ChIPusingplantsamples–Arabidopsis
實驗概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). The exact chromatin concentration should be determined before starting the X-ChIP assay. View our cross-linking chromatin immunoprecipitation (X-ChIP) protocol, which should be used after the chromatin preparation detailed below.實驗原理Eukaryotic chromatin is a co......閱讀全文
ChIP－using－plant－samples－–－Arabidopsis
實驗概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). T
ChIP－using－plant－samples
實驗概要?The ?immunoprecipitation (IP) of cross-linked chromatin with antibodies ?specific for certain histone modifications (chromatin ?immunoprecipitati
Studying－Arabidopsis－Envelope－Protein－Localization－and－Topology－Using－...
Chloroplasts are metabolically important organelles that perform many essential functions within plant cells. The chloroplasts can be subdivided i
GenomeWide－Identification－of－Transcription－FactorBinding－Sites－in...
Genome-Wide Identification of Transcription Factor-Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip)
Extraction－of－DNA－From－Plants－Using－Plant－DNAzol?－Reagent
實驗概要Plant DNAzol? is an extra-strength-DNAzol? reagent (patent pending) specifically formulated for the isolation of genomic DNA from plants. The Plan
Detection－of－Viruses－in－Infected－Plant－Extracts－using－ImmunocapturePCR
?1) Immunocapture stageCoating buffer: 15 mM Na2CO3; 35 mM NaHCO3, and 3 mM NaN3, per liter (pH 9.6).Extraction buffer: (20 mM Tris-HCL (pH 8.0), 138
Detection－of－Viruses－in－Infected－Plant－Extracts－using－ImmunocapturePCR
?1) Immunocapture stageCoating buffer: 15 mM Na2CO3; 35 mM NaHCO3, and 3 mM NaN3, per liter (pH 9.6).Extraction buffer: (20 mM Tris-HCL (pH 8.0), 138
Verification－of－In－...
實驗概要Environmental stresses often trigger rapid generation of reactive oxygen species (ROS) in plants. Excessive amount of ROS can cause damage to
DoseResponse－to－and－Systemic－Movement－of－Dexamethasone－in－the－GVGInducible
DoseResponse to and Systemic Movement of Dexamethasone in the GVG-Inducible Transgene System in ArabidopsisConstruction of transgenic plants is centra
Global－Expression－Profiling－of－RNA－from－Laser－Microdissected－Cells－at－...
Global expression profiling of RNA isolated from laser microdissected cells allows one to profile a specific set of cells allowing for enhanced se
包埋－Embedded－Samples
包埋 Embedded Samples可以將部分樣品包埋在1-4%濃度的低熔點瓊脂糖中，不同濃度的瓊脂糖其穩定性不同，對活體樣品的影響也不同。適合樣品：斑馬魚、果蠅、線蟲、擬南介等熒光標記的模式生物。包埋尺寸：平臺配有不同規格的毛細管和1ml注射器，樣品大小0.23mm-3.06mm，介于毛細管和注
懸掛－Hanging－Samples
懸掛 Hanging Samples大組織透明化樣品可以通過懸掛的方式放置在樣品載物臺上，樣品最大尺寸是1cm*1cm*1cm。適合樣品：水溶性試劑透明化的小鼠胚胎、腦、肺、肝臟、腎臟、胰腺、卵巢等。上樣方式：平臺配有不同形式的樣品粘托和樣品托盤，可以將樣品通過粘貼懸掛或者托掛的方式放置在載物臺上，
Tissue－preparation－protocol－for－ChIP
實驗概要This protocol ?describes how chromatin is prepared from tissue, which can subsequently ?be used for chromatin immunoprecipitation (ChIP). It is re
Transformation－Protocol－for－Arabidopsis
Transformation Protocol for Arabidopsis – AbbreviatedGerminate seed in pots↓ 4 weeksStreak bacteria onto YM/MinA↓ 2-3 days 28°CSpray/dip bacterial sus
Arabidopsis－gDNA－isolation
This is a simple and fast protocol for the extraction of genomic DNA from Arabidopsis thaliana that works fine in PCR for simple amplicons. We only us
In－Planta－Transformation－of－Arabidopsis
實驗概要? ? ? ? A breakthrough in Arabidopsis research was the invention ofthe vacuum-infiltration procedure, a simple and reliable methodof obtaining
Quantitating－RNA
RNA quantitation is an important and necessary step prior to most RNA analysis methods. Here we discuss three common methods used to quantitate RNA an
Use－of－Nonaqueous－Fractionation－and－Metabolomics－to－Study－Chloroplast－...
Use of Non-aqueous Fractionation and Metabolomics to Study Chloroplast Function in ArabidopsisChloroplasts are the chemical factories of plant cells b
Heterogeneity－of－SingleCell－Gene－Expression－Across－Phenotypically（二）
?Figure 2: Verification of C1 Single-Cell mRNA-Seq data quality. a)ERCC ?RNA Spike-In Control Mix 1 was applied to a C1 IFC at a total ?transcript in
圍封－Enclosed－Samples
?圍封 Enclosed Samples由于高濃度的瓊脂糖濃度會影響部分活體樣本的發育和狀態，瓊脂糖濃度太低又會影響樣品的穩定性，此時，我們可以選擇將樣品圍封在FEP管中。適合樣品：折射率和FEP、瓊脂糖相似的樣品，如斑馬魚、果蠅、線蟲、擬南介等。樣品大小：平臺配有不同規格的FEP管，具體圍封方式類
Estimation－of－Fat－in－Milk－Samples
實驗概要Milk fat measurement is a common task in the dairy industry, because milk fat content is one factor that determines milk price and is necessar
Estimation－of－Fat－in－Milk－Samples
IntroductionMilk fat measurement is a common task in the dairy industry, because milk fat content is one factor that determines milk price and is nece
Preparation－of－Stroma,－Thylakoid－Membrane,－and－Lumen－Fractions－from－...
Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic AnalysisFor many studies regarding
Total－Protein－Extraction－with－TCAAcetone
We describe a procedure allowing extraction of total proteins that performs efficiently with a large variety of plant tissues, based on simultaneo
Arabidopsis－RNA－extraction－protocol
1-2 g fresh material, freezer-dried, ground with 0.2g sand (if necessary), and then homogenized with 10ml RNA extraction buffer (see below).Spin at 8,
Simplified－Arabidopsis－Transformation－Protocol
實驗概要Our present protocol (Clough and Bent, 1998; modified from Bechtold et al. 1993) is extremely simple. We have found that the MS salts, hormone
Arabidopsis－RNA－extraction－protocol
1-2 g fresh material, freezer-dried, ground with 0.2g sand (if necessary), and then homogenized with 10ml RNA extraction buffer (see below). Spin
Cell－Suspension－Culture－of－Arabidopsis
實驗概要Cell Suspension Culture of Arabidopsis?主要試劑10% (v/v) Household BleachCallus Induction Medium?? ? ?Gamborg's B5 Basal Medium?? ? ?0.5 g/liter M
Simplified－Arabidopsis－Transformation－Protocol
(Brief version for those who are familiar with the method)Steve Clough and Andrew Bent, University of Illinois at Urbana-Champaign.Our present proto
ChIPChip－E.－coli
AbstractChIP-Chip stands for?Chromatin?Immunoprecipitation and chip in the sense of DNA microarray. It is a technique to determine the genome-wide bin