TheprotocolforLICbyExonucleaseIII
The protocol for LIC by Exonuclease III梁耀極1. Design the primers with 15-bp overlap;2. Digest the vector by proper restriction enzyme;For getting high quality vectors, there are some good advices as follows:1) The restriction sites we choose should be 5’-overhangs orblunt ends , but it shouldn’t be 3'-protruding ends ;2) Digestion by double enzymes;3) Digestion the vector overnight to make sure complete cleavage a......閱讀全文
The－protocol－for－LIC－by－Exonuclease－III
The protocol for LIC by Exonuclease III梁耀極1. Design the primers with 15-bp overlap;2. Digest the vector by proper restriction enzyme;For getting high
足跡法(Footprinting)
Footprinting Procedures·?????????DNase I Footprinting?(Mike A. Dyer)·?????????DNase I footprintingDetermining the site of binding for a protein on a D
DNA測序
DNA測序（主要內容如下）·?????????Sequencing Gel Preparation·?????????Preparation of Templates?·?????????DNA Sequencing by the Dideoxy Method·?????????DNA Sequen
關于鋰電池的化學反應式介紹
1、鋰鈷氧化物電池 在陽極處，鋰被氧化。鋰離子與電子一起從碳中釋放出來： LiC6→xLi++xe?+C6LiC6→xLi++xe?+C6 在陰極處，鋰離子被二氧化鋰吸收，電極被還原，因為它也從電路接收電子： Li1?xCoO2+xLi++xe?→LiCoO2Li1?xCoO2+xLi+
NAi－protocol
siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western
ELISA－protocol
ELISA protocol：1.取5－10ul BMMY表達上清用0.05M NaHCO3稀釋到100ul鋪ELISA板，37度或室溫振蕩大于1小時。注意一定要做一個GS115空菌株表達上清作為陰性對照，最好還找一個帶有histag的蛋白作為陽性對照。2.TPBS洗板3次，方法：倒掉鋪板液，倒置于
RLGS－protocol
A. Preparation of DNA SolutionIn the case of rice, for example This method may be appllicable for many grass species and some other plants.????????
ELISPOT－Protocol
實驗概要The ?Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method ?of measuring the antibody or cytokine production of immune cells on t
ELISPOT－Protocol
實驗概要The ?Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method ?of measuring the antibody or cytokine production of immune cells on t
Immunoblot－Protocol
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
ELISPOT－protocol
實驗概要The procedure ?below is a general guideline procedure for ELISPOT. Abcam ELISPOT kits ?have been designed for detection of various cytokines and g
PCR－protocol
PCR reactionProtocol for 50μl reaction - adjust amounts if necessary, for a 20μl reaction use the same volumes of primer and dNTP-mix, but adjust the
Immunoprecipitation－Protocol
實驗概要Immunoprecipitation ?is a procedure by which proteins or peptides that react specifically ?with an antibody are removed from solution and examined
RNAi－protocol
?siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western
RNA－FISH－on－cultured－cells－in－interphase
IntroductionFluorescence?in situ?hybridization (FISH) has become a widely used method in genome and molecular genetic studies. The technique is highly
DNA的誘變和甲基化
·?????????In Vitro Mutagenesis Using Altered Sites?(Bowtell Lab)?In vitro Mutagenesis with dut ung single stranded DNA?(Hahn Lab)·?????????Site-direct
Phycoerythrin－conjugation－protocol
Phycoerythrin conjugation protocolDavid's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal an
RNA－Isolation－Protocol
Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109 cells (OD600= 0.2 Dilute cells or scale up)Pipet 30 ml of RNAProtect Bacteria Reagen
Yale－Immunofluorescence－Protocol
實驗概要We provide a protocol for fixation, immunostaining, and imaging in 384-well Plates.主要試劑Reagents1.?384-well view plates (Aurora)2.?HUVEC (pooled, L
cDNA/AFLP－Protocol
Preparation of Para-magnetic beads from Promega cat#Z5482:a) suspend magnetic particles in bottle - transfer 200 ul (200 ug) of beads per sampleof RNA
Silver－Staining－Protocol
1x 40min - overnight?????50% MeOH, 12% Acetic Acid1x 30min??????????????????????????????????50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde3x 20min?
Immunofluorescence－Microscopy－Protocol
實驗概要Immunofluorescence ?allows the imaging of a specific factor in cells or tissue sections ?through the use of a specific antibody chemically which i
Intracellular－Staining－Protocol
1.?Fix cells- Add16% formaldehyde directly into culture medium to obtain a final concentration of 1.5% formaldehyde.2. Incubate in fixative for 10 min
Cell－Extraction－Protocol
實驗概要Primary tissues ?are valuable tools for the study of intracellular and extracellular ?markers which characterize disease states. We have developed
Cytotoxicity－Assays－Protocol
Cytotoxicity Assays ProtocolCell-mediated cytotoxicity was determined by using a standard microcytotoxicity assay. Briefly, target cells were pelleted
Nuclear－Extraction－Protocol
實驗概要The procedure presented below describes a method for extracting nuclear from several cell lines of human origin.主要試劑Hypotonic Buffer Solution20 mM
TAIL－PCR－Protocol
TAIL is a series of reactions that are intended to map where a T-DNA (transfer DNA) has inserted within the genome. The main components of the 3 react
Migration－Assay－Protocol
Materials to be prepared beforehand:1) FBS free medium2) 10% FBS medium3) Cell migration filter insert ( Transwell?, 12mm Diameter, 12 μm Pore Size.)P
Protocol－for－Trichl...
實驗概要The ?efficiency of nucleotide incorporation in DNA/RNA polymerization ?reactions (e.g. transcription, reverse transcription, and DNA ?replication)
Bacteria－Culture－Protocol
Bacteria Culture ProtocolBy 徐曉政1、TBS Medium Preparation:Prepare 1L of TBS medium contains:Tryptone 12gYeast extract 24gNaCl 5gSodium Succinate 5gGlyce