cDNA／AFLPProtocol
Preparation of Para-magnetic beads from Promega cat#Z5482:a) suspend magnetic particles in bottle - transfer 200 ul (200 ug) of beads per sampleof RNA to be purified to a microfuge tube or tubes.b) place microfuge tube in magnetic stand for 30 sec or longer to collect the beads.c) remove supernatent and wash 3X with 200 ul of 0.5X SSCd) finally resuspend the SA-PMPs in 100ul of 0.5X SSC for each 2......閱讀全文
RLGS－protocol
A. Preparation of DNA SolutionIn the case of rice, for example This method may be appllicable for many grass species and some other plants.????????
ELISA－protocol
ELISA protocol：1.取5－10ul BMMY表達上清用0.05M NaHCO3稀釋到100ul鋪ELISA板，37度或室溫振蕩大于1小時。注意一定要做一個GS115空菌株表達上清作為陰性對照，最好還找一個帶有histag的蛋白作為陽性對照。2.TPBS洗板3次，方法：倒掉鋪板液，倒置于
NAi－protocol
siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western
Immunoprecipitation－Protocol
實驗概要Immunoprecipitation ?is a procedure by which proteins or peptides that react specifically ?with an antibody are removed from solution and examined
PCR－protocol
PCR reactionProtocol for 50μl reaction - adjust amounts if necessary, for a 20μl reaction use the same volumes of primer and dNTP-mix, but adjust the
RNAi－protocol
?siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western
ELISPOT－Protocol
實驗概要The ?Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method ?of measuring the antibody or cytokine production of immune cells on t
Immunoblot－Protocol
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
ELISPOT－protocol
實驗概要The procedure ?below is a general guideline procedure for ELISPOT. Abcam ELISPOT kits ?have been designed for detection of various cytokines and g
ELISPOT－Protocol
實驗概要The ?Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method ?of measuring the antibody or cytokine production of immune cells on t
Migration－Assay－Protocol
Materials to be prepared beforehand:1) FBS free medium2) 10% FBS medium3) Cell migration filter insert ( Transwell?, 12mm Diameter, 12 μm Pore Size.)P
cDNA/AFLP－Protocol
Preparation of Para-magnetic beads from Promega cat#Z5482:a) suspend magnetic particles in bottle - transfer 200 ul (200 ug) of beads per sampleof RNA
Urea－Lysis－Protocol
Urea?lysis?buffer????????????9M Urea, 2.5mM EDTA, 2.5mM EGTA, 1% DTE, 4% CHAPS????????????make 10ml and aliquot 10x1ml, freeze at -70°C?Lysate?prepara
Dot－Blot－Protocol
a. Label nitrocellulose membrane (using a pencil) to identify protein elution fractions.b . Pipette 2μl from each fraction onto the membrane, allow th
TAIL－PCR－Protocol
TAIL is a series of reactions that are intended to map where a T-DNA (transfer DNA) has inserted within the genome. The main components of the 3 react
Intracellular－Staining－Protocol
1.?Fix cells- Add16% formaldehyde directly into culture medium to obtain a final concentration of 1.5% formaldehyde.2. Incubate in fixative for 10 min
Cell－Extraction－Protocol
實驗概要Primary tissues ?are valuable tools for the study of intracellular and extracellular ?markers which characterize disease states. We have developed
Colony－PCR－Protocol
1. Pull out eight glycerol stock plates from the –80oC freezer and set on bench top to thaw. Be sure to remove the foil seal before leaving the plates
Transformation－Protocol－for－Arabidopsis
Transformation Protocol for Arabidopsis – AbbreviatedGerminate seed in pots↓ 4 weeksStreak bacteria onto YM/MinA↓ 2-3 days 28°CSpray/dip bacterial sus
Nuclear－Extraction－Protocol
實驗概要The procedure presented below describes a method for extracting nuclear from several cell lines of human origin.主要試劑Hypotonic Buffer Solution20 mM
Protocol－for－dsRNA－Synthesis
實驗概要? ? ? ? We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends
Sandwich－ELISA－Protocol
實驗概要The ?Sandwich ELISA measures the amount of antigen between two layers of ?antibodies (i.e. capture and detection antibody). The antigen to be ?mea
Cytotoxicity－Assays－Protocol
Cytotoxicity Assays ProtocolCell-mediated cytotoxicity was determined by using a standard microcytotoxicity assay. Briefly, target cells were pelleted
Tissue－Harvest－Protocol
TISSUES TO BE PROCURED(minimally and preferably within 5-8 hours after death):1. Brain2. Liver3. Muscle4. Skin5: Others as the specific case dictatesP
Dot－blot－protocol
實驗概要A ?technique for detecting, analyzing, and identifying proteins, similar ?to the western blot technique but differing in that protein samples are
Protocol－of－Northern－blot
Protocol of Northern blot質粒的轉化和擴增質粒的鑒定目的基因片段的切割3.1樣品雙酶切(175μl水解體系)DW 115μlBuffer B(10×) 17.5μlBaM H 15μlPst I 17μlDNA(MMP-9) 16μlBSA 4.5μl37℃水浴，3h。3.2
Nucleolar－Isolation－Protocol
We recommend that you first download and read this page as a?PDF file. Using that as your guide, you can then follow the protocol below and view a Qui
Western－Blotting－Protocol
實驗概要The western blot ?(sometimes called the protein immunoblot) is a widely used analytical ?technique used to detect specific proteins in the given s
Yale－Immunofluorescence－Protocol
實驗概要We provide a protocol for fixation, immunostaining, and imaging in 384-well Plates.主要試劑Reagents1.?384-well view plates (Aurora)2.?HUVEC (pooled, L
Immunofluorescence－Microscopy－Protocol
實驗概要Immunofluorescence ?allows the imaging of a specific factor in cells or tissue sections ?through the use of a specific antibody chemically which i