RegulativeDevelopmentinAxolotlEmbryos;SplittingtheHeartField
ObjectiveThis experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically, we will split the morphogenetic field that is responsible for heart formation using grafted tissue from the gill area, and attempt to form two hearts – one on either side of the grafted tissue.BackgroundOrganisms that use regulative development rely on cell-cell interactions to determine wh......閱讀全文
Regulative－Development－in－Axolotl－Embryos;－Splitting－the－Heart－Field
ObjectiveThis experiment will seek to demonstrate regulative development in?Ambystoma mexicanum?embryos. Specifically, we will split the morphogenetic
Development－of－the－Heart－Morphogenetic－Field－in－the－Axolotl－Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
Axolotl－Protocol:－Transplantation－of－the－Gill－Organ－Field
Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ fie
Developmental－and－phenotypic－implications－of－transplantation
ObjectiveIn this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate the
Axolotl－Microsurgery
I.?Embryo Preparation1) Manually dejelly embryo of appropriate stage. Use fine foreps to break hole in hard jelly coat. Remove membrane around embryo
NFAT－and－Hypertrophy－of－the－heart－(Transcription－in－the－broken－heart)
Hypertrophy associated with both hypertension and obstruction to ventricular outflow leads to pathologic cardiac growth and it is associated with incr
Routine－Splitting－and－freezing－of－cells
1. Grow cells to subconfluence in a flask.2. Harvest as per normal and count.3. Spin down 5min 1.2K in benchtop. Resuspend at 1.0 X 106/ml in 10% DMSO
Pulse－Field－Electrophoresis
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fra
Dark－Field－Viewing
Dark Field ViewingPrincipleTo view a specimen in dark field, an opaque disc is placed underneath the condenser lens, so that only light that is scatte
In－VitroCulture－of－Chicken－Heart
The chicken is a classic organism used to illustrate the principles of basic embryology. One of the developmental systems which has been examined in g
Fate－Mapping－Axolotl－Embryos－in－Early－Gastrulation
BackgroundSince different amphibians execute gastrulation in different ways, the study of amphibian gastrulation has been complex. A popular method us
Actions－of－Nitric－Oxide－in－the－Heart
Nitric oxide (NO) has a number of important physiological actions in the cardiovascular system. In the heart, NO plays role in keeping the vessels pat
Development－(Immuno...
實驗概要Peprotech provides a development (immunostaining) protocol.實驗步驟1.?After the transfer is complete, incubate the membrane in blocking solution (3% N
Investigating－the－effect－ofthe－teratogen－cyclopamine－on－Ambystoma－mexicanum
ObjectiveThis experiment attempted to determine the effects of the teratogen cyclopamine on the Shh signaling pathway in axolotl embryos. Groups of ax
Hop－Pathway－in－Cardiac－Development
Homeodomain transcription factors comprise a large family of DNA binding factors that regulate transcription and development. Many homeodomain genes a
Solutions－for－Sea－Urchin－Development
Solutions for Sea UrchinASW: Artificial sea water (MBL)NaCl24.72 gKCl0.67 gCaCl2.2H2O1.36 gMgCl2.6H2O4.66 gMgSO4.7H2O6.29 gNaHCO30.18 gdH2Oto 1 literS
Melanocyte－Development－and－Pigmentation－Pathway
Studies with homozygous knockout mice have suggested that stem cell factor (SCF or KITLG), c-Kit, microphthalmia (Mitf), and B-cell CLL/lymphoma 2 (Bc
The－Effects－of－Ethanol－on－Zebrafish－Development
Introduction:The early stages of zebrafish,?Danio rerio?development are characterized by meroblastic dicoidal cleavage. Initially cell division only o
曠場實驗－(open－field－test)
實驗概要 觀察實驗動物在新異環境中的自主行為、探究行為與緊張度。實驗原理 曠場（open field test）分析系統是觀察研究實驗動物神經精神變化、進入開闊環境后的各種行為，例如動物對新開闊環境的恐懼而主要在周邊區域活動，在中央區域活動較少，但動物的探究特性又促使其產生在中央區域活動的動機，也可
曠場實驗－(open－field－test)
實驗概要觀察實驗動物在新異環境中的自主行為、探究行為與緊張度。實驗原理曠場（open field test）分析系統是觀察研究實驗動物神經精神變化、進入開闊環境后的各種行為，例如動物對新開闊環境的恐懼而主要在周邊區域活動，在中央區域活動較少，但動物的探究特性又促使其產生在中央區域活動的動機，也可
Effect－of－ethanol－on－development－of－Danio－reriro
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
開場實驗/敞箱實驗（Open－field－test）
研究發現，動物在敞箱中，由于對于新異環境的恐懼，主要在敞箱內的周邊部活動，而在中央區活動較少，但是動物的探究本性有必然促使它試圖在敞箱的中央區活動（包括活動、飲水或進食），從而產生沖突行為。抗焦慮藥物在不改變動物一般運動的情況下可以增加實驗動物進入敞箱中中央區的次數和中央區停留時間，或者增加實驗動物
安捷倫授予Jennifer－Field教授“思想領袖獎”
2023年11月16日，北京——安捷倫科技公司（紐約證交所：A）近日宣布授予Jennifer Field博士“安捷倫思想領袖獎”，獎項旨在表彰其在有機微量污染物和PFAS領域的開創性工作。Jennifer Field博士現任俄勒岡州立大學農學院環境和分子毒理學系的教授。她專注于對天然和改造系統中
The－effect－of－temperature－on－the－embryonic－development－of－sea－urchins
Objective:?This experiment aims to discover if temperature has an effect on the rates of sea urchin embryonic development. By altering the external en
Human－Embryonic－Stem－(ES)－Cell－Protocols——Splitting－Human－ES－cells－on－MEFs
based on splitting onto one plateWarm collagenase IV split media to 37 °C in a water bath.Aspirate media off of cell culture plate.Add the following a
Human－Embryonic－Stem－(ES)－Cell－Protocols—Splitting－Human－ES－cells－onMatrige
based on splitting onto on plateWarm collagenase media to 37°C in a water bath.Aspirate media off of cell culture plate.Add the following amount of co
Angiotensinconverting－enzyme－2－regulates－heart－function
Cardiovascular diseases are predicted to be the most common cause of death worldwide by 2020. The major regulator of blood pressure homeostasis is the
Developmental－Effects－of－Transplantation－of－Cell
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mos
The－Developmental－Effects－of－Nicotine－on－Chick－Embryos
More than 22 million American women smoke and approximately 25% of these women continue to smoke during pregnancy (Lambers and Clark 1996). Cigarette
ALK－in－cardiac－myocytes
Heart formation is cued by a combination of positive and negative signals from surrounding tissues. Inhibitory signals that block heart formation in a