SmallScaleYeastWholeCellExtractforIP
Grow 100 ml yeast cells in desired media overnight to an A600 of ~1.0 (0.6 to 1.2 works well). For growth in minimal media, 1 ml of a saturated overnight culture in minimal media (Synthetic dextrose (SD) with only the required amino acids) was inoculated to 100 ml of the same media and grown ~16 hrs at 30 degrees.Harvest cells and wash with 20 ml of cold extraction buffer in a 50 ml tube.Resuspend cell......閱讀全文
Small－Scale－Yeast－Whole－Cell－Extract－for－IP
Grow 100 ml yeast cells in desired media overnight to an A600 of ~1.0 (0.6 to 1.2 works well).?For growth in minimal media, 1 ml of a saturated overni
酵母準備
Yeast DNA PreparationYeast Genomic Preparation? (Gottschling Lab)Rapid method for yeast genomic DNA isolation??Yeast DNA Preparation (rapid glass bead
細胞核提取
HeLa Cell Nuclei Preparation?(John Garland)Prepare nuclear extract from HeLa cell??·?????????Extract Preparation?(Brent Graveley)Preparation of nuclea
Small－scale－DNA－preps－for－Neurospora－crassa.
Molecular biology experiments often require preparation of small amounts of DNA from many samples. This abbreviated DNA isolation method yields an ave
Direct－PCR－from－Whole－Yeast－Cells:－Zymolyase－Method
Direct PCR from Whole Yeast Cells: Zymolyase MethodContributor: Namjin ChungDate: June 18, 19961. An average-size yeast colony (0.5-2mm) or a cell pel
SDS－Whole－Cell－Extracts
-Use sterile technique and sterile solutions in steps 1 to 3.-1. Using a saturated starter culture, inoculate 25 to 30 ml of appropriate media in a 12
Live－Cell－Imaging－of－Yeast
Live Cell Imaging of YeastDaniel R. Rines, Dominik Thomann, Jonas F. Dorn, Paul Goodwin and Peter K. SorgerINTRODUCTIONThe development of cloning vect
酵母轉化
·?????????Yeast Transformation?(Gietz Lab)LiAc/SS-DNA/PEG Transformation·?????????Yeast Transformation?(Breeden Lab)LiAc method·?????????Large-Scale Y
Yeast－Cell－Cycle－by－Flow－Cytometry
ReagentsCold absolute ethanol.0.5 M Na citrate stock (filtered), 50mM diluted stock.10 mg/ml RNase A (Boil 10 mins, cool, filter and store at -20°C).4
西班牙酵母浸粉（Yeast－Extract－）與OXOID酵母浸粉LP0021技術..
西班牙酵母浸粉（Yeast Extract ）與OXOID酵母浸粉LP0021技術指標的對比西班牙pronadisa酵母浸粉（Yeast Extract ）是酵母提取物是酵母經過自溶、濃縮的水溶性部分。它當中含有豐富的B族維生素、各種氨基酸以及其它生長因子。它被用在許多微生物培養基制劑作為優良的
Layered－plates
General InformationThe cells being plated are sandwiched between layers of cell-free agar. The submerged cells grow into smaller colonies and many mor
Method:－Lymphoblastoid－Cell－Lines－from－Frozen－Whole－Blood
Method: Lymphoblastoid Cell Lines from Frozen Whole BloodMay 31, 1990Rosalie VeilePurpose:Blood Samples can be stored frozen as a backup in case an LC
Yeast－Nuclei－Isolation
This method gives yeast nuclei which look nearly purified microscopically. Nuclei isolated in this way do not give active transcription extracts when
Pectinase－assay
Pectinases are actually a mixture of enzymes, which, along with others such as cellulase, are widely used in the fruit juice industry where they are w
碳水化合物分析
Carbohydrate Assay?(Hancock Laboratory) (Accessible only by IE)This protocol is used to determine the relative amounts of LPS CHO present in a given s
酵母雙雜交系統
·?????????Yeast Two-Hybrid System?(Finley Lab)This is one of the most comprehensive and detailed guide to yeast two-hybrid system technique with intro
ChIP－using－plant－samples－–－Arabidopsis
實驗概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). T
DNA－Immunoprecipitation－for－the－Determination－of－DNABinding－Specificity
Andrea J. Gossett?and?Jason D. Lieb1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA1Corresponding autho
A－Method－for－MicroScale－Isolation－and－Purification－of－Gangliosides
A Method for Micro-Scale Isolation and Purification of GangliosidesStephan Ladisch~Director, Center for Cancer and Transplant Biology, Children's
Size－and－Shape－of－Protein－Molecules5
Hydrodynamic Analysis and EM Applied to Large Multisubunit ComplexesThe text box above showed the application of the Siegel–Monte analysis to SMC prot
Size－and－Shape－of－Protein－Molecules5
Hydrodynamic Analysis and EM Applied to Large Multisubunit ComplexesThe text box above showed the application of the Siegel–Monte analysis to SMC prot
CSF－Extract－Prep－for－Spindle－Assembly
This protocol is essentially as described by Murray (1991), Cell Cycle Extracts. In Methods in Cell Biology, B.K. Kay and B. Peng, eds. (San Diego: Ac
蛋白質相互作用
Interaction Trap/Trap Two-Hybrid System·?????????Yeast Two-Hybrid System?(Finley Lab)This is one of the most comprehensive and detailed guide to yeast
Coimmunoprecipitation－assays
co-IP assays can be performed between endogenous proteins or transiently or stably expressed exogenous - usually tagged - proteins. The advantage to u
Twohybrid－analysis－of－genetic－regulatory－networks
1. Introduction and BackgroundThere is a great need for general methods to characterize the proteins that contemporary biology makes available. The li
其它PCR方法
·?????????Standard PCR Protocol?(Molecular Biology Techniques Manual)The followings are described in detailRecommended Reagent ConcentrationsRecommend
Yeast－Media
YEPD (non-selection)-1% yeast extract-2% peptone-1.5% agar (if needed for plates)After autoclaving add glucose to 2% by adding 100 ml of 20% solution
酵母染色體沉淀分析方法
ABSTRACTThis protocol describes a method for the detection of proteins bound to specific regions of chromatin in yeast. There are many variations of t
Dynabeads?－CoImmunoprecipitation－Kit
實驗概要The ?Dynabeads? and buffers provided in this kit will enable you to a) ?covalently immobilize antibodies of your choice onto the surface of ?Dynab
Dynabeads?－CoImmunoprecipitation－Kit
實驗概要The ?Dynabeads? and buffers provided in this kit will enable you to a) ?covalently immobilize antibodies of your choice onto the surface of ?Dynab