《干細胞》：人類骨髓干細胞新發現

來自杜蘭大學（Tulane University）健康科學中心，臺灣榮民總醫院（Veterans General Hospital-Taipei），陽明大學等處的研究人員利用缺氧的環境培養人類骨髓間葉性干細胞，取得了突破性的研究成果。這一研究成果公布在最新一期的國際干細胞權威期刊《干細胞》上。

原文檢索：
Stem Cells Vol. 25 No. 9 September 2007, pp. 2363 -2370
doi:10.1634/stemcells.2006-0686; www.StemCells.com
Angiogenic Effects of Human Multipotent Stromal Cell Conditioned Medium Activate the PI3K-Akt Pathway in Hypoxic Endothelial Cells to Inhibit Apoptosis, Increase Survival, and Stimulate Angiogenesis
[Abstract]

動物實驗雖證實移植骨髓間葉細胞可促進人體組織的修復與再生，但卻發現其在移植后著床于接受者的比率很低，因此使得這種干細胞移植的治療效果受到質疑。Shih-Chieh Hung等人發現，缺氧狀態下培養的間葉干細胞可增加移植后著床的成功率。在移植前將間葉干細胞放置于百分之一氧氣培養四十八小時，移植后著床的細胞數可增加三倍，大大提高了移植的成功率。

Shih-Chieh Hung還發現，間葉干細胞可存活于缺氧狀況并繼續增殖，但一般的已分化細胞卻會因缺氧凋亡。他舉血管內皮細胞為例說，間葉干細胞可拯救血管內皮細胞因缺氧所造成的死亡，同時可促進血管內皮細胞形成血管。干細胞會釋放生長因子及激素，包括血管內皮增生因子及第六型介白質，這些因子可以經由相關訊息傳遞路徑來抑制血管內皮細胞的凋亡及促進血管新生。

這一研究證實了人類骨髓間葉干細胞可分泌生長因子及激素來促進人體組織的修復與再生。使干細胞的應用將不受限于應用細胞治療造成著床及分化的機制來促進組織修復，同時也能應用其分泌因子來幫助組織再生。分離純化干細胞的分泌因子并加以應用，將有助于血管新生，并可用于治療因血流不足而造成的心肌梗塞、中風及缺血肢體等疾病。

附：
Shih-Chieh Hung, M.D.,Ph.D.

Birth place: Taipei, Taiwan

professor, Institute of Clinical Medicine, School of Medicine, National Yang-Ming University.

Investigator, Stem Cell Laboratory, Department of Medical Research and Education, Veterans General Hospital-Taipei

Visiting Staff, Orthopaedic surgeon, Veterans General Hospital-Taipei

Office Address: Stem Cell Laboratory. Department of Medical Research and Education, Veterans General Hospital-Taipei,

201, Sec. 2, Shih-Pai Road, Taipei, Taiwan

Office Telephone: +886-2-28757557 ext 118

FAX: +886-2-28265164

E-MAIL: hungsc@vghtpe.gov.tw

Education:

Medical School: School of Medicine, National Yang-Ming University, Taipei, Taiwan (1983/9~1990/6)

Internship and Residency: Veterans General Hospital-Taipei (1988/6~2000/6)

Graduate School (Ph.D. degree): Graduate School of Medicine, The University of Tokyo, Japan (National Scholarship) (1993/7~1997/6)

Linsensure:

Licensure: Medicine, Republic of China, 1990

Board Certification: Associate of Surgeon, Republic of China, 1998

Board Certification: Associate of Orthopaedic Surgeon, Republic of China, 1998

 

Post-Graduate Training:

Residency, Surgical Department, Fan-Lin Veterans Hospital, Hwa-Ling, Taiwan (1990/7~1992/7)

Residency Training: Department of Orthopaedics and Traumatology, Veterans General Hospital-Taipei (1992/7~2000/6)

Research Fellowship: Department of Orthopaedics, School of Medicine, The University of Tokyo (1993/7~1997/6)

Postdoctoral Fellowship: Department of Biochemistry, The University of Tokyo (1996/8~1997/6)

Visiting Investigator: Biomedical Institute, Sinica Academy (2003/1~2003/12)

Visiting Professor: Center for Gene Therapy, Tulane University Health Science Center: Prof. Prockop-DJ (2004/8-2005/7)

Reviewer for SCI papers:

Gene Therapy (2005)

Biochemical Journal (2005)

J Biomedical & Material Research (2006)

Publications

1. SC-Hung: Effects of unloading with motion on articular cartilage- an experimental study in adult rabbits. Degree of Ph.D., The University of Tokyo, 1997.

2. CL-Yu, KL-Chang, SC-Hung, BN-Chiang, SH-Han, SR-Wang: Defective helper T cell function in IgM rheumatoid factor synthesis in patients with ankylosing spondylitis. Scand J Rheumatology 18:43-49, 1989.

3. W-Ou, T-Kurokawa, K-Nakamura, T-Matsushida, R-Murashima, I-Kusaba, H-Okazaki, K-Mamada, R-Shiro, K-Tanaka, M-Takahashi, SC-Hung: Prevention of equinus deformity by brace and physiotherapy during leg lengthening. J Physical Medicine 6:164-167, 1995.

4. H-Okazaki, T-Kurokawa, K-Nakamura, K-Mamada, R-Shiro, K-Tanaka, W-Ou, SC-Hung, R-Murasima, I-Kusaba: Influence of length gain on axial deviation in tibial lengthening by callotasis method. 日本創外固定研究會誌 6:201-202, 1995. (Japanese Journal)

5. SC-Hung, T-kurokawa, K-Nakamura, T-Matsushita, R-Shiro, H-Okazaki: Narrowing of the hip joint space in patients with posttraumatic shortening of the femur. 東日本臨整會誌 7:47-47, 1995. (Japanese Journal)

6. K-Tanaka, T-Kurokawa, K-Nakamura, T-Matsushida, S-Horinaka, I-Kubasa, H-Okazaki, R-Shiro, W-Ou, SC-Hung: Callus formation in femur and tibia during leg lengthening. Acta Orthop Scand 67:158-160, 1996.

7. SC-Hung, T-kurokawa, K-Nakamura, T-Matsushita, R-Shiro, H-Okazaki: Narrowing of the joint space of the hip after traumatic shortening of the femur. J Bone Joint Surg 78B:718-721, 1996. (Corresponding author)

8. SC-Hung, K-Nakamura, T-Matsushita, H-Okazaki, R-Shiro, K-Mamada, K-Tanaka, W-Ou, T-kurokaw: Influence of femoral lengthening on hip joint space in post-traumatic femoral shortening. Acta Orthop Scand 68:541-544, 1997. (Corresponding author)

9. SC-Hung, K-Nakamura, R-Shiro, K-Tanaka, H-Kawahara, T-kurokawa: Effects of continuous distraction on cartilage in a moving joint: an investigation on adult rabbits. J Orthop Research 15:381-390, 1997. (Corresponding author)

10.S-Jinno, SC-Hung, H-Okayama: Cell cycle start from quiesence controlled by tyrosine phosphorylation of Cdk4. Oncogen 18:565-571, 1999.

11.S-Jinno, SC-Hung, H-Yamamoto, J-Lin, A-Nagata, H-Okayam: Oncogenic stumulation recruits cyclin-dependent kinase in the cell cycle start in rat fibroblast. Proc Natl Acad Sci USA 96:13197-13202, 1999.

12.HL-M, SC-Hung, ST-Wang, TH-Chen: The reoperation of failed rotator cuff repairs. J Chin Med Asso 66:96-102, 2003.