GenomeWideIdentificationofTranscriptionFactorBindingSitesin...
Genome-Wide Identification of Transcription Factor-Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip) or Sequencing (ChIP-seq)Nearly all signal transduction pathways lead to regulation of gene expression by controlling specific transcription factors (TFs). Chromatin immunoprecipitation (ChIP) is a powerful method for studying TF–DNA interactions in vivo. To identi......閱讀全文
GenomeWide－Identification－of－Transcription－FactorBinding－Sites－in...
Genome-Wide Identification of Transcription Factor-Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip)
軟件：Genomewide－Association－Study－Software
The software included are listed bellow?IMPUTEa program for genotype imputation in genome-wide association studies and fine-mapping?studies based on a
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement
IntroductionApoptosis is a normal physiological phenomenon put forward by Kerr [1]. It plays an important role in embryonic development, maintenance o
a－pipeline－for－the－identification－of－intact－Nglycopeptides（七）
Complementary ion information provided by HCD- and CID-MS/MS. Both HCD- and CID-MS/MScould be used to optimize the glycopeptide identification. Rece
Identification－and－expansion－of－the－tumorigenic－lung－cancer－stem－cell－...
Identification and expansion of the tumorigenic lung cancer stem cell populationLung cancer contains a rare population of CD133+ cancer stem-like ce
A－Yeast－Secretion－Trap－Assay－for－Identification－of－Secreted－Proteins－...
Secreted proteins from plants and phytopathogens play important roles in their interactions and contribute to elaborate mechanisms of attack, defe
a－pipeline－for－the－identification－of－intact－Nglycopeptides（五）
Alignment between MS/MS and MS3 identifications. Glycans were identified after analyzing the HCD/CID-MS/MS spectrum pairs, but the sequences of pe
a－pipeline－for－the－identification－of－intact－Nglycopeptides（二）
Methods?Materials. A uniform mixture of six standard glycoproteins was used as the starting material, including IgG (56834, catalog numeber), IgA
a－pipeline－for－the－identification－of－intact－Nglycopeptides（四）
Interpretation of MS3 data. MS3 data were converted to “.ms3” format by pXtract within pFind Studio (version 2.8)26,27, and then analyzed by pFind
a－pipeline－for－the－identification－of－intact－Nglycopeptides（一）
pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCDand CID-MS/MS and MS3?Wen-Feng Zeng1,2,*, Ming-Qi Liu3,*, Yang Zhang3,
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement2
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
a－pipeline－for－the－identification－of－intact－Nglycopeptides（三）
Figure 1. The overall workflow of pGlyco. First the sample is analyzed by HCD-MS/MS (NCE = 40%).Then the product-dependent CID-MS/MS and data-depend
a－pipeline－for－the－identification－of－intact－Nglycopeptides（六）
??Figure 4. Comparison of different decoy strategies. The estimated FDR of each decoy method is compared with the real FDR. “Decoy: + 11” means in
Identification－of－a－Mutant－Kinase/ATP－Analog－Pair1
Identification of a Mutant Kinase/ATP Analog PairScott T. Eblen, N. Vinay Kumar, and Michael J. WeberDepartment of Microbiology and Cancer Center, Uni
Identification－of－a－Mutant－Kinase/ATP－Analog－Pair2
Cellular Transfection and Immunoprecipitation??Before proceeding with the experiments outlined below, all kinase pocket mutants should be characterize
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites九
41. Schmidt O, Theopold U, Strand M: Innate immunity and its evasion and suppression by hymenopteran endoparasitoids. BioEssays 2001, 23(4):344–35
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites六
N-glycosylation modification of proteins has reported to improve the health of living organisms through antibacterial activity [68], antioxidant a
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites八
References1. Fujita T, Kozuka-Hata H, Ao-Kondo H, Kunieda T, Oyama M, Kubo T: Proteomic analysis of the royal jelly and characterization of the fu
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites二
ResultsIdentified novel royal jelly proteins ?To expand the number of known proteins in the RJ proteome, RJ proteins were extracted and digested wit
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites四
Figure 2 Distribution of N-glycopeptides analyzed by different enriched methods and instruments of royal jelly proteins. A is the distribution of N-
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites一
Comprehensive identification of novel proteins and N-glycosylation sites in royal jellyLan Zhang1,2?, Bin Han1?, Rongli Li1, Xiaoshan Lu1,3, Aiying Ni
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites三
?Note: All of the identified proteins are from Apis mellifera. Accession is the unique number given to mark the entry of a protein in the database
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites七
Data analysisTandem mass spectra were retrieved using Xcalibur (version 2.2, Thermo Fisher Scientific) and AnalystTF (version 1.6, AB SCIEX) softw
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites五
RJ provides efficient energetic fuels for the fast development of larvae and the egg-laying queen through the metabolism of sugars, lipids, and pr
二代測序鑒定鋁脅迫下野生大豆miRNA及其靶基因
野生大豆（Glycine soja）是世界上種植面積最廣的作物之一。相比于栽培大豆，野生大豆能夠更好地適應自然環境的脅迫如干旱，堿，鹽脅迫。對野生大豆的性狀進行系統研究，將有助于栽培大豆的遺傳改良。近日，華南農業大學亞熱帶農業生物資源保護與利用國家重點實驗室年海教授領銜的課題組聯合使用第二代高通量測
BMC－Plant－Biology測序鑒定鋁脅迫下野生大豆miRNA及其靶基因
野生大豆（Glycine soja）是世界上種植面積最廣的作物之一。相比于栽培大豆，野生大豆能夠更好地適應自然環境的脅迫如干旱，堿，鹽脅迫。對野生大豆的性狀進行系統研究，將有助于栽培大豆的遺傳改良。近日，華南農業大學亞熱帶農業生物資源保護與利用國家重點實驗室年海教授領銜的課題組聯合使用第二代高通
分子植物卓越中心等在甘薯多倍體遺傳定位研究中獲進展
近日，中國科學院分子植物科學卓越創新中心與上海師范大學、中國林業科學研究院等多家單位合作在Molecular Plant上發表題為Genome-wide identification of agronomically important genes in outcrossing crops us
水生所四膜蟲多基因家族分子進化研究取得新進展
BMC evolutionary biology雜志10月27日在線發表了中國科學院水生生物研究所原生動物功能基因組學學科組博士生熊杰完成的題為Genome-wide identification and evolution of ATP-binding cassette tran
有關梅花垂枝性狀遺傳的調控研究獲進展
梅花（Prunus mume ）是我國特有早春木本觀賞植物，素有“花魁”之美譽。垂枝梅因其枝條自然下垂、樹姿雋秀、花開如瀑，深受人們喜愛。然而，垂枝梅花品種資源稀缺，垂枝性狀遺傳機理仍不清晰，極大地限制了垂枝梅花新品種選育進程及其在園林景觀中的廣泛應用，垂枝性狀分子標記輔助選擇育種和遺傳機制的解
上海辰山植物園在分子進化研究方面取得新進展
3月3日，國際學術期刊BMC Genomics 在線發表了上海辰山植物園（中國科學院上海辰山植物科學研究中心）植物抗逆與分子進化研究組題為Genome-wide identification and evolutionary analyses of the PP2C gene family wi