Cultureofhumanvenoussmoothmusclecells
Culture of human venous smooth muscle cells 1. Explants of the 2 groups of skins (control subjects and patients with varicose veins) were prepared according to the method described for smooth muscle cells by carefully putting the epidermis at the top. 2. Cells were grown into collagen-precoated Petri dishes in Dulbecco’s modified Eagle medium supplemented with 10% fetal calf serum......閱讀全文
Culture－of－human－venous－smooth－muscle－cells
Culture of human venous smooth muscle cells???1.?Explants of the 2 groups of skins (control subjects and patients with varicose veins) were prepared
Isolation－and－Culture－of－human－vascular－smooth－muscle－cells
1.?Human vascular smooth muscle cells?(SMCs) were isolated from human saphenous vein.2.?Small moistened pieces of tissue (6 mm2) were placed intima
Isolation－and－culture－of－human－pulmonary－artery－smooth－muscle－cells－...
Isolation and culture of human pulmonary artery smooth muscle cells (PASMCs) 1.?The human pulmonary arteries were opened to expose the endothelia
Isolation－of－human－prostatic－smooth－muscle－cells
Human prostate tissues 1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of the prostate. 2.
Isolation－of－human－uterine－smooth－muscle－cells
1.?Approval to utilize human tissue was given by Hospital's Committee on Research Involving Human Subjects. 2.?Human myometrial and leiomyoma tissue
Primary－Culture－of－Pulmonary－Arterial－Smooth－Muscle－Cells
Primary Culture of Pulmonary Arterial Smooth Muscle Cells?1.Primary cultures of Pulmonary Arterial Smooth Muscle Cells (PASMCs) were isolated from hum
Isolation－and－culture－of－SpragueDawley－rat－aortic－smooth－muscle－cells
The intact mature arterial media is composed of at least four phenotypically unique cell subpopulations that reside in distinct medial layers.? T
Isolation－and－culture－of－smooth－muscle－cell
1.Male Wistar rats of about 250g body wt were anesthetized with ether and were decapitated. ?2.Kidneys and thoracic aorta were removed, rinsed with
Isolation－of－human－primary－airway－smooth－muscle
1.?Primary cultures of human primary airway smooth muscle (ASM) cells were prepared from explants of airway smooth muscle. 2.?The human trachea tiss
Mesenchymal－progenitor－cells－derived－from－human－muscle
Harvesting traumatized muscle derived mesenchymal progenitor cells (MPCs)1.?The muscle tissue was dissected without contaminating by granulation, adip
Culture－of－Human－Embryonic－Stem－Cells－(hESC)
All cell lines are initially grown according to the supplier's protocols but we are adapting them to one simple protocol outlined below: 6-well pl
Isolation－and－Culture－of－Human－Liver－Stem－Cells
Materials and Methods 1.?Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy liver tiss
Culture－of－human－renal－proximal－tubule－cells－and－renal－cortical－fibroblasts
1.?The method for primary culture of human renal proximal tubule cells (PTC) and renal cortical fibroblasts (CF) is described in detail.2.?Renal cor
Isolation－and－Culture－of－Multipotent－Stem－Cells－from－Human－Bone－Morrow
Bone marrow contains three types of stem cells:1.?Hematopoietic stem cells give rise to the three classes of blood cells that are found in the circu
Ex－Vivo－Human－Primary－Mesenchymal－Stem－Cells－(MSCs)－Culture
Human bone marrow contains mesenchymal progenitors (mesenchymal stem cells, MSCs).? MSCs produce adventitial cells in the human bone marrow microenvir
Different－types－of－human－cells
Human primary cells are cells taken from living human beings and cultured.? These cells retain the differentiation of the original cells taken in
Culture－of－BEND－Cells－(Bovine－Endometrial－Cells)
Culture of BEND Cells (Bovine Endometrial Cells) Charles E. Krininger, III and Peter J. Hansen? Dept. of Animal Sciences, University of Florida Thi
細胞技術專題：兔膀胱平滑肌細胞培養實驗（三）
?收起? 注意事項1. 應嚴格無菌操作。2. 仔細徹底剝除膀胱黏膜、黏膜下及漿膜層，是確保獲的大量高質單個膀胱平滑肌細胞的基礎。3. 膀胱平滑肌組織應盡量減碎以確保充分消化。4. 嚴格控制膠原酶和胰蛋白酶的濃度和消化時間，見消化液混濁、組織塊呈絮狀，或在倒置顯微鏡下見消化液中有較多單個細胞或細胞小
Human－B－cell－isolation－and－culture
B cell isolation1.?Donor blood was obtained with informed consent under guidelines issued by the Medical Ethics Committee. 2.?Mononuclear cell fractio
Human－B－cell－isolation－and－culture
實驗概要This protocol provides a general protocol for human B cell isolation and culture.實驗步驟B cell isolation1.?Donor blood was obtained with informed con
Barretts－esophageal－epithelial－and－fibroblast－primary－cultures
1.?Biopsy specimens for tissue culture were immediately placed on ice in primary cell culture system. 2.?Within 4 hours from the time of the biopsy,
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System Scott R. Hutton ?and? Larysa H. Pevny1 UNC Neurosc
Culture－of－retinal－endothelial－cells－and－pericytes
Isolation of retinal microvessels1.?Eyes were obtained and transported on ice.2.?Eyes were cut circumferentially 3 mm posterior to the limbus, the v
Isolation－and－culture－of－porcine－muller－cells
1.?Porcine eyes were harvested from pentobarbital-anesthetized animals, each weighing 30 to 40 pounds, and transported to the laboratory in ice-co
Immunofluorescence－Microscopy－of－tissue－culture－cells
Immunofluorescence Microscopy of tissue culture cellsThese methods are written for direct staining of filamentous actin with bodipy FL-phallicidin and
Isolation－and－culture－of－pancreatic－stellate－cells
Pancreatic stellate cells (PaSCs or PSCs) are myofibroblast-like cells found in the areas of the pancreas that have exocrine function.? PaSCs are
Culturing－Human－Neural－Stem－Cells
實驗概要 Neural ?stem cells (NSC) are valuable resources because of their ability to ?differentiate into neurons and glial cells with applications in ?n
Isolation－of－human－endometrial－epithelial－cells
Tissue collection1.?Endometrial biopsies were collected from women undergoing gynaecological procedures for benign conditions. 2.?All women reported
Isolation－of－Human－Pulmonary－Epithelial－Cells
?Isolation of Human Pulmonary Epithelial Cells????1.?Pulmonary epithelial cells from human lung tissue were isolated.2.?Lungs were perfused with 10
Isolation－of－human－atrial－or－ventricular－cells
1.?Human tissue was derived from atrial or ventricular biopsy specimens belonging to patients undergoing heart surgery.2.?Isolated myocardial tissue w