DevelopmentoftheHeartMorphogeneticFieldintheAxolotlEmbryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative development at some point during their growth. Regulative development generally occurs in early gastrulation when cells are induced to form different structures according to the cell-cell signaling interactions in a specific area of the embryo that lead to the conditiona......閱讀全文
Development－of－the－Heart－Morphogenetic－Field－in－the－Axolotl－Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
Regulative－Development－in－Axolotl－Embryos;－Splitting－the－Heart－Field
ObjectiveThis experiment will seek to demonstrate regulative development in?Ambystoma mexicanum?embryos. Specifically, we will split the morphogenetic
Axolotl－Protocol:－Transplantation－of－the－Gill－Organ－Field
Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ fie
Developmental－and－phenotypic－implications－of－transplantation
ObjectiveIn this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate the
Fate－Mapping－Axolotl－Embryos－in－Early－Gastrulation
BackgroundSince different amphibians execute gastrulation in different ways, the study of amphibian gastrulation has been complex. A popular method us
Developmental－Effects－of－Transplantation－of－Cell
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mos
Investigating－the－effect－ofthe－teratogen－cyclopamine－on－Ambystoma－mexicanum
ObjectiveThis experiment attempted to determine the effects of the teratogen cyclopamine on the Shh signaling pathway in axolotl embryos. Groups of ax
In－VitroCulture－of－Chicken－Heart
The chicken is a classic organism used to illustrate the principles of basic embryology. One of the developmental systems which has been examined in g
The－Developmental－Effects－of－Nicotine－on－Chick－Embryos
More than 22 million American women smoke and approximately 25% of these women continue to smoke during pregnancy (Lambers and Clark 1996). Cigarette
Observation－of－living－and－plasticembedded－chick－embryos
The development of chick embryos has been studied since Aristotle. It is one of the most intensely studied organisms. One reason for this is that ther
The－effects－ofdifferent－concentrations－oflithium－chloride－on－thedevelopment
AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular?Brachydanio rerio?embryos results in
Axolotl－Microsurgery
I.?Embryo Preparation1) Manually dejelly embryo of appropriate stage. Use fine foreps to break hole in hard jelly coat. Remove membrane around embryo
NFAT－and－Hypertrophy－of－the－heart－(Transcription－in－the－broken－heart)
Hypertrophy associated with both hypertension and obstruction to ventricular outflow leads to pathologic cardiac growth and it is associated with incr
The－Effects－of－CalciumFree－Seawater－onthe－Development－ofSea－Urchin－Embryos
ObjectiveThe purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were pl
ALK－in－cardiac－myocytes
Heart formation is cued by a combination of positive and negative signals from surrounding tissues. Inhibitory signals that block heart formation in a
Embryo－Lysates－－Immunoprecipitation
Embryo lysatesTake 25 embryos and place into 1.7ml centrifuge tube.Rinse once in lysis buffer (add ~ 1ml) and remove by aspirationAdd 500 μL lysis buf
Chick－Embryo－staging
In order to repeat a published experiment, or have someone else repeat yours, it is important to use the same materials. For developmental studies, th
Pulse－Field－Electrophoresis
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fra
Dark－Field－Viewing
Dark Field ViewingPrincipleTo view a specimen in dark field, an opaque disc is placed underneath the condenser lens, so that only light that is scatte
MEDIA－FOR－EMBRYO－CULTURE－AND－MANIPULATION
M16 Medium (Protocol obtained from Karen Austen-Reed from SS Tan Laboratory, Anatomy Department)For oocyte maturation and routine culture of mouse emb
Embryo－Dissection－and－Micromanipulation－Tools
Embryo Dissection and Micromanipulation ToolsHazel L. Sive,?Robert M. Grainger, and?Richard M. HarlandAdapted from "Equipment for Embryo Experiments,"
Immature－Embryo－Rescue－and－Culture
Embryo culture techniques have many significant applications in plant breeding, as well as basic studies in physiology and biochemistry. Immature
DEVELOPMENTAL－EFFECTS－OF－LITHIUM－CHLORIDE－ON－XENOPUS－EMBRYOS
PURPOSE: The objective of this experiment is to study the effect of lithium chloride treatment on early blastula?xenopus?embryos.INTRODUCTION: When th
Actions－of－Nitric－Oxide－in－the－Heart
Nitric oxide (NO) has a number of important physiological actions in the cardiovascular system. In the heart, NO plays role in keeping the vessels pat
Effect－of－ethanol－on－development－of－Danio－reriro
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
ISOLATION－OF－PRIMARY－MOUSE－EMBRYO－FIBROBLASTS
You will need:13.5 day pregnant mouse (we use MTK NEO inbred white mice)2 sets sterile instrumentsone containing a pair of curved forceps and a pair o
MEDIUM－FOR－EMBRYO－RESCUE－(FOR－1－L)
Put 500 mL of distilled water in a 1-L beaker.Add the following:ucrose (6 % final concentration) – 30 gi-inositol (100 mg/L stock is 0.5 g/50 mL) – 10
Development－(Immuno...
實驗概要Peprotech provides a development (immunostaining) protocol.實驗步驟1.?After the transfer is complete, incubate the membrane in blocking solution (3% N
Determining－the－effects－of－nicotine－on－bone－－in－chicks
ObjectiveThe purpose of this experiment is to determine how the development of bone and cartilage in a chick are affected by treating the embryo with
The－Effect－of－Caffeine－on－the－Heart－Rates－of－ThreeDay－Old－Chick－Embryos
Objective??????The purpose of this experiment is to observe the effect of caffeine on the heart rate of three day old chick embryos by directly treati