SingleCellPCR單細胞PCR實驗技術
Single Cell PCR(Protocol provided by Carolyn Troeger)Cell picking c Axiovert 100/Zeiss, extended glass capillary/Drummond, Broomall and a micromanipulator/ZeissTransfer cell into PCR tube containing 5 μl of 400 ng/μl Proteinase K/17 μM SDS (50 μl stock = 1 μl 20 mg/ml Prot K + 49 μl 0.005% SDS)Overlay c mineral oil if needed (depending on PCR machine) and incubate @ 50°C for 1 h, denaturate @ 99°C for 30 min.Keep tub......閱讀全文
Single－Cell－PCR－單細胞PCR實驗技術
Single Cell PCR(Protocol provided by Carolyn Troeger)Cell picking c Axiovert 100/Zeiss, extended glass capillary/Drummond, Broomall and a micromanipul
SingleCell－Electroporation－in－Xenopus
Single-Cell Electroporation in XenopusXue Feng Liu and Kurt HaasINTRODUCTIONSingle-cell electroporation (SCE) is a versatile technique for delivering
Single－tube－confirmation－PCR－protocol
The following colony PCR protocol has been designed to be performed in individual reaction tubes. We usually test three colonies from each transformat
Single－Primer－(SemiRandom)－PCR
DescriptionSingle primer PCR allows amplification from known to unknown regions in chromosomes, phage, plasmids, large PCR products and other sources
Preparation－of－cytospin－from－single－cell－suspension.
Cell number, speed and trivial details affect cytospin .Basic protocol:Prepare a cell suspension of not more than 0.5 x 106?cells / ml of protein-cont
Single－Cell－ICPMS－應用研究
癌癥研究 ? 環境毒理學 ? 生物技術和細胞科學 ? 藥物設計 ? 四組四極桿： · 第yi組 四極桿離子偏轉器（Q0，Quadrupole Ion Deflector）是一個基于離子能量的靜電質量分析器，對離子進行動態聚焦和質量篩選，同時把離子偏轉90度
Single－Cell－ICPMS－應用研究
? 癌癥研究? 環境毒理學? 生物技術和細胞科學? 藥物設計? 四組四極桿：·?第yi組四極桿離子偏轉器（Q0，Quadrupole Ion Deflector）是一個基于離子能量的靜電質量分析器，對離子進行動態聚焦和質量篩選，同時把離子偏轉90度以實現與中性成分和光子分離，導入下一級四極桿·?第二
Heterogeneity－of－SingleCell－Gene－Expression－Across－Phenotypically（一）
Introduction? Multi-cellular ?populations are fundamentally driven by the collective properties of ?individual cells. However, our understanding of ge
Heterogeneity－of－SingleCell－Gene－Expression－Across－Phenotypically（二）
?Figure 2: Verification of C1 Single-Cell mRNA-Seq data quality. a)ERCC ?RNA Spike-In Control Mix 1 was applied to a C1 IFC at a total ?transcript in
單細胞測序技術(single－cell－sequencing)－綜述（一）
單細胞生物學最近幾年是非常熱門的研究方向。在這一領域中，最前沿的則是單細胞測序技術。傳統測序方法一次處理成千上萬個細胞，得到的變異水平也是成千上萬個細胞的平均后水平。但是，就如同世界上沒有完全相同的兩片樹葉一樣，沒有兩個細胞是完全相同的。所以，單細胞測序對于研究單個細胞就顯得至關重要。單細胞測序可以
單細胞測序技術(single－cell－sequencing)－綜述（二）
DroNC-seq10月，Broad研究所張鋒教授團隊在Nature Methods上發表題為“Massively Parallel Single-Nucleus RNA-Seq with DroNc-Seq (doi: 10.1038/nmeth.4407)” 的文章，提出了一個新的測
單細胞凝膠電泳分析（single－cell－gel－eletrophoresis，SCGE）
單細胞凝膠電泳分析（single cell gel eletrophoresis，SCGE）是由Ostling等（1984）首創，后經Singh等（1988）進一步完善并逐漸發展起來的一種快速檢測單細胞 DNA損傷的實驗方法，適用于多種細胞，能夠靈敏地檢測DNA斷裂，在檢測誘變劑、射線等
Streaking－Bacteria－for－Single－Colonies
1. Initial inoculum:- From solid media, touch an isolated colonies with a sterile applicator or toothpick.- From liquid media, touch the culture with
single－atom－tips－(SATs)制作
目前普遍制作的SATs的方法主要有兩種：（1）構建法（2）選擇性氣蝕法。
Agarose－Gels－for－Single－Stranded－DNA
