Very useful background information about M13: its infection, replication, packing, cloning. If you are new to phag
Studier－Lysate－Prep
SummaryHow to make a lysate from a plaque preparation. We also use this protocol for preparation of a quick stock from previously made lysate prep.Pro
溫和噬菌體的分類和特點介紹
根據噬菌體和宿主菌的關系，可將噬菌體分為兩類：一類噬菌體在宿主菌細胞內迅速增殖，產生許多子代噬菌體，并最終使宿主菌細胞破裂，這類噬菌體被稱為烈性噬菌體（ virulent phage）；另一類噬菌體感染宿主菌后不立即增殖，而是將其核酸整合（Integration）到宿主菌染色體中，隨宿主核酸的復制而
關于溫和噬菌體的基本介紹
根據噬菌體和宿主菌的關系，可將噬菌體分為兩類：一類噬菌體在宿主菌細胞內迅速增殖，產生許多子代噬菌體，并最終使宿主菌細胞破裂，這類噬菌體被稱為烈性噬菌體（ virulent phage）；另一類噬菌體感染宿主菌后不立即增殖，而是將其核酸整合（Integration）到宿主菌染色體中，隨宿主核酸的復
Screening－a－cDNA－Library
Screening a cDNA Libraryfor use with HybriZAP zebrafish cDNA librariesObjectivecDNA library screening allows detection of expressed genes for subseque
高效液相解決方案4大妙招破解抗體分析難題
妙招1：小身材，大智慧——進樣器帶餾分收集功能的雙三元 DGLC——U3000?1 離線方法在抗體開發前期，對樣品進行純化制備，進行親和色譜分析，將目標抗體餾分收集到進樣盤中，然后再進行后續的抗體藥的表征工作。?2 在線分析二維色譜PAT技術同時監控產品產量和質量，原本需要2天時間才知道產量和質量（