cDNA／AFLPProtocol
Preparation of Para-magnetic beads from Promega cat#Z5482:a) suspend magnetic particles in bottle - transfer 200 ul (200 ug) of beads per sampleof RNA to be purified to a microfuge tube or tubes.b) place microfuge tube in magnetic stand for 30 sec or longer to collect the beads.c) remove supernatent and wash 3X with 200 ul of 0.5X SSCd) finally resuspend the SA-PMPs in 100ul of 0.5X SSC for each 2......閱讀全文
TAIL－PCR－Protocol
TAIL is a series of reactions that are intended to map where a T-DNA (transfer DNA) has inserted within the genome. The main components of the 3 react
Microarray－Hybridization－Protocol
Introduction:One microarray set consists of 7 nylon membranes with 2.5 x 7.5 cm dimension. 2304 genes were spotted onto nylon membranes (Schleicher an
Nucleolar－Isolation－Protocol
We recommend that you first download and read this page as a?PDF file. Using that as your guide, you can then follow the protocol below and view a Qui
Intracellular－Staining－Protocol
1.?Fix cells- Add16% formaldehyde directly into culture medium to obtain a final concentration of 1.5% formaldehyde.2. Incubate in fixative for 10 min
Basic－ELISA－Protocol
實驗概要? ? ? ? There are many different types of ELISAs, which can detect the presence of protein in serum or supernatent. One of the most common typ
Western－Blotting－Protocol
實驗概要The western blot ?(sometimes called the protein immunoblot) is a widely used analytical ?technique used to detect specific proteins in the given s
Sandwich－ELISA－Protocol
實驗概要The ?Sandwich ELISA measures the amount of antigen between two layers of ?antibodies (i.e. capture and detection antibody). The antigen to be ?mea
RNA－Isolation－Protocol
RNA Isolation Protocol(Revised 5-15-2003)Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109?cells (OD600= 0.2 Dilute cells or scale up)
Immunofluorescence－Microscopy－Protocol
實驗概要Immunofluorescence ?allows the imaging of a specific factor in cells or tissue sections ?through the use of a specific antibody chemically which i
Phycoerythrin－conjugation－protocol
Phycoerythrin conjugation protocolDavid's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal an
Nuclear－Extraction－Protocol
實驗概要The procedure presented below describes a method for extracting nuclear from several cell lines of human origin.主要試劑Hypotonic Buffer Solution20 mM
Dot－blot－protocol
實驗概要A ?technique for detecting, analyzing, and identifying proteins, similar ?to the western blot technique but differing in that protein samples are
Cytotoxicity－Assays－Protocol
Cytotoxicity Assays ProtocolCell-mediated cytotoxicity was determined by using a standard microcytotoxicity assay. Briefly, target cells were pelleted
BrdU－Labeling－Protocol
實驗概要The thymidine analog, 5-bromo-2-deoxyuridine (BrdU),is a common reagent used for cell proliferation assays and for the detection of apoptotic
Streamlined－DNA－Extraction－Protocol
This method is derived from a procedure developed by Toby Bradshaw and the Poplar Molecular Genetics Cooperative. We have tested the procedure wit
Xenograft－Tumor－Assay－Protocol
1) Determine the number of cells for injection (ie 5′106 ) to determine the number of plates thatwill require trypsinizing (usually a 100% confluent p
Immunofluorescence－/－Confocal－Microscopy－Protocol
實驗概要Immunofluorescence ?is a technique used for light microscopy with a fluorescence microscope ?and is used primarily on biological samples. This tec
Protocol－for－Construction－of－BAC－Libraries
Protocol for Construction of BAC Libraries??????The bacterial artificial chromosome cloning (BAC) system is emerging as the system of choice for const
ELISA－Protocol－(General－Guidelines)
實驗概要Sandwich ?enzyme-linked immunosorbent assays (ELISAs) involve attachment of a ?capture antibody to a solid phase support. Samples containing known
stem－cell－culture－protocol
實驗概要stem cell culture protocol主要試劑cell culture supplies and reagentssEnvironment: cell culture requires a sterile environment, so it needs a separat
IHC－Protocol－for－Fr...
實驗概要The method provides a IHC protocol for free floating brain sections.實驗步驟1.?Coronal 30-40 μm sections cut on a freezing microtome. Sections colle
Native－chromatin－immunoprecipitation－protocol
實驗概要Native chromatin immunoprecipitation to query specific chromatin states of individual genes.?主要試劑10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl 30 mM
Xenograft－Tumor－Model－Protocol
Preparation of tumor cellsGrow cells in complete medium and exclude any contaminationWhen cells are 70-80% confluent, 3-4 hrs before harvesting, repla
Simplified－Arabidopsis－Transformation－Protocol
(Brief version for those who are familiar with the method)Steve Clough and Andrew Bent, University of Illinois at Urbana-Champaign.Our present proto
Immunohistochemistry－Protocol－for－Frozen－Sections
實驗概要The ?following is a general procedure guide for preparation and staining of ?acetone-fixed frozen tissues using a purified, unconjugated primary ?
The－protocol－for－LIC－by－Exonuclease－III
The protocol for LIC by Exonuclease III梁耀極1. Design the primers with 15-bp overlap;2. Digest the vector by proper restriction enzyme;For getting high
miPS建系protocol
實驗概要本實驗方法介紹了miPS建系的詳細操作流程。主要試劑所用試劑盒： 逆轉錄病毒體系試劑盒? MIPD0-000-001慢病毒體系試劑盒????? MIPD-000-02規格：5次???????????組分名稱貨號規格數量逆轉錄病毒體系/慢病毒體系20ul/支5支助轉因子10ul1支小鼠iPS細
Marcantonio－Lab－Protocol－Manual
Protein GelPreparing and Running a Protein Gel (7% Polyacrylamide)?A. Preparation of Running Gel Solution?1) Add to a 50 ml cylinder:DD-H2O 26 ml30% a
T－cell－Activation－Protocol
IntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequ
Native－chromatin－immunoprecipitation－protocol
實驗概要The method is a native chromatin immunoprecipitation protocol.主要試劑1. 10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl 30 mM CaCl2 20 mM MgCl2 50 mM Na