1. Prepare 50X TAE as:242 g Tris Base57.1 mL Glacial Acetic Acid100 mL 500 mM EDTA, pH 8.0600 mL ddH2OMix. Bring volume to 1 L. Autoclave.2. Mix the f
SingleVirus－Tracking－in－Live－Cells
Single-Virus Tracking in Live CellsMichael J. Rust, Melike Lakadamyali, Boerries Brandenburg and Xiaowei Zhuang?INTRODUCTIONReal-time, live-cell imagi
Multiple－studies－with－a－single－experiment:－The－Power－of－...（四）
Better Ion Transmission With Segmented Quadrupole?Segmented Quadrupole ?Improved transmission across m/z range and for narrow windows?Brighter Source
Multiple－studies－with－a－single－experiment:－The－Power－of－－...（一）
Multiple studies with a single experiment: The Power of Quantitative MultiplexingMultiple studies with a single experiment: The Power of Quantitative
Multiple－studies－with－a－single－experiment:－The－Power－of－...（六）
??SCX and high pH reversed phase fractionation are both orthogonal to low pH C18 LC separation?Strong cation exchange (SCX) requires sample desalting
Multiple－studies－with－a－single－experiment:－The－Power－of－...（七）
SPS TMT Method Development- What is New on Lumos?Multiplexing On the Benchtop Orbitrap System (Q Exactive Series)??Take advantage of segmented quadrup
SNPs(single－nucleotide－polymorphism)的概念
SNPs(single nucleotide polymorphism ， SNP ，發音為 “snips”), 主要是指在基因組水平上由單個核苷酸的變異所引起的 DNA 序列多態性。它是人類可遺傳的變異中最常見的一種。占所有已知多態性的 90% 以上。 SNP 在人類基因組中廣泛存在，平均
Multiple－studies－with－a－single－experiment:－The－Power－of－...（二）
A Better Multiplexing Method– Isobaric Mass Tagging??Less MS1 Complexity?????Increased Throughput Concurrent MS analysis of multiple samples?????Less
Multiple－studies－with－a－single－experiment:－The－Power－of－...（五）
Competitive Advantages?Trust your quantitation!?Multinotch MS3 quantitation is more accurate than other MS2 Methods?The accuracy of Multinotch MS3 qua
Multiple－studies－with－a－single－experiment:－The－Power－of－...（三）
Ratio Distortion with Isobaric MultiplexingProblem: Quantitation of low-abundance proteins in a complex background is distorted by co-isolated interfe
Fluidigm公司微液流芯片在單細胞研究中的應用（二）
It was also worth it for Tannishtha Reya, who studies stem cell fate and cancer at Duke University Medical Center in Durham, North Carolina. "Im
SingleChain－Fv－Antibodies－Expressed－in－Plants
Methods of gene cloning and genetic engineering of immunoglobulins and transgenic plant techniques have given rise to new approaches in plant biot
Detection－of－MicroRNA－Heterogeneity－in－Single－Cells－Using－an－Automated
Introduction ?MicroRNA ?(miRNAs) are short (18–24 nucleotides), non-coding RNAs that regulate ?gene expression by both disrupting messenger RNA (mRNA
單向瓊脂擴散試驗（Single－immunodiffusion－test）
可溶性抗原與相應抗體在半固體瓊脂凝膠內擴散，二者相遇，在比例合適處形成白色沉淀。單向瓊脂擴散試驗（Single immunodiffusion test）—人血清中IgG、IgA、IgM的測定。??? 單向瓊脂擴散試驗是一種定量試驗，將抗體混合于瓊脂內，傾注于玻片或平皿上，凝固后在瓊脂上打孔，再將抗
單細胞的可靠全基因組擴增
目前多種新型分析技術，如新一代測序和芯片，都是為細胞群體而設計的，需要一定的樣本量。對于一些臨床或法醫樣本，如腫瘤細胞或循環胎兒細胞等，它們的細胞數量有限，直接限制了其下游應用。例如，在分析腫瘤細胞中的體細胞DNA突變或單個細菌基因組時，單細胞中的DNA無法滿足測序的mg級樣品量需求。為了從少量樣品
ASENSITIVE－METHOD－FOR－DETECTION－OF－APOPTOSIS－BY－SINGLE－LASER－FLOW－CYTOMETRY
MATERIALS:1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide)2. 7-Amino-actinomycin D (7-